LOCUS BBa_J107051 4183 bp DNA linear 22-Dec-2024 DEFINITION Constitutive generator of Beta-Galactosidase + J23100 ACCESSION BBa_J107051 VERSION BBa_J107051.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 22..638 /label=tetR misc_feature 639..672 /label=SsrA misc_feature 679..703 /label=Help:Barcodes CDS 19..678 /label=BBa_C0040 stem_loop 723..766 /label=stem_loop misc_feature 712..791 /label=BBa_B0010 terminator 712..791 /label=BBa_B0010 stem_loop 807..826 /label=T7 TE misc_feature 833..833 /label=stop polyA_site 827..840 /label=polya misc_feature 800..840 /label=BBa_B0012 terminator 800..840 /label=BBa_B0012 misc_feature 1..840 /label=BBa_P0440 misc_feature 849..867 /label=TetR 1 misc_feature 849..902 /label=BBa_R0040 promoter 868..873 /label=-35 misc_feature 874..892 /label=TetR 2 promoter 891..896 /label=-10 promoter 849..902 /label=BBa_R0040 misc_feature 1..902 /label=BBa_K173005 misc_feature 915..918 /label=conserved RBS 911..922 /label=BBa_B0034 misc_feature 3952..3952 /label=C->T CDS 929..4003 /label=lacZ misc_feature 929..931 /label=START misc_feature 4001..4003 /label=STOP CDS 929..4003 /label=BBa_I732005 stem_loop 4023..4066 /label=stem_loop misc_feature 4012..4091 /label=BBa_B0010 terminator 4012..4091 /label=BBa_B0010 stem_loop 4107..4126 /label=T7 TE misc_feature 4133..4133 /label=stop polyA_site 4127..4140 /label=polya misc_feature 4100..4140 /label=BBa_B0012 terminator 4100..4140 /label=BBa_B0012 misc_feature 911..4140 /label=BBa_K173004 misc_feature 1..4140 /label=BBa_K173006 promoter 4149..4183 /label=BBa_J23100 ORIGIN 1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta 61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta 121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc 181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc 241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc 301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa 361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc 421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct 481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc 541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata 601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac 661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc 721 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 781 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 841 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc 901 actactagag aaagaggaga aatactagat gaccatgatt acggattcac tggccgtcgt 961 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca 1021 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca 1081 gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc 1141 ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg 1201 gcagatgcac ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa 1261 tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga 1321 tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt 1381 tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga 1441 atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg 1501 ctggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg 1561 tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg 1621 ctttaatgat gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt 1681 gcgtgactac ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg 1741 caccgcgcct ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac 1801 actacgtctg aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg 1861 tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt 1921 cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct 1981 gattcgaggc gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca 2041 gacgatggtg caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc 2101 gcattatccg aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt 2161 ggatgaagcc aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc 2221 gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca 2281 cccgagtgtg atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc 2341 gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg 2401 agccgacacc acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca 2461 gcccttcccg gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac 2521 gcgcccgctg atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc 2581 taaatactgg caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg 2641 ggtggatcag tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg 2701 tgattttggc gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga 2761 ccgcacgccg catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg 2821 tttatccggg caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga 2881 gctcctgcac tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct 2941 ggatgtcgct ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag 3001 cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga 3061 agccgggcac atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct 3121 ccccgccgcg tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga 3181 gctgggtaat aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat 3241 tggcgataaa aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga 3301 taacgacatt ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg 3361 gaaggcggcg ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact 3421 tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt 3481 tatcagccgg aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt 3541 tgaagtggcg agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca 3601 ggtagcagag cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct 3661 tactgccgcc tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt 3721 cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca 3781 gtggcgcggc gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac 3841 cagccatcgc catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca 3901 tatggggatt ggtggcgacg actcctggag cccgtcagta tcggcggaat ttcagctgag 3961 cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taatactaga gccaggcatc 4021 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 4081 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 4141 tactagagtt gacggctagc tcagtcctag gtacagtgct agc //