LOCUS       BBa_J107018             1833 bp    DNA     linear       01-Jun-2024
DEFINITION  Constitutive generator of RFP (I14032) and GFP (J23118)
ACCESSION   BBa_J107018
VERSION     BBa_J107018.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     misc_feature    50..53
                     /label=conserved
     RBS             46..57
                     /label=BBa_B0034
     CDS             64..738
                     /label=mrfp1
     misc_feature    745..769
                     /label=Help:Barcodes
     CDS             64..744
                     /label=BBa_E1010
     stem_loop       789..832
                     /label=stem_loop
     misc_feature    778..857
                     /label=BBa_B0010
     terminator      778..857
                     /label=BBa_B0010
     stem_loop       873..892
                     /label=T7 TE
     misc_feature    899..899
                     /label=stop
     polyA_site      893..906
                     /label=polya
     misc_feature    866..906
                     /label=BBa_B0012
     terminator      866..906
                     /label=BBa_B0012
     misc_feature    46..906
                     /label=BBa_I13507
     misc_feature    1..906
                     /label=BBa_J107012
     promoter        915..949
                     /label=BBa_J23118
     RBS             958..970
                     /label=RBS-3\Weak
     RBS             964..967
                     /label=RBS
     misc_feature    958..970
                     /label=BBa_B0032
     RBS             958..970
                     /label=BBa_B0032
     CDS             977..1696
                     /label=GFP protein
     CDS             977..1696
                     /label=BBa_E0040
     stem_loop       1716..1759
                     /label=stem_loop
     misc_feature    1705..1784
                     /label=BBa_B0010
     terminator      1705..1784
                     /label=BBa_B0010
     stem_loop       1800..1819
                     /label=T7 TE
     misc_feature    1826..1826
                     /label=stop
     polyA_site      1820..1833
                     /label=polya
     misc_feature    1793..1833
                     /label=BBa_B0012
     terminator      1793..1833
                     /label=BBa_B0012
     misc_feature    915..1833
                     /label=BBa_K173002
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac
       61 tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa
      121 ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa
      181 ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac
      241 atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc
      301 ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc
      361 gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc
      421 tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa
      481 accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt
      541 gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc
      601 acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg
      661 gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt
      721 cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct actagagcca
      781 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      841 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      901 tttatatact agagttgacg gctagctcag tcctaggtat tgtgctagct actagagtca
      961 cacaggaaag tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct
     1021 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
     1081 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
     1141 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc
     1201 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
     1261 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
     1321 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
     1381 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
     1441 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
     1501 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     1561 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
     1621 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
     1681 actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1741 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1801 gctcaccttc gggtgggcct ttctgcgttt ata
//