BBa_J10063 1 BBa_J10063 Green Firefly Luciferase and LRE L. Cruciata (E. coli optimised) 2012-01-10T12:00:00Z 2015-08-31T04:08:24Z from registry, synthesized de novo. The original part (K325229) was used as the template. We have removed all aspects of the pBad promoter and AraC regulatory DNA. This part now starts with the RBS in part K325229. K325229 is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase. Then a Val239Ile mutation was used to create a green bioluminescence. false false _578_120_ 0 201 61 Not in stock false Wanted to allow users to use their favorite promoter. false Malcolm Campbell annotation2169567 1 LRE ORF range2169567 1 18 20 annotation2169571 1 green luciferase ATG range2169571 1 974 976 annotation2169570 1 endogenous RBS range2169570 1 957 973 annotation2169574 1 T --> C kill Spe I site range2169574 1 1828 1828 annotation2169568 1 Luciferin Regenerating Enzyme range2169568 1 18 950 annotation2169569 1 BamH 1 site range2169569 1 951 956 annotation2169565 1 endogenous RBS range2169565 1 1 17 annotation2169573 1 double stop range2169573 1 2618 2623 annotation2169572 1 green luciferase range2169572 1 974 2623 annotation2169566 1 double stop range2169566 1 945 950 BBa_J10063_sequence 1 ttaaggaggtaaaaaaaatggccccgaccgtggaacaaattgtggagctgggtacgtatctgctggccgaaagcccgcactgggacgatgaaacgcaaagcctgtatttcgtcgatatcgttggtcgcagcgtgaacaaatacgttccgacgaccaagacgcatacccaactgaaattcgacaagaatccgagcttcatcatcccggtgaagggttgttccgaccgttttatcgtcagcctggaacgcgagatcaacctgctgacctgggacggcgcatcctctgcaccatccaagattgagaagatcgcggttttcgataatacgccagagaagagcgaaaaccgtctgaacgatggtaaggcagatccgctgggcaacctgtgggcgggtaccatgaatatgggttctgaccataccaccggcacgccggtgcgcggtaccctgagcagcctgagcaacaagcaagtcaaagagcacgtcagcgaggtctgcatcagcaatggcctggcatggagcaaggacctgaagaaattctattacattgatagcgctgttcgtcaagttgaccagttcgatttcgatgcgaaaaacctgagcttgagcaatcgtcaaccgctgttcacctttgacaaacacggcatcatgggtagcccggatggtcagaccatcgatgcggagggtaacctgtgggttgccacctgtcagggcgataaagtcctgaagattgatacttctaccccggaaaccctgctgggtattgtggagattccggaacatcaggtgacgagcgtttgcatcggcggtgcagaactgaacgttttgtatgtgacgaccgcgtccattaaactgcctggtgcggatgaaaccaagccgatgaaaggcgccatctataaagtcaccggtctgggtgtcaaaggtctgccgggtgaccgtgttaagctgtaataaggatccttaaggaggtaaaaaaaatggagaacatggagaacgacgagaatattgtggttggtccgaaaccgttctacccgatcgaagagggcagcgcgggtacccagctgcgtaaatacatggaacgctacgcgaaactgggtgcgatcgcgttcacgaatgcggttaccggcgtggactattcctatgcggagtatttggaaaagtcgtgttgtctgggcaaggcgttacagaattatggcctggtggtcgatggccgtatcgcgctgtgcagcgagaactgtgaagagtttttcattccggtgatcgcgggtttgtttattggcgtgggcgttgcaccgaccaatgaaatctacacgctgcgcgaactggtgcactccctgggtatctctaagccgacgatcgttttcagctccaagaaaggtttggataaagttattacggtgcaaaagacggtcaccacgatcaaaacgattgtgatcctggacagcaaagttgattaccgcggttatcaatgcctggatacgttcatcaagcgtaacaccccgccaggttttcaagcatctagcttcaaaacggtggaggttgaccgtaaagaacaagtcgcactgattatgaatagctcgggtagcacgggcctgccaaagggtgtgcagctgacccatgaaaacaccgttacccgttttagccacgcgcgtgatccgatttacggcaatcaagtgtcgccgggtacggctattctgactgttgtcccttttcaccatggttttggcatgtttaccactctgggttacctgatttgcggttttcgcgtcgttatgctgaccaagtttgatgaagaaaccttcctgaaaaccctgcaagactacaagtgcaccagtgtgattctggtcccgacgctgttcgcgatcctgaacaaaagcgagctgctgaataagtacgacctgagcaatttggtggagattgcgagcggcggtgctccgttgagcaaagaagtaggcgaggcagtggcacgtcgctttaacctgcctggcgtccgtcaaggttacggtttgactgaaaccacgagcgcgattatcatcaccccggaaggtgacgataaacctggcgctagcggtaaggtcgtgccgttgttcaaggctaaagttattgatctggacaccaagaagagcctgggcccgaatcgccgtggtgaagtttgcgttaagggcccgatgctgatgaaaggttatgtaaacaatccggaagcaacgaaagagctgatcgacgaagagggctggctgcacactggcgatatcggctactacgatgaagagaaacactttttcattgttgatcgcctgaaaagcctgatcaagtacaagggttaccaagtcccgccagcggagctggaaagcgtactgttgcagcacccgtcgattttcgacgccggtgtggctggtgtgcctgatccggtggctggcgaactgccgggtgctgtcgtggtgctggagagcggtaagaatatgaccgaaaaagaagtcatggactatgtggcgagccaagtgtccaatgcaaaacgtctgcgtggcggtgttcgctttgtggacgaagtgccgaaaggtctgaccggcaaaattgacggtcgtgcaattcgtgagattctgaagaaaccggtcgcgaaaatgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z