LOCUS       BBa_J10069              2644 bp    DNA     linear       22-Nov-2024
DEFINITION  EPIC Firefly Luciferase and LRE  P. pyralis + BD18
ACCESSION   BBa_J10069
VERSION     BBa_J10069.1  
FEATURES             Location/Qualifiers
     misc_feature    1..17
                     /label=GGA prefix AATG sticky word
     misc_feature    15..17
                     /label=BD18 ATG
     CDS             15..944
                     /label=luciferin regenerating enzyme
     misc_feature    939..944
                     /label=double stop
     misc_feature    968..970
                     /label=ATG 
     CDS             968..2623
                     /label=EPIC luciferase
     misc_feature    2618..2623
                     /label=double stop
     misc_feature    2624..2644
                     /label=BBa suffix
ORIGIN
        1 gaattcggtc tctaatgggt ccggtcgttg aaaagatcgc cgagctgggt aagtacacgg
       61 ttggtgaggg tccgcactgg gaccacgaaa cgcagacgct gtatttcgtg gatacggtcg
      121 agaaaacgtt ccacaaatac gttccgagcc agaagaaata cacgttttgt aaggtggaca
      181 agctggttag ctttatcatt ccgctggcag gttctccggg tcgcttcgtc gtcagcttgg
      241 agcgcgagat tgcgatcctg acgtgggacg gcgtcagcgc ggcaccgacc agcattgagg
      301 cgattgtgaa tgtcgagccg cacatcaaga ataatcgcct gaacgacggt aaagcggacc
      361 cgctgggtaa tctgtggacc ggcacgatgg ccattgatgc gggtttgccg gtgggtccgg
      421 tgaccggctc cctgtaccac ctgggtgcgg ataagaaggt caagatgcat gaaagcaata
      481 ttgccatcgc taacggcctg gcgtggagca acgacctgaa gaagatgtac tatattgaca
      541 gcggcaaacg tcgtgttgac gaatacgact atgatgcgag cacgctgtct atcagcaatc
      601 agcgtccgct gttcaccttc gaaaaacatg aggtccctgg ctacccagac ggtcaaacca
      661 ttgacgaaga aggtaacctg tgggtcgccg tgtttcaagg ccagcgtatt atcaagatta
      721 gcacccagca gccggaggtt ctgctggata ccgtcaaaat cccggacccg caggtgacta
      781 gcgttgcatt cggtggtccg aatctggacg agctgcatgt gaccagcgcc ggtttgcagc
      841 tggatgacag ctccctggac aaatctttgg tcaatggtca cgtctaccgt gtgactggcc
      901 tgggtgttaa gggtttcgcc ggtgtgaaag ttaagctgta ataaggatcc ttaaggaggt
      961 aaaaaaaatg gaggacgcga aaaacatcaa gaaaggccca gcaccgttct atccgctgga
     1021 agatggtacc gccggtgaac aattgcacaa agcgatgaaa cgttatgcgc tggttccggg
     1081 taccatcgca ttcacggacg cgcatatcga ggttaacatc acctatgccg agtacttcga
     1141 gatgagcgtt cgtctggccg aggcaatgaa gcgttatggt ttgaatacca atcatcgcat
     1201 cgtcgtctgt agcgagaaca gcttgcagtt tttcatgcca gttctgggtg cactgtttat
     1261 tggtgtggca gtggcgcctg cgaatgatat ctacaacgag cgtgaactgt tgaatagcat
     1321 gaacatttcg cagccgacgg ttgtttttgt gtccaagaaa ggtttgcaga agattctgaa
     1381 tgtgcaaaag aagttgccga tcattcagaa aatcatcatc atggacagca agaccgatta
     1441 ccagggtttc caaagcatgt acacttttgt gacgagccac ctgcctccgg gttttaacga
     1501 gtatgatttc gttccggaaa gcttcgaccg tgataagacg attgcactga ttatgaactc
     1561 gtccggctct accggcctgc ctaaaggcgt ggccctgccg caccgtactg catgtgtccg
     1621 cttcagccac gcgcgtgatc cgatcttcgg taaccaaatc attccggaca ccgccatttt
     1681 gtctgtcgtt ccgtttcatc atggctttgg tatgtttacc actctgggtt acctgatctg
     1741 tggtttccgt gttgttctga tgtaccgttt cgaagaagaa ctgttcctgc gtagcctgca
     1801 agattacaag atccaatccg cgctgctggt gccgacgctg tttagcttct tcgctaaatc
     1861 caccctgatt gacaaatatg acttgagcaa cctgcacgaa atcgcgtcgg gcggtgcgcc
     1921 gctgagcaaa gaagtcggtg aggccgttgc gaaacgtttc catctgccgg gcatccgtca
     1981 gggttatggc ctgacggaaa cgaccagcgc gattttgatt acccctgagg gtgacgataa
     2041 accgggtgcc gttggcaagg tggtaccgtt ctttgaggct aaagtcgtgg acctggacac
     2101 gggtaagact ctgggtgtga atcaacgtgg cgagttgtgc gttcgtggcc cgatgatcat
     2161 gagcggttat gtcaacaacc cggaagcaac taatgcgttg atcgataagg atggctggct
     2221 gcacagcggc gacctggcgt actgggatga ggatgaacac tttttcatcg ttggtcgcct
     2281 gaagagcctg atcaagtaca agggctacca ggtggcaccg gctgaactgg agagcattct
     2341 gttgcaacac cctaatatct tcgacgcggg tgttgcgggt ctgcctgatg atgatgccgg
     2401 tgaattgcca gctgcggtgg tcgttctgga acacggtaaa acgatgactg aaaaagaaat
     2461 cgtggactac gtcgcctcgc aggtgaccac cgctaaaaag ttgcgtggtg gcgtggtgtt
     2521 tgtggatgaa gtgccgaaag gcctgacggg taaacgtgat gcacgtaaga ttcgtgagat
     2581 cttgatcaag gcgaagaaag gtggtaaatc caagttgtaa taatactagt agcggccgct
     2641 gcag
//