LOCUS       BBa_J107044             2679 bp    DNA     linear       06-Dec-2025
DEFINITION  RFP-GFP operon under Plux
ACCESSION   BBa_J107044
VERSION     BBa_J107044.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     CDS             1088..1762
                     /label=mrfp1
     misc_feature    1769..1793
                     /label=Help:Barcodes
     CDS             1088..1793
                     /label=BBa_E1010
     RBS             1802..1816
                     /label=RBS-1\Strong
     RBS             1809..1813
                     /label=RBS
     misc_feature    1802..1816
                     /label=BBa_B0030
     RBS             1802..1816
                     /label=BBa_B0030
     CDS             1823..2542
                     /label=GFP protein
     CDS             1823..2542
                     /label=BBa_E0040
     stem_loop       2562..2605
                     /label=stem_loop
     misc_feature    2551..2630
                     /label=BBa_B0010
     terminator      2551..2630
                     /label=BBa_B0010
     stem_loop       2646..2665
                     /label=T7 TE
     misc_feature    2672..2672
                     /label=stop
     polyA_site      2666..2679
                     /label=polya
     misc_feature    2639..2679
                     /label=BBa_B0012
     terminator      2639..2679
                     /label=BBa_B0012
     terminator      2551..2679
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat
     1141 ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta
     1201 cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg
     1261 ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga
     1321 catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa
     1381 cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt
     1441 catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa
     1501 aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa
     1561 aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa
     1621 aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa
     1681 actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga
     1741 aggtcgtcac tccaccggtg cttaataacg ctgatagtgc tagtgtagat cgctactaga
     1801 gattaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc
     1861 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
     1921 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
     1981 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag
     2041 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
     2101 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
     2161 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
     2221 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
     2281 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
     2341 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
     2401 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
     2461 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
     2521 ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt
     2581 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     2641 acactggctc accttcgggt gggcctttct gcgtttata
//