##gff-version 3
##sequence-region BBa_J107018 1 1833
BBa_J107018	.	engineered_region	1	906	.	+	0	ID=BBa_J107012;Name=BBa_J107012
BBa_J107018	.	engineered_region	46	906	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_J107012
BBa_J107018	.	terminator	866	906	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_J107018	.	stem_loop	873	892	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J107018	.	stop_codon	899	899	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J107018	.	polyA_site	893	906	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J107018	.	engineered_region	866	906	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J107018	.	ribosome_entry_site	46	57	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_J107018	.	conserved_region	50	53	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J107018	.	CDS	64	744	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_J107018	.	engineered_tag	745	769	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J107018	.	CDS	64	738	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J107018	.	terminator	778	857	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_J107018	.	stem_loop	789	832	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J107018	.	engineered_region	778	857	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J107018	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_J107012
BBa_J107018	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_J107018	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_J107018	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_J107018	.	engineered_region	915	1833	.	+	0	ID=BBa_K173002;Name=BBa_K173002
BBa_J107018	.	CDS	977	1696	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K173002
BBa_J107018	.	CDS	977	1696	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J107018	.	ribosome_entry_site	958	970	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K173002
BBa_J107018	.	ribosome_entry_site	958	970	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J107018	.	engineered_region	958	970	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J107018	.	ribosome_entry_site	964	967	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J107018	.	terminator	1705	1784	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K173002
BBa_J107018	.	stem_loop	1716	1759	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J107018	.	engineered_region	1705	1784	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J107018	.	terminator	1793	1833	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K173002
BBa_J107018	.	stem_loop	1800	1819	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J107018	.	stop_codon	1826	1826	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J107018	.	polyA_site	1820	1833	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J107018	.	engineered_region	1793	1833	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J107018	.	promoter	915	949	.	+	0	ID=BBa_J23118;Name=BBa_J23118;Parent=BBa_K173002
>BBa_J107018
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatggcttcctccgaag
acgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtga
aggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgg
gacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaac
tgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactc
ctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcag
aaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgc
gtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgcc
gggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgt
gctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtattgtgctag
ctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaa
ttagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccc
ttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatg
ctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaaga
actatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaa
tcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaa
tgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagc
gttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgt
ccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgg
gattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttata