LOCUS BBa_J107026 2852 bp DNA linear 15-Nov-2024 DEFINITION 3OC6HSL-dependent positive/negative switch ACCESSION BBa_J107026 VERSION BBa_J107026.1 FEATURES Location/Qualifiers promoter 1..6 /label=-35 promoter 25..30 /label=-10 misc_feature 6..25 /label=Lux Box promoter 1..30 /label=BBa_R0061 misc_feature 43..46 /label=conserved RBS 39..50 /label=BBa_B0034 CDS 57..731 /label=mrfp1 misc_feature 738..762 /label=Help:Barcodes CDS 57..737 /label=BBa_E1010 stem_loop 782..825 /label=stem_loop misc_feature 771..850 /label=BBa_B0010 terminator 771..850 /label=BBa_B0010 stem_loop 866..885 /label=T7 TE misc_feature 892..892 /label=stop polyA_site 886..899 /label=polya misc_feature 859..899 /label=BBa_B0012 terminator 859..899 /label=BBa_B0012 misc_feature 39..899 /label=BBa_I13507 misc_feature 1..899 /label=BBa_J107023 misc_feature 908..926 /label=TetR 1 misc_feature 908..961 /label=BBa_R0040 promoter 927..932 /label=-35 misc_feature 933..951 /label=TetR 2 promoter 950..955 /label=-10 promoter 908..961 /label=BBa_R0040 misc_feature 974..977 /label=conserved RBS 970..981 /label=BBa_B0034 misc_feature 988..989 /label=prefix misc_feature 1161..1161 /label=T misc_feature 1479..1479 /label=A CDS 988..1737 /label=luxR misc_feature 988..1743 /label=BBa_C0062 misc_feature 1744..1768 /label=Help:Barcodes CDS 988..1743 /label=BBa_C0062 stem_loop 1788..1831 /label=stem_loop misc_feature 1777..1856 /label=BBa_B0010 terminator 1777..1856 /label=BBa_B0010 stem_loop 1872..1891 /label=T7 TE misc_feature 1898..1898 /label=stop polyA_site 1892..1905 /label=polya misc_feature 1865..1905 /label=BBa_B0012 terminator 1865..1905 /label=BBa_B0012 misc_feature 1914..1933 /label=LuxR/HSL promoter 1933..1938 /label=-35 promoter 1955..1960 /label=-10 misc_feature 1966..1966 /label=start misc_feature 1914..1968 /label=BBa_R0062 promoter 1914..1968 /label=BBa_R0062 RBS 1977..1989 /label=RBS-3\Weak RBS 1983..1986 /label=RBS misc_feature 1977..1989 /label=BBa_B0032 RBS 1977..1989 /label=BBa_B0032 CDS 1996..2715 /label=GFP protein CDS 1996..2715 /label=BBa_E0040 stem_loop 2735..2778 /label=stem_loop misc_feature 2724..2803 /label=BBa_B0010 terminator 2724..2803 /label=BBa_B0010 stem_loop 2819..2838 /label=T7 TE misc_feature 2845..2845 /label=stop polyA_site 2839..2852 /label=polya misc_feature 2812..2852 /label=BBa_B0012 terminator 2812..2852 /label=BBa_B0012 misc_feature 908..2852 /label=BBa_T9002 ORIGIN 1 ttgacacctg taggatcgta caggtataat tactagagaa agaggagaaa tactagatgg 61 cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg 121 ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc 181 agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt 241 ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact 301 acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg 361 gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag 421 ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg 481 gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca 541 aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca 601 tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca 661 cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact 721 ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca 781 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 841 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat 901 actagagtcc ctatcagtga tagagattga catccctatc agtgatagag atactgagca 961 ctactagaga aagaggagaa atactagatg aaaaacataa atgccgacga cacatacaga 1021 ataattaata aaattaaagc ttgtagaagc aataatgata ttaatcaatg cttatctgat 1081 atgactaaaa tggtacattg tgaatattat ttactcgcga tcatttatcc tcattctatg 1141 gttaaatctg atatttcaat cctagataat taccctaaaa aatggaggca atattatgat 1201 gacgctaatt taataaaata tgatcctata gtagattatt ctaactccaa tcattcacca 1261 attaattgga atatatttga aaacaatgct gtaaataaaa aatctccaaa tgtaattaaa 1321 gaagcgaaaa catcaggtct tatcactggg tttagtttcc ctattcatac ggctaacaat 1381 ggcttcggaa tgcttagttt tgcacattca gaaaaagaca actatataga tagtttattt 1441 ttacatgcgt gtatgaacat accattaatt gttccttctc tagttgataa ttatcgaaaa 1501 ataaatatag caaataataa atcaaacaac gatttaacca aaagagaaaa agaatgttta 1561 gcgtgggcat gcgaaggaaa aagctcttgg gatatttcaa aaatattagg ttgcagtgag 1621 cgtactgtca ctttccattt aaccaatgcg caaatgaaac tcaatacaac aaaccgctgc 1681 caaagtattt ctaaagcaat tttaacagga gcaattgatt gcccatactt taaaaattaa 1741 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg 1801 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1861 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagacctgta 1921 ggatcgtaca ggtttacgca agaaaatggt ttgttatagt cgaataaata ctagagtcac 1981 acaggaaagt actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt 2041 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt 2101 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt 2161 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca 2221 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa 2281 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa 2341 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac 2401 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac 2461 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc 2521 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta 2581 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga 2641 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa 2701 ctatacaaat aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 2761 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 2821 ctcaccttcg ggtgggcctt tctgcgttta ta //