##gff-version 3
##sequence-region BBa_J107036 1 1779
BBa_J107036	.	engineered_region	938	1779	.	+	0	ID=BBa_P0140;Name=BBa_P0140
BBa_J107036	.	terminator	1739	1779	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0140
BBa_J107036	.	polyA_site	1766	1779	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J107036	.	stem_loop	1746	1765	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J107036	.	engineered_region	1739	1779	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J107036	.	stop_codon	1772	1772	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J107036	.	terminator	1651	1730	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0140
BBa_J107036	.	stem_loop	1662	1705	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J107036	.	engineered_region	1651	1730	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J107036	.	ribosome_entry_site	938	951	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_P0140
BBa_J107036	.	conserved_region	944	947	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J107036	.	CDS	958	1617	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0140
BBa_J107036	.	CDS	961	1577	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J107036	.	tag	1578	1611	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J107036	.	engineered_tag	1618	1642	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J107036	.	engineered_region	1	929	.	+	0	ID=BBa_J107034;Name=BBa_J107034
BBa_J107036	.	promoter	1	35	.	+	0	ID=BBa_J23114;Name=BBa_J23114;Parent=BBa_J107034
BBa_J107036	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J107034
BBa_J107036	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J107036	.	sequence_feature	819	826	.	+	0	ID=BBa_G0002;Name=BBa_G0002;Parent=BBa_J107034
BBa_J107036	.	sequence_feature	770	777	.	+	0	ID=BBa_G0002;Name=BBa_G0002;Parent=BBa_J107034
BBa_J107036	.	terminator	827	872	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_J107034
BBa_J107036	.	stem_loop	839	861	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_J107036	.	engineered_region	827	872	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_J107036	.	terminator	778	818	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J107034
BBa_J107036	.	polyA_site	805	818	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J107036	.	stem_loop	785	804	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J107036	.	engineered_region	778	818	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J107036	.	stop_codon	811	811	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J107036	.	sequence_feature	36	43	.	+	0	ID=BBa_G0002;Name=BBa_G0002;Parent=BBa_J107034
BBa_J107036	.	CDS	56	769	.	+	0	ID=BBa_J107033;Name=BBa_J107033;Parent=BBa_J107034
BBa_J107036	.	promoter	881	929	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_J107034
BBa_J107036	.	promoter	918	923	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J107036	.	engineered_region	881	929	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J107036	.	promoter	895	900	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J107036	.	non_covalent_binding_site	881	897	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_J107036	.	non_covalent_binding_site	905	921	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J107036	.	sequence_feature	873	880	.	+	0	ID=BBa_G0002;Name=BBa_G0002;Parent=BBa_J107034
>BBa_J107036
tttatggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaaatgagcacaaaaaagaaaccatta
acacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccagg
aatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgctta
taacgccgcattgcttacaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgag
atgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcaggga
tgttctcacctaagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattc
tgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgtta
attctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttca
agaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagag
ttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctgatactagagtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttatta
ttttactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaa
acctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaag
gtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggc
tttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaa
gattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggta
cacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaa
tgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaa
gaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtg
cagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgc
tgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttata