LOCUS       BBa_J10061               820 bp    DNA     linear       25-Jan-2026
DEFINITION  pTet+BD18+RFP_withoutATG no scars
ACCESSION   BBa_J10061
VERSION     BBa_J10061.1  
FEATURES             Location/Qualifiers
     promoter        1..54
                     /label=pTet R0040
     misc_feature    55..142
                     /label=BD18 J119024
     CDS             143..820
                     /label=RFP J100051
     misc_feature    815..820
                     /label=double stop
     misc_feature    140..142
                     /label=BD18 start
     misc_feature    138..140
                     /label=BD18 stop
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacgggccc
       61 aagttcactt aaaaaggaga tcaacaatga aagcaatttt cgtactgaaa catcttaatc
      121 atgcgacgga gcgtttctaa tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt
      181 caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga
      241 aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct
      301 gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa
      361 acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga
      421 acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca
      481 agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc
      541 ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga
      601 cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga
      661 cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa
      721 aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta
      781 cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa
//