##gff-version 3
##sequence-region BBa_J107050 1 5067
BBa_J107050	.	engineered_region	1	4140	.	+	0	ID=BBa_K173006;Name=BBa_K173006
BBa_J107050	.	engineered_region	911	4140	.	+	0	ID=BBa_K173004;Name=BBa_K173004;Parent=BBa_K173006
BBa_J107050	.	terminator	4012	4091	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K173004
BBa_J107050	.	stem_loop	4023	4066	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J107050	.	engineered_region	4012	4091	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J107050	.	terminator	4100	4140	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K173004
BBa_J107050	.	polyA_site	4127	4140	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J107050	.	stem_loop	4107	4126	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J107050	.	stop_codon	4133	4133	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J107050	.	engineered_region	4100	4140	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J107050	.	ribosome_entry_site	911	922	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K173004
BBa_J107050	.	conserved_region	915	918	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J107050	.	CDS	929	4003	.	+	0	ID=BBa_I732005;Name=BBa_I732005;Parent=BBa_K173004
BBa_J107050	.	stop_codon	4001	4003	.	+	0	ID=annotation1936872;Name=STOP;Parent=BBa_I732005
BBa_J107050	.	point_mutation	3952	3952	.	+	0	ID=annotation1936869;Name=C->T;Parent=BBa_I732005
BBa_J107050	.	CDS	929	4003	.	+	0	ID=annotation1936870;Name=lacZ;Parent=BBa_I732005
BBa_J107050	.	start_codon	929	931	.	+	0	ID=annotation1936871;Name=START;Parent=BBa_I732005
BBa_J107050	.	engineered_region	1	902	.	+	0	ID=BBa_K173005;Name=BBa_K173005;Parent=BBa_K173006
BBa_J107050	.	engineered_region	1	840	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_K173005
BBa_J107050	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_J107050	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J107050	.	terminator	712	791	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_J107050	.	stem_loop	723	766	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J107050	.	engineered_region	712	791	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J107050	.	terminator	800	840	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_J107050	.	polyA_site	827	840	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J107050	.	stem_loop	807	826	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J107050	.	stop_codon	833	833	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J107050	.	engineered_region	800	840	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J107050	.	CDS	19	678	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_J107050	.	engineered_tag	679	703	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J107050	.	CDS	22	638	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J107050	.	tag	639	672	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J107050	.	promoter	849	902	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K173005
BBa_J107050	.	promoter	891	896	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J107050	.	non_covalent_binding_site	874	892	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J107050	.	promoter	868	873	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J107050	.	engineered_region	849	902	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J107050	.	non_covalent_binding_site	849	867	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J107050	.	engineered_region	4149	5067	.	+	0	ID=BBa_K173000;Name=BBa_K173000
BBa_J107050	.	terminator	5027	5067	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K173000
BBa_J107050	.	polyA_site	5054	5067	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J107050	.	stem_loop	5034	5053	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J107050	.	stop_codon	5060	5060	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J107050	.	engineered_region	5027	5067	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J107050	.	CDS	4211	4930	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K173000
BBa_J107050	.	CDS	4211	4930	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J107050	.	terminator	4939	5018	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K173000
BBa_J107050	.	stem_loop	4950	4993	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J107050	.	engineered_region	4939	5018	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J107050	.	ribosome_entry_site	4192	4204	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K173000
BBa_J107050	.	engineered_region	4192	4204	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J107050	.	ribosome_entry_site	4198	4201	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J107050	.	ribosome_entry_site	4192	4204	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J107050	.	promoter	4149	4183	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K173000
>BBa_J107050
aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcg
gaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaa
taagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaa
agctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtac
atttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttc
actagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaa
gtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatc
accaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaag
tgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagatt
gacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaccatgattacggattc
actggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccct
ttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggc
gctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgt
cgtcccctcaaactggcagatgcacggttacgatgcgcccatctacaccaacgtgacctatcccattacggtcaatccg
ccgtttgttcccacggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggcc
agacgcgaattatttttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgctgggtcggttacggccagga
cagtcgtttgccgtctgaatttgacctgagcgcatttttacgcgccggagaaaaccgcctcgcggtgatggtgctgcgc
tggagtgacggcagttatctggaagatcaggatatgtggcggatgagcggcattttccgtgacgtctcgttgctgcata
aaccgactacacaaatcagcgatttccatgttgccactcgctttaatgatgatttcagccgcgctgtactggaggctga
agttcagatgtgcggcgagttgcgtgactacctacgggtaacagtttctttatggcagggtgaaacgcaggtcgccagc
ggcaccgcgcctttcggcggtgaaattatcgatgagcgtggtggttatgccgatcgcgtcacactacgtctgaacgtcg
aaaacccgaaactgtggagcgccgaaatcccgaatctctatcgtgcggtggttgaactgcacaccgccgacggcacgct
gattgaagcagaagcctgcgatgtcggtttccgcgaggtgcggattgaaaatggtctgctgctgctgaacggcaagccg
ttgctgattcgaggcgttaaccgtcacgagcatcatcctctgcatggtcaggtcatggatgagcagacgatggtgcagg
atatcctgctgatgaagcagaacaactttaacgccgtgcgctgttcgcattatccgaaccatccgctgtggtacacgct
gtgcgaccgctacggcctgtatgtggtggatgaagccaatattgaaacccacggcatggtgccaatgaatcgtctgacc
gatgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcgatcgtaatcacccgagtgtga
tcatctggtcgctggggaatgaatcaggccacggcgctaatcacgacgcgctgtatcgctggatcaaatctgtcgatcc
ttcccgcccggtgcagtatgaaggcggcggagccgacaccacggccaccgatattatttgcccgatgtacgcgcgcgtg
gatgaagaccagcccttcccggctgtgccgaaatggtccatcaaaaaatggctttcgctacctggagagacgcgcccgc
tgatcctttgcgaatacgcccacgcgatgggtaacagtcttggcggtttcgctaaatactggcaggcgtttcgtcagta
tccccgtttacagggcggcttcgtctgggactgggtggatcagtcgctgattaaatatgatgaaaacggcaacccgtgg
tcggcttacggcggtgattttggcgatacgccgaacgatcgccagttctgtatgaacggtctggtctttgccgaccgca
cgccgcatccagcgctgacggaagcaaaacaccagcagcagtttttccagttccgtttatccgggcaaaccatcgaagt
gaccagcgaatacctgttccgtcatagcgataacgagctcctgcactggatggtggcgctggatggtaagccgctggca
agcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcg
ccgggcaactctggctcacagtacgcgtagtgcaaccgaacgcgaccgcatggtcagaagccgggcacatcagcgcctg
gcagcagtggcgtctggcggaaaacctcagtgtgacgctccccgccgcgtcccacgccatcccgcatctgaccaccagc
gaaatggatttttgcatcgagctgggtaataagcgttggcaatttaaccgccagtcaggctttctttcacagatgtgga
ttggcgataaaaaacaactgctgacgccgctgcgcgatcagttcacccgtgcaccgctggataacgacattggcgtaag
tgaagcgacccgcattgaccctaacgcctgggtcgaacgctggaaggcggcgggccattaccaggccgaagcagcgttg
ttgcagtgcacggcagatacacttgctgatgcggtgctgattacgaccgctcacgcgtggcagcatcaggggaaaacct
tatttatcagccggaaaacctaccggattgatggtagtggtcaaatggcgattaccgttgatgttgaagtggcgagcga
tacaccgcatccggcgcggattggcctgaactgccagctggcgcaggtagcagagcgggtaaactggctcggattaggg
ccgcaagaaaactatcccgaccgccttactgccgcctgttttgaccgctgggatctgccattgtcagacatgtataccc
cgtacgtcttcccgagcgaaaacggtctgcgctgcgggacgcgcgaattgaattatggcccacaccagtggcgcggcga
cttccagttcaacatcagccgctacagtcaacagcaactgatggaaaccagccatcgccatctgctgcacgcggaagaa
ggcacatggctgaatatcgacggtttccatatggggattggtggcgacgactcctggagcccgtcagtatcggcggaat
ttcagctgagcgccggtcgctaccattaccagttggtctggtgtcaaaaataatactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctact
agagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattaga
tggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaa
tttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttg
cgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactat
atttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgag
ttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtat
acatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttca
actagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccaca
caatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggatta
cacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttata