LOCUS       BBa_J10068              2641 bp    DNA     linear       22-Nov-2024
DEFINITION  luciferase + LRE + BD18 original RBS removed
ACCESSION   BBa_J10068
VERSION     BBa_J10068.1  
FEATURES             Location/Qualifiers
     misc_feature    1..17
                     /label=GGA prefix sticky word AATG
     misc_feature    15..17
                     /label=ATG from C-dog bicistron
     misc_feature    942..947
                     /label=double stop
     CDS             15..941
                     /label=luciferase generating enzyme
     misc_feature    971..973
                     /label=ATG
     CDS             971..2620
                     /label=green luciferase
     misc_feature    2615..2620
                     /label=double stop
     misc_feature    2621..2641
                     /label=BBa suffix
ORIGIN
        1 gaattcggtc tctaatggcc ccgaccgtgg aacaaattgt ggagctgggt acgtatctgc
       61 tggccgaaag cccgcactgg gacgatgaaa cgcaaagcct gtatttcgtc gatatcgttg
      121 gtcgcagcgt gaacaaatac gttccgacga ccaagacgca tacccaactg aaattcgaca
      181 agaatccgag cttcatcatc ccggtgaagg gttgttccga ccgttttatc gtcagcctgg
      241 aacgcgagat caacctgctg acctgggacg gcgcatcctc tgcaccatcc aagattgaga
      301 agatcgcggt tttcgataat acgccagaga agagcgaaaa ccgtctgaac gatggtaagg
      361 cagatccgct gggcaacctg tgggcgggta ccatgaatat gggttctgac cataccaccg
      421 gcacgccggt gcgcggtacc ctgagcagcc tgagcaacaa gcaagtcaaa gagcacgtca
      481 gcgaggtctg catcagcaat ggcctggcat ggagcaagga cctgaagaaa ttctattaca
      541 ttgatagcgc tgttcgtcaa gttgaccagt tcgatttcga tgcgaaaaac ctgagcttga
      601 gcaatcgtca accgctgttc acctttgaca aacacggcat catgggtagc ccggatggtc
      661 agaccatcga tgcggagggt aacctgtggg ttgccacctg tcagggcgat aaagtcctga
      721 agattgatac ttctaccccg gaaaccctgc tgggtattgt ggagattccg gaacatcagg
      781 tgacgagcgt ttgcatcggc ggtgcagaac tgaacgtttt gtatgtgacg accgcgtcca
      841 ttaaactgcc tggtgcggat gaaaccaagc cgatgaaagg cgccatctat aaagtcaccg
      901 gtctgggtgt caaaggtctg ccgggtgacc gtgttaagct gtaataagga tccttaagga
      961 ggtaaaaaaa atggagaaca tggagaacga cgagaatatt gtggttggtc cgaaaccgtt
     1021 ctacccgatc gaagagggca gcgcgggtac ccagctgcgt aaatacatgg aacgctacgc
     1081 gaaactgggt gcgatcgcgt tcacgaatgc ggttaccggc gtggactatt cctatgcgga
     1141 gtatttggaa aagtcgtgtt gtctgggcaa ggcgttacag aattatggcc tggtggtcga
     1201 tggccgtatc gcgctgtgca gcgagaactg tgaagagttt ttcattccgg tgatcgcggg
     1261 tttgtttatt ggcgtgggcg ttgcaccgac caatgaaatc tacacgctgc gcgaactggt
     1321 gcactccctg ggtatctcta agccgacgat cgttttcagc tccaagaaag gtttggataa
     1381 agttattacg gtgcaaaaga cggtcaccac gatcaaaacg attgtgatcc tggacagcaa
     1441 agttgattac cgcggttatc aatgcctgga tacgttcatc aagcgtaaca ccccgccagg
     1501 ttttcaagca tctagcttca aaacggtgga ggttgaccgt aaagaacaag tcgcactgat
     1561 tatgaatagc tcgggtagca cgggcctgcc aaagggtgtg cagctgaccc atgaaaacac
     1621 cgttacccgt tttagccacg cgcgtgatcc gatttacggc aatcaagtgt cgccgggtac
     1681 ggctattctg actgttgtcc cttttcacca tggttttggc atgtttacca ctctgggtta
     1741 cctgatttgc ggttttcgcg tcgttatgct gaccaagttt gatgaagaaa ccttcctgaa
     1801 aaccctgcaa gactacaagt gcaccagtgt gattctggtc ccgacgctgt tcgcgatcct
     1861 gaacaaaagc gagctgctga ataagtacga cctgagcaat ttggtggaga ttgcgagcgg
     1921 cggtgctccg ttgagcaaag aagtaggcga ggcagtggca cgtcgcttta acctgcctgg
     1981 cgtccgtcaa ggttacggtt tgactgaaac cacgagcgcg attatcatca ccccggaagg
     2041 tgacgataaa cctggcgcta gcggtaaggt cgtgccgttg ttcaaggcta aagttattga
     2101 tctggacacc aagaagagcc tgggcccgaa tcgccgtggt gaagtttgcg ttaagggccc
     2161 gatgctgatg aaaggttatg taaacaatcc ggaagcaacg aaagagctga tcgacgaaga
     2221 gggctggctg cacactggcg atatcggcta ctacgatgaa gagaaacact ttttcattgt
     2281 tgatcgcctg aaaagcctga tcaagtacaa gggttaccaa gtcccgccag cggagctgga
     2341 aagcgtactg ttgcagcacc cgtcgatttt cgacgccggt gtggctggtg tgcctgatcc
     2401 ggtggctggc gaactgccgg gtgctgtcgt ggtgctggag agcggtaaga atatgaccga
     2461 aaaagaagtc atggactatg tggcgagcca agtgtccaat gcaaaacgtc tgcgtggcgg
     2521 tgttcgcttt gtggacgaag tgccgaaagg tctgaccggc aaaattgacg gtcgtgcaat
     2581 tcgtgagatt ctgaagaaac cggtcgcgaa aatgtaataa tactagtagc ggccgctgca
     2641 g
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