LOCUS       BBa_J107037             1773 bp    DNA     linear       01-Jun-2024
DEFINITION  promoter-less NOT gate expressing RFP
ACCESSION   BBa_J107037
VERSION     BBa_J107037.1  
FEATURES             Location/Qualifiers
     misc_feature    7..10
                     /label=conserved
     RBS             1..14
                     /label=BBa_B0031
     CDS             24..640
                     /label=tetR
     misc_feature    641..674
                     /label=SsrA
     misc_feature    681..705
                     /label=Help:Barcodes
     CDS             21..680
                     /label=BBa_C0040
     stem_loop       725..768
                     /label=stem_loop
     misc_feature    714..793
                     /label=BBa_B0010
     terminator      714..793
                     /label=BBa_B0010
     stem_loop       809..828
                     /label=T7 TE
     misc_feature    835..835
                     /label=stop
     polyA_site      829..842
                     /label=polya
     misc_feature    802..842
                     /label=BBa_B0012
     terminator      802..842
                     /label=BBa_B0012
     misc_feature    1..842
                     /label=BBa_P0140
     misc_feature    851..869
                     /label=TetR 1
     misc_feature    851..904
                     /label=BBa_R0040
     promoter        870..875
                     /label=-35
     misc_feature    876..894
                     /label=TetR 2
     promoter        893..898
                     /label=-10
     promoter        851..904
                     /label=BBa_R0040
     misc_feature    917..920
                     /label=conserved
     RBS             913..924
                     /label=BBa_B0034
     CDS             931..1605
                     /label=mrfp1
     misc_feature    1612..1636
                     /label=Help:Barcodes
     CDS             931..1611
                     /label=BBa_E1010
     stem_loop       1656..1699
                     /label=stem_loop
     misc_feature    1645..1724
                     /label=BBa_B0010
     terminator      1645..1724
                     /label=BBa_B0010
     stem_loop       1740..1759
                     /label=T7 TE
     misc_feature    1766..1766
                     /label=stop
     polyA_site      1760..1773
                     /label=polya
     misc_feature    1733..1773
                     /label=BBa_B0012
     terminator      1733..1773
                     /label=BBa_B0012
     misc_feature    913..1773
                     /label=BBa_I13507
ORIGIN
        1 tcacacagga aacctactag atgtccagat tagataaaag taaagtgatt aacagcgcat
       61 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
      121 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
      181 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
      241 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
      301 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
      361 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
      421 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
      481 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
      541 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
      601 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact
      661 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagccaggca
      721 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      781 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      841 tatactagag tccctatcag tgatagagat tgacatccct atcagtgata gagatactga
      901 gcactactag agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag
      961 ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa
     1021 ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa
     1081 ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa
     1141 gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt
     1201 ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac
     1261 tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg
     1321 tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg
     1381 tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt
     1441 ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg
     1501 ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc
     1561 gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag
     1621 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1681 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1741 gctcaccttc gggtgggcct ttctgcgttt ata
//