LOCUS BBa_J107039 2710 bp DNA linear 01-Jun-2024 DEFINITION heat-inducible device + NOT gate + RFP ACCESSION BBa_J107039 VERSION BBa_J107039.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23114 misc_feature 36..43 /label=BBa_G0002 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 56..769 /label=BBa_J107033 misc_feature 770..777 /label=BBa_G0002 stem_loop 785..804 /label=T7 TE misc_feature 811..811 /label=stop polyA_site 805..818 /label=polya misc_feature 778..818 /label=BBa_B0012 terminator 778..818 /label=BBa_B0012 misc_feature 819..826 /label=BBa_G0002 stem_loop 839..861 /label=stem_loop misc_feature 827..872 /label=BBa_B0011 terminator 827..872 /label=BBa_B0011 misc_feature 873..880 /label=BBa_G0002 promoter 918..923 /label=-10 promoter 895..900 /label=-35 misc_feature 905..921 /label=OR1 misc_feature 881..897 /label=OR2 misc_feature 881..929 /label=BBa_R0051 promoter 881..929 /label=BBa_R0051 misc_feature 1..929 /label=BBa_J107034 misc_feature 944..947 /label=conserved RBS 938..951 /label=BBa_B0031 CDS 961..1577 /label=tetR misc_feature 1578..1611 /label=SsrA misc_feature 1618..1642 /label=Help:Barcodes CDS 958..1617 /label=BBa_C0040 stem_loop 1662..1705 /label=stem_loop misc_feature 1651..1730 /label=BBa_B0010 terminator 1651..1730 /label=BBa_B0010 stem_loop 1746..1765 /label=T7 TE misc_feature 1772..1772 /label=stop polyA_site 1766..1779 /label=polya misc_feature 1739..1779 /label=BBa_B0012 terminator 1739..1779 /label=BBa_B0012 misc_feature 938..1779 /label=BBa_P0140 misc_feature 1788..1806 /label=TetR 1 misc_feature 1788..1841 /label=BBa_R0040 promoter 1807..1812 /label=-35 misc_feature 1813..1831 /label=TetR 2 promoter 1830..1835 /label=-10 promoter 1788..1841 /label=BBa_R0040 misc_feature 1854..1857 /label=conserved RBS 1850..1861 /label=BBa_B0034 CDS 1868..2542 /label=mrfp1 misc_feature 2549..2573 /label=Help:Barcodes CDS 1868..2548 /label=BBa_E1010 stem_loop 2593..2636 /label=stem_loop misc_feature 2582..2661 /label=BBa_B0010 terminator 2582..2661 /label=BBa_B0010 stem_loop 2677..2696 /label=T7 TE misc_feature 2703..2703 /label=stop polyA_site 2697..2710 /label=polya misc_feature 2670..2710 /label=BBa_B0012 terminator 2670..2710 /label=BBa_B0012 misc_feature 1850..2710 /label=BBa_I13507 ORIGIN 1 tttatggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaaatgag 61 cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat 121 ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg 181 gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa 241 cgccgcattg cttacaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc 301 cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta 361 tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctaagc ttagaacctt 421 taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt 481 ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc 541 tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat 601 agccagactt gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt 661 gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt 721 tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggctgat actagagtca 781 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa 841 gccagattat taatccggct tttttattat tttactagag taacaccgtg cgtgttgact 901 attttacctc tggcggtgat aatggttgct actagagtca cacaggaaac ctactagatg 961 tccagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga 1021 atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg 1081 tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat 1141 aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat 1201 aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta 1261 ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc 1321 caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact 1381 ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct 1441 actactgata gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt 1501 gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt 1561 aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg ctttagtagc ttaataacac 1621 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 1681 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 1741 acactggctc accttcgggt gggcctttct gcgtttatat actagagtcc ctatcagtga 1801 tagagattga catccctatc agtgatagag atactgagca ctactagaga aagaggagaa 1861 atactagatg gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat 1921 ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta 1981 cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg 2041 ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga 2101 catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa 2161 cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt 2221 catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa 2281 aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa 2341 aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa 2401 aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa 2461 actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga 2521 aggtcgtcac tccaccggtg cttaataacg ctgatagtgc tagtgtagat cgctactaga 2581 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 2641 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 2701 tgcgtttata //