LOCUS BBa_J107038 2843 bp DNA linear 25-Nov-2024 DEFINITION HSL-inducible device + NOT gate + RFP ACCESSION BBa_J107038 VERSION BBa_J107038.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 misc_feature 1..1062 /label=BBa_F2622 misc_feature 1077..1080 /label=conserved RBS 1071..1084 /label=BBa_B0031 CDS 1094..1710 /label=tetR misc_feature 1711..1744 /label=SsrA misc_feature 1751..1775 /label=Help:Barcodes CDS 1091..1750 /label=BBa_C0040 stem_loop 1795..1838 /label=stem_loop misc_feature 1784..1863 /label=BBa_B0010 terminator 1784..1863 /label=BBa_B0010 stem_loop 1879..1898 /label=T7 TE misc_feature 1905..1905 /label=stop polyA_site 1899..1912 /label=polya misc_feature 1872..1912 /label=BBa_B0012 terminator 1872..1912 /label=BBa_B0012 misc_feature 1071..1912 /label=BBa_P0140 misc_feature 1921..1939 /label=TetR 1 misc_feature 1921..1974 /label=BBa_R0040 promoter 1940..1945 /label=-35 misc_feature 1946..1964 /label=TetR 2 promoter 1963..1968 /label=-10 promoter 1921..1974 /label=BBa_R0040 misc_feature 1987..1990 /label=conserved RBS 1983..1994 /label=BBa_B0034 CDS 2001..2675 /label=mrfp1 misc_feature 2682..2706 /label=Help:Barcodes CDS 2001..2681 /label=BBa_E1010 stem_loop 2726..2769 /label=stem_loop misc_feature 2715..2794 /label=BBa_B0010 terminator 2715..2794 /label=BBa_B0010 stem_loop 2810..2829 /label=T7 TE misc_feature 2836..2836 /label=stop polyA_site 2830..2843 /label=polya misc_feature 2803..2843 /label=BBa_B0012 terminator 2803..2843 /label=BBa_B0012 misc_feature 1983..2843 /label=BBa_I13507 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag tcacacagga 1081 aacctactag atgtccagat tagataaaag taaagtgatt aacagcgcat tagagctgct 1141 taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga 1201 gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat 1261 tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga 1321 ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc 1381 aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt 1441 agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt 1501 ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga 1561 aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt 1621 tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt 1681 agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact acgctttagt 1741 agcttaataa cactgatagt gctagtgtag atcactacta gagccaggca tcaaataaaa 1801 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 1861 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag 1921 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 1981 agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt 2041 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg 2101 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc 2161 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta 2221 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg 2281 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc 2341 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc 2401 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag 2461 acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg 2521 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca 2581 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt 2641 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta 2701 gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 2761 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 2821 gggtgggcct ttctgcgttt ata //