BBa_J119024 1 BBa_J119024 BD18 bicistronic translational junction - Medium effiency 2011-12-21T12:00:00Z 2015-10-01T11:42:24Z Synthetic DNA. The BD18 bicistronic translational junction was engineered by Vivek Mutalik and The BIOFAB Team at biofab.org. It includes an RBS that allows translation of a leader polypeptide. The leader has a second RBS embedded in it. The stop codon for the leader polypeptide overlaps with the start codon for the gene of interest. false false _613_ 606 606 61 Not in stock false The BD18 bicistronic translational junction was designed to give reproducible and consistent expression for various genes of interest. false Vivek Mutalik annotation2167440 1 leader RBS range2167440 1 19 27 annotation2167441 1 Start range2167441 1 33 35 annotation2167442 1 Stop range2167442 1 84 86 annotation2167444 1 GOI RBS range2167444 1 71 79 annotation2167443 1 Start range2167443 1 86 88 BBa_I13453 1 BBa_I13453 Pbad promoter 2005-05-24T11:00:00Z 2015-08-31T04:07:34Z Released HQ 2013 PBad promoter from I0500 without AraC. false false _11_ 0 253 6 In stock false true jkm BBa_J10059 1 BBa_J10059 pBad+BD18+RFP_withoutATG 2012-01-10T12:00:00Z 2015-08-31T04:08:24Z from registry parts, to be synthesized de novo. This device is used to compare the output from different promoters when using BD18 and E1010. false false _578_120_ 0 201 61 Not in stock false Had to delete ATG from E1010. false Malcolm Campbell component2169546 1 BBa_J100051 component2169542 1 BBa_J119024 component2169536 1 BBa_I13453 annotation2169542 1 BBa_J119024 range2169542 1 139 226 annotation2169546 1 BBa_J100051 range2169546 1 235 912 annotation2169536 1 BBa_I13453 range2169536 1 1 130 BBa_J100051 1 BBa_J100051 RFP (E1010) with start codon deleted 2012-01-10T12:00:00Z 2015-08-31T04:08:22Z Synthesized from E1010 template. Had to create a new part in order to make the final construct that will be synthesized. Could not edit the device sequence manually. false false _578_120_ 0 201 61 It's complicated false Needed to remove ATG for C-dog expression system. false Malcolm Campbell annotation2169534 1 ORF no ATG range2169534 1 1 672 annotation2169535 1 double stop range2169535 1 673 678 annotation2169533 1 second codon range2169533 1 1 3 BBa_I13453_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc BBa_J10059_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatgtactagaggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa BBa_J100051_sequence 1 gcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa BBa_J119024_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z