BBa_J10064 1 BBa_J10064 Lux Operon Vibrio fischeri from part K325909 2012-01-10T12:00:00Z 2015-08-31T04:08:24Z from K325909 with pBad removed, synthesized de novo This page described the lux operon from Vibrio fischeri. To relieve LuxR control we placed Lux C, D, A, B, E under a promoter of your choice. A more complete description can be found on the [[http://2010.igem.org/Team:Cambridge Cambridge iGEM 2010] website. This Bacterial lux operons encodes five enzymes involved in the light-generating pathway. LuxA and LuxB encode the two subunits of the bacterial luciferase, while the products of LuxC, LuxD and LuxE synthesise the substrate for the light emitting reaction, tetradecanal. The exact function of LuxG is unknown, and it appears to be non-essential for light emission, but its presence increases light output. In nature, the lux genes appear to be repressed by the nucleoid protein, H-NS, and occur under quorum sensing control. We removed the natural quorum sensing control to facilitate use of the part in biosensors under different regulatory inputs. As of October 2010 [http://2010.igem.org/Team:Cambridge Cambridge] believe this is the first and only BioBrick to emit light in normal E. coli strains without the addition of any external substrate. false true _578_120_ 0 201 61 It's complicated false removed pBad to make it more usable for different purposes false Malcolm Campbell annotation2169585 1 endogenous #6? range2169585 1 5705 5709 annotation2169578 1 luxD range2169578 1 1465 2369 annotation2169576 1 luxC range2169576 1 11 1450 annotation2169583 1 endogenous #5 range2169583 1 4565 4573 annotation2169575 1 endogenous #1 range2169575 1 1 10 annotation2169582 1 luxB range2169582 1 3522 4502 annotation2169580 1 luxA range2169580 1 2419 3483 annotation2169584 1 luxE range2169584 1 4577 5713 annotation2169579 1 endogenous #3 range2169579 1 2404 2413 annotation2169586 1 uxG range2169586 1 5717 6427 annotation2169577 1 endogenous #2 range2169577 1 1451 1462 annotation2169581 1 endogenous #4 range2169581 1 3507 3518 BBa_J10064_sequence 1 agggaaaataatgaataaatgtattccaatgataattaatggaatgattcaagattttgataattatgcatataaagaagttaaactaaataatgataatagagtaaaattatctgtcattactgaaagttcagtttcaaaaacattaaatatcaaagatagaattaatctaaatttaaatcagattgtgaattttttatataccgttggtcaacgatggaaaagtgaagaatataatcggcgacgaacctatatccgtgagttaaaaacatatcttggttattctgatgaaatggcaagattagaagcgaattggattgcaatgttattgtgctctaaaagtgctttgtatgacattgttaattatgatttgggctctatacacgtattagatgaatggcttccacgtggtgattgctatgttaaagcacaaccgaaaggtgtttctgttcacttgttagctggtaatgttccattatcaggagtgacatctattttgcgtgctattttaacaaaaaatgagtgcattattaaaacttcgtcttcagatccttttactgcaaacgctttagtttccagttttattgatgttaatgcagaccatccaataaccaaatcaatgtctgttatgtattggccgcatgatgaagatatgactctatctcaaagaataatgaatcatgccgacgtggttattgcttggggtggagacgaggcgattaaatgggcggtaaaatattcaccaccgcatgtcgatattctgaaatttggaccaaagaaaagcttaagtattattgaagctcctaaagatatagaagccgcagcaatgggggttgctcatgatatttgtttctatgaccagcaagcctgcttctctactcaagacgtttattatataggagataatttacctttatttttaaatgaacttgaaaaacagctagatcgatacgcgaaaattttaccaaaaggttcaaatagttttgatgaaaaagcggcgtttactcttactgaaaaagaaagtctatttgctggatatgaagtgagaaagggagataagcaagcttggttaatagtcgtatcacctacaaatagctttggaaatcaaccgctatcacgaagtgtgtatgttcatcaagtatctgatattaaagagataattccttttgttaataaaaatagaacacaaactgtttctatttatccttgggaagcgtcattaaaatatcgagataaattagcaagaagtggagttgaaagaattgttgaatcaggcatgaataatattttcagagttggaggggctcatgattcattatctcctctccagtacctagttaggtttgtatcgcatgagagaccatttaattatacgacaaaagatgttgcggttgaaatcgaacaaacacgttacttagaggaagataagtttttagtttttgtcccatagttaaaggaaattatatgaaagatgaaagtgctttttttacgattgatcacatcatcaagcttgataatggtcagtctatccgagtttgggaaacactccctaaaaagaacgtaccagagaaaaaacatacaatacttattgcttcgggttttgctagaagaatggatcattttgcaggtcttgctgagtatttatctactaacggttttcatgtcattcgctacgattctttgcatcatgttggattaagcagtggatgtataaatgaatttacgatgtcgattggaaaaaatagcctgcttacagtcgtagattggcttacagatcatggtgtcgaacgaatagggctgattgctgctagtttgtcagcgagaatcgcctatgaggtagtaaataaaattaaattatcatttttaattacggccgtaggtgtcgttaatcttagagatacattagaaaaagcattggagtatgactatttgcaattacctatttcagagttaccagaagatcttgactttgaaggtcataatttaggatcggaggtctttgttacagattgctttaaacataattgggacacgttagactcgacacttaatagtgttaaaggattagcgattccatttattgcttttactgcaaacgatgatagttgggtaaagcaaagtgaagttatagagctcattgatagcattgaatctagtaattgtaagctctattcgctaattggaagttcacatgatcttggggaaaatttggttgtattaagaaatttttatcaatcagtgacgaaggcagccttagcattagatgatggtttattggatttagagatagacattattgaacctcgatttgaggacgttacaagtattactgttaaggagcgtagattaaaaaatgaaattgaaaatgaattattagaattggcttaaataaacagaatcaccaaaaaggaatagagtatgaagtttggaaatatttgtttttcgtatcaaccaccaggtgaaactcataagcaagtaatggatcgctttgttcggcttggtatcgcctcagaagaggtagggtttgatacatattggaccttagaacatcattttacagagtttggtcttacgggaaatttatttgttgctgcggctaacctgttaggaagaactaaaacattaaatgttggcactatgggggttgttattccgacagcacacccagttcgacagttagaagacgttttattattagatcaaatgtcgaaaggtcgttttaattttggaaccgttcgagggctataccataaagattttcgagtatttggtgttgatatggaagagtctcgagcaattactcaaaatttctaccagatgataatggaaagcttacagacaggaaccattagctctgatagtgattacattcaatttcctaaggttgatgtatatcccaaagtgtactcaaaaaatgtaccaacctgtatgactgctgagtccgcaagtacgacagaatggctagcaatacaagggctaccaatggttcttagttggattattggtactaatgaaaaaaaagcacagatggaactctataatgaaattgcgacagaatatggtcatgatatatctaaaatagatcattgtatgacttatatttgttctgttgatgatgatgcacaaaaggcgcaagatgtttgtcgggagtttctgaaaaattggtatgactcatatgtaaatgcgaccaatatctttaatgatagcaatcaaactcgtggttatgattatcataaaggtcaatggcgtgattttgttttacaaggacatacaaacaccaatcgacgtgttgattatagcaatggtattaaccctgtaggcactcctgagcagtgtattgaaatcattcaacgtgatattgatgcaacgggtattacaaacattacatgcggatttgaagctaatggaactgaagatgaaataattgcttccatgcgacgctttatgacacaagtcgctcctttcttaaaagaacctaaataaattacttatttgatactagagataataaggaacaagttatgaaatttggattattttttctaaactttcagaaagatggaataacatctgaagaaacgttggataatatggtaaagactgtcacgttaattgattcaactaaatatcattttaatactgcctttgttaatgaacatcacttttcaaaaaatggtattgttggagcacctattaccgcagctggttttttattagggttaacaaataaattacatattggttcattaaatcaagtaattaccacccatcaccctgtacgtgtagcagaagaagccagtttattagatcaaatgtcagagggacgcttcattcttggttttagtgactgcgaaagtgatttcgaaatggaattttttagacgtcatatctcatcaaggcaacaacaatttgaagcatgctatgaaataattaatgacgcattaactacaggttattgtcatccccaaaacgacttttatgattttccaaaggtttcaattaatccacactgttacagtgagaatggacctaagcaatatgtatccgctacatcaaaagaagtcgtcatgtgggcagcgaaaaaggcactgcctttaacatttaagtgggaggataatttagaaaccaaagaacgctatgcaattctatataataaaacagcacaacaatatggtattgatatttcggatgttgatcatcaattaactgtaattgcgaacttaaatgctgatagaagtacggctcaagaagaagtgagagaatacttaaaagactatatcactgaaacttaccctcaaatggacagagatgaaaaaattaactgcattattgaagagaatgcagttgggtctcatgatgactattatgaatcgacaaaattagcagtggaaaaaacagggtctaaaaatattttattatcctttgaatcaatgtccgatattaaagatgtaaaagatattattgatatgttgaaccaaaaaatcgaaatgaatttaccataataaaattaaaggcaatttctatattagattgcctttttaaatttctgttgatattaggtattactggagagggtatgactgtccatactgaatataaaagaaatcaaatcattgctagttcagaaattgatgatcttatctttatgacgaaaccacaagagtggtcatttgaagagcaaaaagaaatacgggataaattagttcgtgaggctttttattttcactacaatagaaatgaagaatatagaaattattgtatcaatcagcatgtgagtgataatttacacactattgatgaaatacccgtgtttccaacatctgtttttaaatataagaaattacatactgtcacagccgaggacattgaaaattggtatacaagtagtggaactcgtggagtaaaaagtcatattgcacgtgatcgtcttagcattgaacgcttgcttggttctgtcaacttcggaatgaaatacgttggagattggtttgagcatcaaa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igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z