LOCUS       BBa_J107014             2722 bp    DNA     linear       19-Nov-2024
DEFINITION  Heat-inducible lysis device
ACCESSION   BBa_J107014
VERSION     BBa_J107014.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23114
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             56..769
                     /label=cIts
     CDS             56..769
                     /label=BBa_K098997
     misc_feature    44..769
                     /label=BBa_K098998
     stem_loop       785..804
                     /label=T7 TE
     misc_feature    811..811
                     /label=stop
     polyA_site      805..818
                     /label=polya
     misc_feature    778..818
                     /label=BBa_B0012
     terminator      778..818
                     /label=BBa_B0012
     stem_loop       839..861
                     /label=stem_loop
     misc_feature    827..872
                     /label=BBa_B0011
     terminator      827..872
                     /label=BBa_B0011
     promoter        918..923
                     /label=-10
     promoter        895..900
                     /label=-35
     misc_feature    905..921
                     /label=OR1
     misc_feature    881..897
                     /label=OR2
     misc_feature    881..929
                     /label=BBa_R0051
     promoter        881..929
                     /label=BBa_R0051
     misc_feature    778..929
                     /label=BBa_S04136
     misc_feature    44..929
                     /label=BBa_S04137
     misc_feature    1..929
                     /label=BBa_K098995
     CDS             954..1610
                     /label=T4 holin
     CDS             938..1613
                     /label=BBa_K112805
     CDS             1638..2132
                     /label=T4 Endolysin
     CDS             1622..2135
                     /label=BBa_K112806
     stem_loop       2155..2198
                     /label=stem_loop
     misc_feature    2144..2223
                     /label=BBa_B0010
     terminator      2144..2223
                     /label=BBa_B0010
     stem_loop       2239..2258
                     /label=T7 TE
     misc_feature    2265..2265
                     /label=stop
     polyA_site      2259..2272
                     /label=polya
     misc_feature    2232..2272
                     /label=BBa_B0012
     terminator      2232..2272
                     /label=BBa_B0012
     promoter        2281..2315
                     /label=BBa_J23116
     CDS             2338..2631
                     /label=T4 antiholin
     CDS             2324..2634
                     /label=BBa_K112807
     stem_loop       2654..2697
                     /label=stem_loop
     misc_feature    2643..2722
                     /label=BBa_B0010
     terminator      2643..2722
                     /label=BBa_B0010
     misc_feature    938..2722
                     /label=BBa_K112808
ORIGIN
        1 tttatggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaaatgag
       61 cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat
      121 ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg
      181 gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa
      241 cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc
      301 cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta
      361 tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctgagc ttagaacctt
      421 taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt
      481 ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc
      541 tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat
      601 agccagactt gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt
      661 gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt
      721 tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggctgat actagagtca
      781 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa
      841 gccagattat taatccggct tttttattat tttactagag taacaccgtg cgtgttgact
      901 attttacctc tggcggtgat aatggttgct actagagctt aaaaggaggg tctatggcag
      961 cacctagaat atcattttcg ccctctgata ttctatttgg tgttctcgat cgcttgttca
     1021 aagataacgc taccgggaag gttcttgctt cccgggtagc tgtcgtaatt cttttgttta
     1081 taatggcgat tgtttggtat aggggagata gtttctttga gtactataag caatcaaagt
     1141 atgaaacata cagtgaaatt attgaaaagg aaagaactgc acgctttgaa tctgtcgccc
     1201 tggaacaact ccagatagtt catatatcat ctgaggcaga ctttagtgcg gtgtattctt
     1261 tccgccctaa aaacttaaac tattttgttg atattatagc atacgaagga aaattacctt
     1321 caacaataag tgaaaaatca cttggaggat atcctgttga taaaactatg gatgaatata
     1381 cagttcattt aaatggacgt cattattatt ccaactcaaa atttgctttt ttaccaacta
     1441 aaaagcctac tcccgaaata aactacatgt acagttgtcc atattttaat ttggataata
     1501 tctatgctgg aacgataacc atgtactggt atagaaatga tcatataagt aatgaccgcc
     1561 ttgaatcaat atgtgctcag gcggccagaa tattaggaag ggctaaataa ttatactaga
     1621 gatacttagg aggtattatg aatatatttg aaatgttacg tatagatgaa ggtcttagac
     1681 ttaaaatcta taaagacaca gaaggctatt acactattgg catcggtcat ttgcttacaa
     1741 aaagtccatc acttaatgct gctaaatctg aattagataa agctattggg cgtaattgca
     1801 atggtgtaat tacaaaagat gaggctgaaa aactctttaa tcaggatgtt gatgctgctg
     1861 ttcgcggaat cctgagaaat gctaaattaa aaccggttta tgattctctt gatgcggttc
     1921 gtcgctgtgc attgattaat atggttttcc aaatgggaga aaccggtgtg gcaggattta
     1981 ctaactcttt acgtatgctt caacaaaaac gctgggatga agcagcagtt aacttagcta
     2041 aaagtagatg gtataatcaa acacctaatc gcgcaaaacg agtcattaca acgtttagaa
     2101 ctggcacttg ggacgcgtat aaaaatctat aaagctacta gagccaggca tcaaataaaa
     2161 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2221 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag
     2281 ttgacagcta gctcagtcct agggactatg ctagctacta gaggatagga ggcctttatg
     2341 gccttaaaag caacagcact ttttgccatg ctaggattgt catttgtttt atctccatcg
     2401 attgaagcga atgtcgatcc tcattttgat aaatttatgg aatctggtat taggcacgtt
     2461 tatatgcttt ttgaaaataa aagcgtagaa tcgtctgaac aattctatag ttttatgaga
     2521 acgacctata aaaatgaccc gtgctcttct gattttgaat gtatagagcg aggcgcggag
     2581 atggcacaat catacgctag aattatgaac attaaattgg agactgaatg aaattactag
     2641 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     2701 ttgtttgtcg gtgaacgctc tc
//