LOCUS       BBa_J107030              904 bp    DNA     linear       26-Dec-2024
DEFINITION  tetR-based QPI with weak RBS
ACCESSION   BBa_J107030
VERSION     BBa_J107030.1  
FEATURES             Location/Qualifiers
     misc_feature    7..10
                     /label=conserved
     RBS             1..14
                     /label=BBa_B0031
     CDS             24..640
                     /label=tetR
     misc_feature    641..674
                     /label=SsrA
     misc_feature    681..705
                     /label=Help:Barcodes
     CDS             21..680
                     /label=BBa_C0040
     stem_loop       725..768
                     /label=stem_loop
     misc_feature    714..793
                     /label=BBa_B0010
     terminator      714..793
                     /label=BBa_B0010
     stem_loop       809..828
                     /label=T7 TE
     misc_feature    835..835
                     /label=stop
     polyA_site      829..842
                     /label=polya
     misc_feature    802..842
                     /label=BBa_B0012
     terminator      802..842
                     /label=BBa_B0012
     misc_feature    1..842
                     /label=BBa_P0140
     misc_feature    851..869
                     /label=TetR 1
     misc_feature    851..904
                     /label=BBa_R0040
     promoter        870..875
                     /label=-35
     misc_feature    876..894
                     /label=TetR 2
     promoter        893..898
                     /label=-10
     promoter        851..904
                     /label=BBa_R0040
ORIGIN
        1 tcacacagga aacctactag atgtccagat tagataaaag taaagtgatt aacagcgcat
       61 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
      121 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
      181 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
      241 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
      301 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
      361 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
      421 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
      481 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
      541 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
      601 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact
      661 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagccaggca
      721 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      781 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      841 tatactagag tccctatcag tgatagagat tgacatccct atcagtgata gagatactga
      901 gcac
//