BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_G0002
1
SX scar
SpeI/XbaI mixed site
2007-02-26T12:00:00Z
2015-08-31T04:07:27Z
XbaI and SpeI sites
XbaI/SpeI mixed site. Simply used to aid in entry of parts into the registry.
false
true
_41_
0
126
70
Not in stock
false
None.
false
Reshma Shetty
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation7019
1
BBa_B0011
range7019
1
1
46
annotation1683
1
stem_loop
range1683
1
13
35
BBa_R0051
1
cI lam
promoter (lambda cI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
<a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
Released HQ 2013
The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (<b><font color="red">More details needed here! DE, 2/24/03</font></b>)<P> Incompatible with host expressing cI repressor.
true
Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
annotation2023
1
-35
range2023
1
15
20
annotation2022
1
-10
range2022
1
38
43
annotation7067
1
BBa_R0051
range7067
1
1
49
annotation2025
1
OR2
range2025
1
1
17
annotation2024
1
OR1
range2024
1
25
41
BBa_J107033
1
BBa_J107033
cI temperature sensitive
2013-07-14T11:00:00Z
2015-08-31T04:08:25Z
CRIM helper plasmid pAH123, complete sequence.
Coding sequence of the cIts gene. It has two point mutations compared to the common lambda cI gene (<partinfo>BBa_C0051</partinfo>) that confer the heat-sensitive behaviour.
false
false
_587_
0
2621
9
Not in stock
false
-
false
Lorenzo Pasotti
BBa_J107034
1
BBa_J107034
heat-inducible device
2013-07-14T11:00:00Z
2015-08-31T04:08:25Z
This device is identical to <partinfo>BBa_K098995</partinfo>, but contains the correct sequence of cIts. It has been submitted as a new part to fix sequence bugs.
cIts/PR-based heat-inducible device. The weak promoter J23114 drives the expression of cIts. At low temperature (<37??C) cIts represses PR (OFF state), while at high temperature cIts is inhibited and PR transcription is ON. For these reasons, this device can generate a transcriptional signal in a temperature-dependent fashion.
false
false
_587_
0
2621
9
Not in stock
false
same as <partinfo>BBa_K098995</partinfo>
false
Lorenzo Pasotti
component2330618
1
BBa_G0002
component2330632
1
BBa_R0051
component2330627
1
BBa_G0002
component2330620
1
BBa_B0034
component2330623
1
BBa_B0012
component2330628
1
BBa_B0011
component2330621
1
BBa_J107033
component2330622
1
BBa_G0002
component2330630
1
BBa_G0002
component2330617
1
BBa_J23114
annotation2330622
1
BBa_G0002
range2330622
1
770
777
annotation2330632
1
BBa_R0051
range2330632
1
881
929
annotation2330620
1
BBa_B0034
range2330620
1
44
55
annotation2330618
1
BBa_G0002
range2330618
1
36
43
annotation2330621
1
BBa_J107033
range2330621
1
56
769
annotation2330617
1
BBa_J23114
range2330617
1
1
35
annotation2330623
1
BBa_B0012
range2330623
1
778
818
annotation2330628
1
BBa_B0011
range2330628
1
827
872
annotation2330630
1
BBa_G0002
range2330630
1
873
880
annotation2330627
1
BBa_G0002
range2330627
1
819
826
BBa_J23114
1
BBa_J23114
constitutive promoter family member
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
Later
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_J23114_sequence
1
tttatggctagctcagtcctaggtacaatgctagc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_R0051_sequence
1
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_G0002_sequence
1
tactagag
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J107034_sequence
1
tttatggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaaatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttacaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctaagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
BBa_J107033_sequence
1
atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttacaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctaagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z