##gff-version 3
##sequence-region BBa_J102005 1 2737
BBa_J102005	.	engineered_region	1739	2737	.	+	0	ID=BBa_J102003;Name=BBa_J102003
BBa_J102005	.	engineered_region	1802	2737	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J102003
BBa_J102005	.	terminator	2609	2688	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_J102005	.	stem_loop	2620	2663	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J102005	.	engineered_region	2609	2688	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J102005	.	ribosome_entry_site	1802	1813	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_J102005	.	conserved_region	1806	1809	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J102005	.	terminator	2697	2737	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_J102005	.	engineered_region	2697	2737	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J102005	.	stop_codon	2730	2730	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J102005	.	polyA_site	2724	2737	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J102005	.	stem_loop	2704	2723	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J102005	.	CDS	1820	2575	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_J102005	.	point_mutation	2311	2311	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J102005	.	engineered_region	1820	2575	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J102005	.	engineered_region	1820	1821	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J102005	.	CDS	1820	2569	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J102005	.	point_mutation	1993	1993	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J102005	.	engineered_tag	2576	2600	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J102005	.	promoter	1739	1793	.	+	0	ID=BBa_J102001;Name=BBa_J102001;Parent=BBa_J102003
BBa_J102005	.	promoter	1739	1793	.	+	0	ID=annotation2072374;Name=promoter;Parent=BBa_J102001
BBa_J102005	.	engineered_region	1	1730	.	+	0	ID=BBa_J102004;Name=BBa_J102004
BBa_J102005	.	engineered_region	1	945	.	+	0	ID=BBa_J102002;Name=BBa_J102002;Parent=BBa_J102004
BBa_J102005	.	promoter	1	35	.	+	0	ID=BBa_J23119;Name=BBa_J23119;Parent=BBa_J102002
BBa_J102005	.	engineered_region	44	945	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400;Parent=BBa_J102002
BBa_J102005	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_J102005	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J102005	.	CDS	62	721	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_J102005	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J102005	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J102005	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J102005	.	promoter	892	945	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_J102005	.	promoter	911	916	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J102005	.	non_covalent_binding_site	917	935	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J102005	.	engineered_region	892	945	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J102005	.	non_covalent_binding_site	892	910	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J102005	.	promoter	934	939	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J102005	.	terminator	843	883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_J102005	.	engineered_region	843	883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J102005	.	stop_codon	876	876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J102005	.	polyA_site	870	883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J102005	.	stem_loop	850	869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J102005	.	terminator	755	834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_J102005	.	stem_loop	766	809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J102005	.	engineered_region	755	834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J102005	.	engineered_region	954	1730	.	+	0	ID=BBa_J102000;Name=BBa_J102000;Parent=BBa_J102004
BBa_J102005	.	ribosome_entry_site	954	965	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J102000
BBa_J102005	.	conserved_region	958	961	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J102005	.	engineered_region	972	1730	.	+	0	ID=BBa_K145015;Name=BBa_K145015;Parent=BBa_J102000
BBa_J102005	.	tag	1688	1724	.	+	0	ID=annotation1969619;Name=LVA tag;Parent=BBa_K145015
BBa_J102005	.	stop_codon	1725	1727	.	+	0	ID=annotation1969617;Name=stop;Parent=BBa_K145015
BBa_J102005	.	stop_codon	1728	1730	.	+	0	ID=annotation1969618;Name=stop;Parent=BBa_K145015
BBa_J102005	.	start_codon	972	974	.	+	0	ID=annotation1969616;Name=start;Parent=BBa_K145015
BBa_J102005	.	CDS	975	1687	.	+	0	ID=annotation1969620;Name=cds;Parent=BBa_K145015
>BBa_J102005
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactac
tagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattaga
tggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaa
tttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttg
cgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactat
atttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgag
ttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtat
acatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttca
actagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccaca
caatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggatta
cacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagag
tttattcgactataacaaaccattttcttgcgtaaacctgtacgatcctacaggttactagagaaagaggagaaatact
agatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaa
tcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtt
aaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatg
atcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaa
atctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaat
ggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaaca
taccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaac
caaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagt
gagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaag
caattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata