##gff-version 3
##sequence-region BBa_J107025 1 1905
BBa_J107025	.	engineered_region	1	998	.	+	0	ID=BBa_S03119;Name=BBa_S03119
BBa_J107025	.	terminator	958	998	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03119
BBa_J107025	.	engineered_region	958	998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J107025	.	stop_codon	991	991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J107025	.	polyA_site	985	998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J107025	.	stem_loop	965	984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J107025	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_S03119
BBa_J107025	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J107025	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J107025	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J107025	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J107025	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J107025	.	terminator	870	949	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03119
BBa_J107025	.	stem_loop	881	924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J107025	.	engineered_region	870	949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J107025	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03119
BBa_J107025	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J107025	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S03119
BBa_J107025	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J107025	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J107025	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J107025	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J107025	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J107025	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J107025	.	engineered_region	1007	1905	.	+	0	ID=BBa_J107023;Name=BBa_J107023
BBa_J107025	.	engineered_region	1045	1905	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_J107023
BBa_J107025	.	ribosome_entry_site	1045	1056	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_J107025	.	conserved_region	1049	1052	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J107025	.	terminator	1777	1856	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_J107025	.	stem_loop	1788	1831	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J107025	.	engineered_region	1777	1856	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J107025	.	terminator	1865	1905	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_J107025	.	engineered_region	1865	1905	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J107025	.	stop_codon	1898	1898	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J107025	.	polyA_site	1892	1905	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J107025	.	stem_loop	1872	1891	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J107025	.	CDS	1063	1743	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_J107025	.	CDS	1063	1737	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J107025	.	engineered_tag	1744	1768	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J107025	.	promoter	1007	1036	.	+	0	ID=BBa_R0061;Name=BBa_R0061;Parent=BBa_J107023
BBa_J107025	.	non_covalent_binding_site	1012	1031	.	+	0	ID=annotation305877;Name=Lux Box;Parent=BBa_R0061
BBa_J107025	.	promoter	1007	1012	.	+	0	ID=annotation305863;Name=-35;Parent=BBa_R0061
BBa_J107025	.	promoter	1031	1036	.	+	0	ID=annotation305864;Name=-10;Parent=BBa_R0061
>BBa_J107025
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacacctgtaggatcgtac
aggtataattactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaa
agttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacc
cagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacg
gttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatggga
acgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctac
aaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttcca
ccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcacta
cgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactg
gacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctt
aataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttata