##gff-version 3
##sequence-region BBa_J10059 1 912
BBa_J10059	.	engineered_region	139	226	.	+	0	ID=BBa_J119024;Name=BBa_J119024
BBa_J10059	.	start_codon	171	173	.	+	0	ID=annotation2167441;Name=Start;Parent=BBa_J119024
BBa_J10059	.	stop_codon	222	224	.	+	0	ID=annotation2167442;Name=Stop;Parent=BBa_J119024
BBa_J10059	.	ribosome_entry_site	157	165	.	+	0	ID=annotation2167440;Name=leader RBS;Parent=BBa_J119024
BBa_J10059	.	ribosome_entry_site	209	217	.	+	0	ID=annotation2167444;Name=GOI RBS;Parent=BBa_J119024
BBa_J10059	.	start_codon	224	226	.	+	0	ID=annotation2167443;Name=Start;Parent=BBa_J119024
BBa_J10059	.	engineered_region	235	912	.	+	0	ID=BBa_J100051;Name=BBa_J100051
BBa_J10059	.	stop_codon	907	912	.	+	0	ID=annotation2169535;Name=double stop;Parent=BBa_J100051
BBa_J10059	.	point_mutation	235	237	.	+	0	ID=annotation2169533;Name=second codon;Parent=BBa_J100051
BBa_J10059	.	CDS	235	906	.	+	0	ID=annotation2169534;Name=ORF no ATG;Parent=BBa_J100051
BBa_J10059	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453
>BBa_J10059
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagaggggcccaagttcacttaaaa
aggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatgtactagaggct
tcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaa
tcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgcc
gttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggac
tacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgtta
cccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtcc
ggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaa
atcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccgg
ttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaaca
gtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa