LOCUS       BBa_J107015             1845 bp    DNA     linear       22-Nov-2024
DEFINITION  Constitutive generator of GFP (J23118) and RFP (R0051)
ACCESSION   BBa_J107015
VERSION     BBa_J107015.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23118
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             63..782
                     /label=GFP protein
     CDS             63..782
                     /label=BBa_E0040
     stem_loop       802..845
                     /label=stem_loop
     misc_feature    791..870
                     /label=BBa_B0010
     terminator      791..870
                     /label=BBa_B0010
     stem_loop       886..905
                     /label=T7 TE
     misc_feature    912..912
                     /label=stop
     polyA_site      906..919
                     /label=polya
     misc_feature    879..919
                     /label=BBa_B0012
     terminator      879..919
                     /label=BBa_B0012
     misc_feature    1..919
                     /label=BBa_K173002
     promoter        965..970
                     /label=-10
     promoter        942..947
                     /label=-35
     misc_feature    952..968
                     /label=OR1
     misc_feature    928..944
                     /label=OR2
     misc_feature    928..976
                     /label=BBa_R0051
     promoter        928..976
                     /label=BBa_R0051
     misc_feature    989..992
                     /label=conserved
     RBS             985..996
                     /label=BBa_B0034
     CDS             1003..1677
                     /label=mrfp1
     misc_feature    1684..1708
                     /label=Help:Barcodes
     CDS             1003..1683
                     /label=BBa_E1010
     stem_loop       1728..1771
                     /label=stem_loop
     misc_feature    1717..1796
                     /label=BBa_B0010
     terminator      1717..1796
                     /label=BBa_B0010
     stem_loop       1812..1831
                     /label=T7 TE
     misc_feature    1838..1838
                     /label=stop
     polyA_site      1832..1845
                     /label=polya
     misc_feature    1805..1845
                     /label=BBa_B0012
     terminator      1805..1845
                     /label=BBa_B0012
     misc_feature    985..1845
                     /label=BBa_I13507
     misc_feature    928..1845
                     /label=BBa_I763007
ORIGIN
        1 ttgacggcta gctcagtcct aggtattgtg ctagctacta gagtcacaca ggaaagtact
       61 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      121 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      181 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      241 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
      301 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
      361 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
      421 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
      481 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
      541 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
      601 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
      661 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
      721 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
      781 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      841 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      901 gggcctttct gcgtttatat actagagtaa caccgtgcgt gttgactatt ttacctctgg
      961 cggtgataat ggttgctact agagaaagag gagaaatact agatggcttc ctccgaagac
     1021 gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag
     1081 ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg
     1141 aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag
     1201 tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc
     1261 ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc
     1321 gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt
     1381 accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc
     1441 accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa
     1501 ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg
     1561 gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa
     1621 gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa
     1681 taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg
     1741 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1801 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//