LOCUS       BBa_J107035             1798 bp    DNA     linear       25-Jan-2026
DEFINITION  heat-inducible RFP
ACCESSION   BBa_J107035
VERSION     BBa_J107035.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23114
     misc_feature    36..43
                     /label=BBa_G0002
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             56..769
                     /label=BBa_J107033
     misc_feature    770..777
                     /label=BBa_G0002
     stem_loop       785..804
                     /label=T7 TE
     misc_feature    811..811
                     /label=stop
     polyA_site      805..818
                     /label=polya
     misc_feature    778..818
                     /label=BBa_B0012
     terminator      778..818
                     /label=BBa_B0012
     misc_feature    819..826
                     /label=BBa_G0002
     stem_loop       839..861
                     /label=stem_loop
     misc_feature    827..872
                     /label=BBa_B0011
     terminator      827..872
                     /label=BBa_B0011
     misc_feature    873..880
                     /label=BBa_G0002
     promoter        918..923
                     /label=-10
     promoter        895..900
                     /label=-35
     misc_feature    905..921
                     /label=OR1
     misc_feature    881..897
                     /label=OR2
     misc_feature    881..929
                     /label=BBa_R0051
     promoter        881..929
                     /label=BBa_R0051
     misc_feature    1..929
                     /label=BBa_J107034
     misc_feature    942..945
                     /label=conserved
     RBS             938..949
                     /label=BBa_B0034
     CDS             956..1630
                     /label=mrfp1
     misc_feature    1637..1661
                     /label=Help:Barcodes
     CDS             956..1636
                     /label=BBa_E1010
     stem_loop       1681..1724
                     /label=stem_loop
     misc_feature    1670..1749
                     /label=BBa_B0010
     terminator      1670..1749
                     /label=BBa_B0010
     stem_loop       1765..1784
                     /label=T7 TE
     misc_feature    1791..1791
                     /label=stop
     polyA_site      1785..1798
                     /label=polya
     misc_feature    1758..1798
                     /label=BBa_B0012
     terminator      1758..1798
                     /label=BBa_B0012
     misc_feature    938..1798
                     /label=BBa_I13507
ORIGIN
        1 tttatggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaaatgag
       61 cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat
      121 ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg
      181 gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa
      241 cgccgcattg cttacaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc
      301 cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta
      361 tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctaagc ttagaacctt
      421 taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt
      481 ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc
      541 tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat
      601 agccagactt gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt
      661 gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt
      721 tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggctgat actagagtca
      781 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa
      841 gccagattat taatccggct tttttattat tttactagag taacaccgtg cgtgttgact
      901 attttacctc tggcggtgat aatggttgct actagagaaa gaggagaaat actagatggc
      961 ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt
     1021 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca
     1081 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc
     1141 cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta
     1201 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg
     1261 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt
     1321 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg
     1381 ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa
     1441 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat
     1501 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac
     1561 ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc
     1621 caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa
     1681 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1741 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//