LOCUS       BBa_J10059               912 bp    DNA     linear       26-Nov-2024
DEFINITION  pBad+BD18+RFP_withoutATG
ACCESSION   BBa_J10059
VERSION     BBa_J10059.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     RBS             157..165
                     /label=leader RBS
     misc_feature    171..173
                     /label=Start
     misc_feature    222..224
                     /label=Stop
     misc_feature    224..226
                     /label=Start
     RBS             209..217
                     /label=GOI RBS
     misc_feature    139..226
                     /label=BBa_J119024
     misc_feature    235..237
                     /label=second codon
     CDS             235..906
                     /label=ORF no ATG
     misc_feature    907..912
                     /label=double stop
     misc_feature    235..912
                     /label=BBa_J100051
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagaggg gcccaagttc acttaaaaag gagatcaaca atgaaagcaa
      181 ttttcgtact gaaacatctt aatcatgcga cggagcgttt ctaatgtact agaggcttcc
      241 tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac
      301 ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc
      361 gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg
      421 cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg
      481 aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt
      541 gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa
      601 ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg
      661 gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg
      721 cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct
      781 aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc
      841 cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc
      901 ggtgcttaat aa
//