LOCUS       BBa_J107036             1779 bp    DNA     linear       01-Jun-2024
DEFINITION  heat-inducible TetR
ACCESSION   BBa_J107036
VERSION     BBa_J107036.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23114
     misc_feature    36..43
                     /label=BBa_G0002
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             56..769
                     /label=BBa_J107033
     misc_feature    770..777
                     /label=BBa_G0002
     stem_loop       785..804
                     /label=T7 TE
     misc_feature    811..811
                     /label=stop
     polyA_site      805..818
                     /label=polya
     misc_feature    778..818
                     /label=BBa_B0012
     terminator      778..818
                     /label=BBa_B0012
     misc_feature    819..826
                     /label=BBa_G0002
     stem_loop       839..861
                     /label=stem_loop
     misc_feature    827..872
                     /label=BBa_B0011
     terminator      827..872
                     /label=BBa_B0011
     misc_feature    873..880
                     /label=BBa_G0002
     promoter        918..923
                     /label=-10
     promoter        895..900
                     /label=-35
     misc_feature    905..921
                     /label=OR1
     misc_feature    881..897
                     /label=OR2
     misc_feature    881..929
                     /label=BBa_R0051
     promoter        881..929
                     /label=BBa_R0051
     misc_feature    1..929
                     /label=BBa_J107034
     misc_feature    944..947
                     /label=conserved
     RBS             938..951
                     /label=BBa_B0031
     CDS             961..1577
                     /label=tetR
     misc_feature    1578..1611
                     /label=SsrA
     misc_feature    1618..1642
                     /label=Help:Barcodes
     CDS             958..1617
                     /label=BBa_C0040
     stem_loop       1662..1705
                     /label=stem_loop
     misc_feature    1651..1730
                     /label=BBa_B0010
     terminator      1651..1730
                     /label=BBa_B0010
     stem_loop       1746..1765
                     /label=T7 TE
     misc_feature    1772..1772
                     /label=stop
     polyA_site      1766..1779
                     /label=polya
     misc_feature    1739..1779
                     /label=BBa_B0012
     terminator      1739..1779
                     /label=BBa_B0012
     misc_feature    938..1779
                     /label=BBa_P0140
ORIGIN
        1 tttatggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaaatgag
       61 cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat
      121 ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg
      181 gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa
      241 cgccgcattg cttacaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc
      301 cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta
      361 tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctaagc ttagaacctt
      421 taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt
      481 ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc
      541 tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat
      601 agccagactt gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt
      661 gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt
      721 tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggctgat actagagtca
      781 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa
      841 gccagattat taatccggct tttttattat tttactagag taacaccgtg cgtgttgact
      901 attttacctc tggcggtgat aatggttgct actagagtca cacaggaaac ctactagatg
      961 tccagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga
     1021 atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg
     1081 tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat
     1141 aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat
     1201 aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta
     1261 ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc
     1321 caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact
     1381 ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct
     1441 actactgata gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt
     1501 gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt
     1561 aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg ctttagtagc ttaataacac
     1621 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
     1681 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1741 acactggctc accttcgggt gggcctttct gcgtttata
//