LOCUS       BBa_J107024             1920 bp    DNA     linear       25-Nov-2024
DEFINITION  3OC6HSL-repressible device with GFP
ACCESSION   BBa_J107024
VERSION     BBa_J107024.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     misc_feature    1..998
                     /label=BBa_S03119
     promoter        1007..1012
                     /label=-35
     promoter        1031..1036
                     /label=-10
     misc_feature    1012..1031
                     /label=Lux Box
     promoter        1007..1036
                     /label=BBa_R0061
     RBS             1045..1057
                     /label=RBS-3\Weak
     RBS             1051..1054
                     /label=RBS
     misc_feature    1045..1057
                     /label=BBa_B0032
     RBS             1045..1057
                     /label=BBa_B0032
     CDS             1064..1783
                     /label=GFP protein
     CDS             1064..1783
                     /label=BBa_E0040
     stem_loop       1803..1846
                     /label=stem_loop
     misc_feature    1792..1871
                     /label=BBa_B0010
     terminator      1792..1871
                     /label=BBa_B0010
     stem_loop       1887..1906
                     /label=T7 TE
     misc_feature    1913..1913
                     /label=stop
     polyA_site      1907..1920
                     /label=polya
     misc_feature    1880..1920
                     /label=BBa_B0012
     terminator      1880..1920
                     /label=BBa_B0012
     misc_feature    1045..1920
                     /label=BBa_E0240
     misc_feature    1007..1920
                     /label=BBa_J107022
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagttga cacctgtagg
     1021 atcgtacagg tataattact agagtcacac aggaaagtac tagatgcgta aaggagaaga
     1081 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
     1141 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
     1201 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta
     1261 tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag
     1321 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta
     1381 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa
     1441 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa
     1501 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa
     1561 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
     1621 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
     1681 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
     1741 tgctgggatt acacatggca tggatgaact atacaaataa taatactaga gccaggcatc
     1801 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1861 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//