LOCUS       BBa_J102005             2737 bp    DNA     linear       21-Sep-2024
DEFINITION  Biological Capacitor
ACCESSION   BBa_J102005
VERSION     BBa_J102005.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..721
                     /label=BBa_C0040
     stem_loop       766..809
                     /label=stem_loop
     misc_feature    755..834
                     /label=BBa_B0010
     terminator      755..834
                     /label=BBa_B0010
     stem_loop       850..869
                     /label=T7 TE
     misc_feature    876..876
                     /label=stop
     polyA_site      870..883
                     /label=polya
     misc_feature    843..883
                     /label=BBa_B0012
     terminator      843..883
                     /label=BBa_B0012
     misc_feature    892..910
                     /label=TetR 1
     misc_feature    892..945
                     /label=BBa_R0040
     promoter        911..916
                     /label=-35
     misc_feature    917..935
                     /label=TetR 2
     promoter        934..939
                     /label=-10
     promoter        892..945
                     /label=BBa_R0040
     misc_feature    44..945
                     /label=BBa_Q04400
     misc_feature    1..945
                     /label=BBa_J102002
     misc_feature    958..961
                     /label=conserved
     RBS             954..965
                     /label=BBa_B0034
     misc_feature    972..974
                     /label=start
     misc_feature    1725..1727
                     /label=stop
     misc_feature    1728..1730
                     /label=stop
     misc_feature    1688..1724
                     /label=LVA tag
     CDS             975..1687
                     /label=cds
     misc_feature    972..1730
                     /label=BBa_K145015
     misc_feature    954..1730
                     /label=BBa_J102000
     misc_feature    1..1730
                     /label=BBa_J102004
     promoter        1739..1793
                     /label=promoter
     promoter        1739..1793
                     /label=BBa_J102001
     misc_feature    1806..1809
                     /label=conserved
     RBS             1802..1813
                     /label=BBa_B0034
     misc_feature    1820..1821
                     /label=prefix
     misc_feature    1993..1993
                     /label=T
     misc_feature    2311..2311
                     /label=A
     CDS             1820..2569
                     /label=luxR
     misc_feature    1820..2575
                     /label=BBa_C0062
     misc_feature    2576..2600
                     /label=Help:Barcodes
     CDS             1820..2575
                     /label=BBa_C0062
     stem_loop       2620..2663
                     /label=stem_loop
     misc_feature    2609..2688
                     /label=BBa_B0010
     terminator      2609..2688
                     /label=BBa_B0010
     stem_loop       2704..2723
                     /label=T7 TE
     misc_feature    2730..2730
                     /label=stop
     polyA_site      2724..2737
                     /label=polya
     misc_feature    2697..2737
                     /label=BBa_B0012
     terminator      2697..2737
                     /label=BBa_B0012
     misc_feature    1802..2737
                     /label=BBa_I0462
     misc_feature    1739..2737
                     /label=BBa_J102003
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca
      901 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg
      961 agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
     1021 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
     1081 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
     1141 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc
     1201 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
     1261 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
     1321 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
     1381 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
     1441 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
     1501 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
     1561 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
     1621 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
     1681 acaaaaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa tactagagtt
     1741 tattcgacta taacaaacca ttttcttgcg taaacctgta cgatcctaca ggttactaga
     1801 gaaagaggag aaatactaga tgaaaaacat aaatgccgac gacacataca gaataattaa
     1861 taaaattaaa gcttgtagaa gcaataatga tattaatcaa tgcttatctg atatgactaa
     1921 aatggtacat tgtgaatatt atttactcgc gatcatttat cctcattcta tggttaaatc
     1981 tgatatttca atcctagata attaccctaa aaaatggagg caatattatg atgacgctaa
     2041 tttaataaaa tatgatccta tagtagatta ttctaactcc aatcattcac caattaattg
     2101 gaatatattt gaaaacaatg ctgtaaataa aaaatctcca aatgtaatta aagaagcgaa
     2161 aacatcaggt cttatcactg ggtttagttt ccctattcat acggctaaca atggcttcgg
     2221 aatgcttagt tttgcacatt cagaaaaaga caactatata gatagtttat ttttacatgc
     2281 gtgtatgaac ataccattaa ttgttccttc tctagttgat aattatcgaa aaataaatat
     2341 agcaaataat aaatcaaaca acgatttaac caaaagagaa aaagaatgtt tagcgtgggc
     2401 atgcgaagga aaaagctctt gggatatttc aaaaatatta ggttgcagtg agcgtactgt
     2461 cactttccat ttaaccaatg cgcaaatgaa actcaataca acaaaccgct gccaaagtat
     2521 ttctaaagca attttaacag gagcaattga ttgcccatac tttaaaaatt aataacactg
     2581 atagtgctag tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     2641 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     2701 actggctcac cttcgggtgg gcctttctgc gtttata
//