LOCUS       BBa_J107017             1833 bp    DNA     linear       22-Nov-2024
DEFINITION  Constitutive generator of GFP (J23118) and RFP (I14032)
ACCESSION   BBa_J107017
VERSION     BBa_J107017.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23118
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             63..782
                     /label=GFP protein
     CDS             63..782
                     /label=BBa_E0040
     stem_loop       802..845
                     /label=stem_loop
     misc_feature    791..870
                     /label=BBa_B0010
     terminator      791..870
                     /label=BBa_B0010
     stem_loop       886..905
                     /label=T7 TE
     misc_feature    912..912
                     /label=stop
     polyA_site      906..919
                     /label=polya
     misc_feature    879..919
                     /label=BBa_B0012
     terminator      879..919
                     /label=BBa_B0012
     misc_feature    1..919
                     /label=BBa_K173002
     misc_feature    928..964
                     /label=P(Lac) IQ
     promoter        930..935
                     /label=-35
     promoter        953..958
                     /label=-10
     promoter        928..964
                     /label=BBa_I14032
     misc_feature    977..980
                     /label=conserved
     RBS             973..984
                     /label=BBa_B0034
     CDS             991..1665
                     /label=mrfp1
     misc_feature    1672..1696
                     /label=Help:Barcodes
     CDS             991..1671
                     /label=BBa_E1010
     stem_loop       1716..1759
                     /label=stem_loop
     misc_feature    1705..1784
                     /label=BBa_B0010
     terminator      1705..1784
                     /label=BBa_B0010
     stem_loop       1800..1819
                     /label=T7 TE
     misc_feature    1826..1826
                     /label=stop
     polyA_site      1820..1833
                     /label=polya
     misc_feature    1793..1833
                     /label=BBa_B0012
     terminator      1793..1833
                     /label=BBa_B0012
     misc_feature    973..1833
                     /label=BBa_I13507
     misc_feature    928..1833
                     /label=BBa_J107012
ORIGIN
        1 ttgacggcta gctcagtcct aggtattgtg ctagctacta gagtcacaca ggaaagtact
       61 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      121 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      181 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      241 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
      301 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
      361 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
      421 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
      481 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
      541 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
      601 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
      661 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
      721 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
      781 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      841 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      901 gggcctttct gcgtttatat actagagtgg tgcaaaacct ttcgcggtat ggcatgatag
      961 cgcctactag agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag
     1021 ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa
     1081 ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa
     1141 ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa
     1201 gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt
     1261 ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac
     1321 tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg
     1381 tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg
     1441 tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt
     1501 ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg
     1561 ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc
     1621 gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag
     1681 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1741 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1801 gctcaccttc gggtgggcct ttctgcgttt ata
//