BBa_J10069 1 BBa_J10069 EPIC Firefly Luciferase and LRE P. pyralis + BD18 2012-01-10T12:00:00Z 2015-08-31T04:08:24Z from registry parts, synthesized de novo EPIC Firefly Luciferase and LRE from P. Pyralis (E. coli optimised) This is a modification of part K325100 originally built by iGEM10_Cambridge. We have removed the initial RBS to facilitate translation using C-dog bicistronic linker BD18 (J119024). This part is a translational unit for a mutant of the luciferase from the North American firefly (P. pyralis) as well as this species' luciferin regenerating enzyme (LRE). D-Luciferin has to be added to obtain light output. EPIC stands for Enhanced Photon Initiating Complex. It is described in Fujii et al. 2007 as having a 10 times higher substrate affinity and luminescence output compared to wildtype. The part is codon optimised for expression in E.coli. References [1]: S.M. Marques and J.C.G. Esteves da Silva, (2009) Firefly Bioluminescence: A Mechanistic Approach of Luciferase Catalyzed Reactions,Life 61, 6-17. [2]: T. Nakatsu et al. (2006) Structural Basis for the spectral difference in luciferase bioluminescence, Nature 440(16), 372-376. [3]: K. Gomi and N. Kajiyama, (2001) Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into Luciferin, The Journal of Biological Chemistry, 276(39), 36508-36513. false false _578_120_ 0 201 61 Not in stock false removed RBS from J119024 false Malcolm Campbell annotation2169605 1 luciferin regenerating enzyme range2169605 1 15 944 annotation2169604 1 BD18 ATG range2169604 1 15 17 annotation2169608 1 EPIC luciferase range2169608 1 968 2623 annotation2169607 1 ATG range2169607 1 968 970 annotation2169606 1 double stop range2169606 1 939 944 annotation2169610 1 BBa suffix range2169610 1 2624 2644 annotation2169603 1 GGA prefix AATG sticky word range2169603 1 1 17 annotation2169609 1 double stop range2169609 1 2618 2623 BBa_J10069_sequence 1 gaattcggtctctaatgggtccggtcgttgaaaagatcgccgagctgggtaagtacacggttggtgagggtccgcactgggaccacgaaacgcagacgctgtatttcgtggatacggtcgagaaaacgttccacaaatacgttccgagccagaagaaatacacgttttgtaaggtggacaagctggttagctttatcattccgctggcaggttctccgggtcgcttcgtcgtcagcttggagcgcgagattgcgatcctgacgtgggacggcgtcagcgcggcaccgaccagcattgaggcgattgtgaatgtcgagccgcacatcaagaataatcgcctgaacgacggtaaagcggacccgctgggtaatctgtggaccggcacgatggccattgatgcgggtttgccggtgggtccggtgaccggctccctgtaccacctgggtgcggataagaaggtcaagatgcatgaaagcaatattgccatcgctaacggcctggcgtggagcaacgacctgaagaagatgtactatattgacagcggcaaacgtcgtgttgacgaatacgactatgatgcgagcacgctgtctatcagcaatcagcgtccgctgttcaccttcgaaaaacatgaggtccctggctacccagacggtcaaaccattgacgaagaaggtaacctgtgggtcgccgtgtttcaaggccagcgtattatcaagattagcacccagcagccggaggttctgctggataccgtcaaaatcccggacccgcaggtgactagcgttgcattcggtggtccgaatctggacgagctgcatgtgaccagcgccggtttgcagctggatgacagctccctggacaaatctttggtcaatggtcacgtctaccgtgtgactggcctgggtgttaagggtttcgccggtgtgaaagttaagctgtaataaggatccttaaggaggtaaaaaaaatggaggacgcgaaaaacatcaagaaaggcccagcaccgttctatccgctggaagatggtaccgccggtgaacaattgcacaaagcgatgaaacgttatgcgctggttccgggtaccatcgcattcacggacgcgcatatcgaggttaacatcacctatgccgagtacttcgagatgagcgttcgtctggccgaggcaatgaagcgttatggtttgaataccaatcatcgcatcgtcgtctgtagcgagaacagcttgcagtttttcatgccagttctgggtgcactgtttattggtgtggcagtggcgcctgcgaatgatatctacaacgagcgtgaactgttgaatagcatgaacatttcgcagccgacggttgtttttgtgtccaagaaaggtttgcagaagattctgaatgtgcaaaagaagttgccgatcattcagaaaatcatcatcatggacagcaagaccgattaccagggtttccaaagcatgtacacttttgtgacgagccacctgcctccgggttttaacgagtatgatttcgttccggaaagcttcgaccgtgataagacgattgcactgattatgaactcgtccggctctaccggcctgcctaaaggcgtggccctgccgcaccgtactgcatgtgtccgcttcagccacgcgcgtgatccgatcttcggtaaccaaatcattccggacaccgccattttgtctgtcgttccgtttcatcatggctttggtatgtttaccactctgggttacctgatctgtggtttccgtgttgttctgatgtaccgtttcgaagaagaactgttcctgcgtagcctgcaagattacaagatccaatccgcgctgctggtgccgacgctgtttagcttcttcgctaaatccaccctgattgacaaatatgacttgagcaacctgcacgaaatcgcgtcgggcggtgcgccgctgagcaaagaagtcggtgaggccgttgcgaaacgtttccatctgccgggcatccgtcagggttatggcctgacggaaacgaccagcgcgattttgattacccctgagggtgacgataaaccgggtgccgttggcaaggtggtaccgttctttgaggctaaagtcgtggacctggacacgggtaagactctgggtgtgaatcaacgtggcgagttgtgcgttcgtggcccgatgatcatgagcggttatgtcaacaacccggaagcaactaatgcgttgatcgataaggatggctggctgcacagcggcgacctggcgtactgggatgaggatgaacactttttcatcgttggtcgcctgaagagcctgatcaagtacaagggctaccaggtggcaccggctgaactggagagcattctgttgcaacaccctaatatcttcgacgcgggtgttgcgggtctgcctgatgatgatgccggtgaattgccagctgcggtggtcgttctggaacacggtaaaacgatgactgaaaaagaaatcgtggactacgtcgcctcgcaggtgaccaccgctaaaaagttgcgtggtggcgtggtgtttgtggatgaagtgccgaaaggcctgacgggtaaacgtgatgcacgtaagattcgtgagatcttgatcaaggcgaagaaaggtggtaaatccaagttgtaataatactagtagcggccgctgcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z