LOCUS       BBa_J10063              2623 bp    DNA     linear       22-Dec-2024
DEFINITION  Green Firefly Luciferase and LRE L. Cruciata (E. coli optimised)
ACCESSION   BBa_J10063
VERSION     BBa_J10063.1  
FEATURES             Location/Qualifiers
     RBS             1..17
                     /label=endogenous RBS
     misc_feature    945..950
                     /label=double stop
     misc_feature    18..20
                     /label=LRE ORF
     CDS             18..950
                     /label=Luciferin Regenerating Enzyme
     misc_feature    951..956
                     /label=BamH 1 site
     RBS             957..973
                     /label=endogenous RBS
     misc_feature    974..976
                     /label=green luciferase ATG
     CDS             974..2623
                     /label=green luciferase
     misc_feature    2618..2623
                     /label=double stop
     misc_feature    1828..1828
                     /label=T --> C kill Spe I site
ORIGIN
        1 ttaaggaggt aaaaaaaatg gccccgaccg tggaacaaat tgtggagctg ggtacgtatc
       61 tgctggccga aagcccgcac tgggacgatg aaacgcaaag cctgtatttc gtcgatatcg
      121 ttggtcgcag cgtgaacaaa tacgttccga cgaccaagac gcatacccaa ctgaaattcg
      181 acaagaatcc gagcttcatc atcccggtga agggttgttc cgaccgtttt atcgtcagcc
      241 tggaacgcga gatcaacctg ctgacctggg acggcgcatc ctctgcacca tccaagattg
      301 agaagatcgc ggttttcgat aatacgccag agaagagcga aaaccgtctg aacgatggta
      361 aggcagatcc gctgggcaac ctgtgggcgg gtaccatgaa tatgggttct gaccatacca
      421 ccggcacgcc ggtgcgcggt accctgagca gcctgagcaa caagcaagtc aaagagcacg
      481 tcagcgaggt ctgcatcagc aatggcctgg catggagcaa ggacctgaag aaattctatt
      541 acattgatag cgctgttcgt caagttgacc agttcgattt cgatgcgaaa aacctgagct
      601 tgagcaatcg tcaaccgctg ttcacctttg acaaacacgg catcatgggt agcccggatg
      661 gtcagaccat cgatgcggag ggtaacctgt gggttgccac ctgtcagggc gataaagtcc
      721 tgaagattga tacttctacc ccggaaaccc tgctgggtat tgtggagatt ccggaacatc
      781 aggtgacgag cgtttgcatc ggcggtgcag aactgaacgt tttgtatgtg acgaccgcgt
      841 ccattaaact gcctggtgcg gatgaaacca agccgatgaa aggcgccatc tataaagtca
      901 ccggtctggg tgtcaaaggt ctgccgggtg accgtgttaa gctgtaataa ggatccttaa
      961 ggaggtaaaa aaaatggaga acatggagaa cgacgagaat attgtggttg gtccgaaacc
     1021 gttctacccg atcgaagagg gcagcgcggg tacccagctg cgtaaataca tggaacgcta
     1081 cgcgaaactg ggtgcgatcg cgttcacgaa tgcggttacc ggcgtggact attcctatgc
     1141 ggagtatttg gaaaagtcgt gttgtctggg caaggcgtta cagaattatg gcctggtggt
     1201 cgatggccgt atcgcgctgt gcagcgagaa ctgtgaagag tttttcattc cggtgatcgc
     1261 gggtttgttt attggcgtgg gcgttgcacc gaccaatgaa atctacacgc tgcgcgaact
     1321 ggtgcactcc ctgggtatct ctaagccgac gatcgttttc agctccaaga aaggtttgga
     1381 taaagttatt acggtgcaaa agacggtcac cacgatcaaa acgattgtga tcctggacag
     1441 caaagttgat taccgcggtt atcaatgcct ggatacgttc atcaagcgta acaccccgcc
     1501 aggttttcaa gcatctagct tcaaaacggt ggaggttgac cgtaaagaac aagtcgcact
     1561 gattatgaat agctcgggta gcacgggcct gccaaagggt gtgcagctga cccatgaaaa
     1621 caccgttacc cgttttagcc acgcgcgtga tccgatttac ggcaatcaag tgtcgccggg
     1681 tacggctatt ctgactgttg tcccttttca ccatggtttt ggcatgttta ccactctggg
     1741 ttacctgatt tgcggttttc gcgtcgttat gctgaccaag tttgatgaag aaaccttcct
     1801 gaaaaccctg caagactaca agtgcaccag tgtgattctg gtcccgacgc tgttcgcgat
     1861 cctgaacaaa agcgagctgc tgaataagta cgacctgagc aatttggtgg agattgcgag
     1921 cggcggtgct ccgttgagca aagaagtagg cgaggcagtg gcacgtcgct ttaacctgcc
     1981 tggcgtccgt caaggttacg gtttgactga aaccacgagc gcgattatca tcaccccgga
     2041 aggtgacgat aaacctggcg ctagcggtaa ggtcgtgccg ttgttcaagg ctaaagttat
     2101 tgatctggac accaagaaga gcctgggccc gaatcgccgt ggtgaagttt gcgttaaggg
     2161 cccgatgctg atgaaaggtt atgtaaacaa tccggaagca acgaaagagc tgatcgacga
     2221 agagggctgg ctgcacactg gcgatatcgg ctactacgat gaagagaaac actttttcat
     2281 tgttgatcgc ctgaaaagcc tgatcaagta caagggttac caagtcccgc cagcggagct
     2341 ggaaagcgta ctgttgcagc acccgtcgat tttcgacgcc ggtgtggctg gtgtgcctga
     2401 tccggtggct ggcgaactgc cgggtgctgt cgtggtgctg gagagcggta agaatatgac
     2461 cgaaaaagaa gtcatggact atgtggcgag ccaagtgtcc aatgcaaaac gtctgcgtgg
     2521 cggtgttcgc tttgtggacg aagtgccgaa aggtctgacc ggcaaaattg acggtcgtgc
     2581 aattcgtgag attctgaaga aaccggtcgc gaaaatgtaa taa
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