LOCUS       BBa_J107043             2677 bp    DNA     linear       22-Dec-2024
DEFINITION  GFP-RFP operon under Plux
ACCESSION   BBa_J107043
VERSION     BBa_J107043.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     RBS             1070..1082
                     /label=RBS-3\Weak
     RBS             1076..1079
                     /label=RBS
     misc_feature    1070..1082
                     /label=BBa_B0032
     RBS             1070..1082
                     /label=BBa_B0032
     CDS             1089..1808
                     /label=GFP protein
     CDS             1089..1808
                     /label=BBa_E0040
     misc_feature    1821..1824
                     /label=conserved
     RBS             1817..1828
                     /label=BBa_B0034
     CDS             1835..2509
                     /label=mrfp1
     misc_feature    2516..2540
                     /label=Help:Barcodes
     CDS             1835..2515
                     /label=BBa_E1010
     stem_loop       2560..2603
                     /label=stem_loop
     misc_feature    2549..2628
                     /label=BBa_B0010
     terminator      2549..2628
                     /label=BBa_B0010
     stem_loop       2644..2663
                     /label=T7 TE
     misc_feature    2670..2670
                     /label=stop
     polyA_site      2664..2677
                     /label=polya
     misc_feature    2637..2677
                     /label=BBa_B0012
     terminator      2637..2677
                     /label=BBa_B0012
     misc_feature    1817..2677
                     /label=BBa_I13507
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagagt cacacaggaa
     1081 agtactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     1141 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
     1201 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
     1261 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata
     1321 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
     1381 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
     1441 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
     1501 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
     1561 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
     1621 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     1681 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     1741 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     1801 aataataata ctagagaaag aggagaaata ctagatggct tcctccgaag acgttatcaa
     1861 agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat
     1921 cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac
     1981 caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc
     2041 caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga
     2101 aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca
     2161 ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt
     2221 cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg
     2281 tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga
     2341 cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct
     2401 gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac
     2461 catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt aataacgctg
     2521 atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     2581 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     2641 actggctcac cttcgggtgg gcctttctgc gtttata
//