LOCUS       BBa_J107053             1931 bp    DNA     linear       26-Dec-2024
DEFINITION  IPTG and 3OC6HSL inducible mRFP generator
ACCESSION   BBa_J107053
VERSION     BBa_J107053.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..83
                     /label=prefix
     misc_feature    255..255
                     /label=T
     misc_feature    573..573
                     /label=A
     CDS             82..831
                     /label=luxR
     misc_feature    82..837
                     /label=BBa_C0062
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             82..837
                     /label=BBa_C0062
     stem_loop       882..925
                     /label=stem_loop
     misc_feature    871..950
                     /label=BBa_B0010
     terminator      871..950
                     /label=BBa_B0010
     stem_loop       966..985
                     /label=T7 TE
     misc_feature    992..992
                     /label=stop
     polyA_site      986..999
                     /label=polya
     misc_feature    959..999
                     /label=BBa_B0012
     terminator      959..999
                     /label=BBa_B0012
     misc_feature    1008..1027
                     /label=LuxR/HSL
     promoter        1027..1032
                     /label=-35
     promoter        1049..1054
                     /label=-10
     misc_feature    1060..1060
                     /label=start
     misc_feature    1008..1062
                     /label=BBa_R0062
     promoter        1008..1062
                     /label=BBa_R0062
     misc_feature    1..1062
                     /label=BBa_F2622
     misc_feature    1075..1078
                     /label=conserved
     RBS             1071..1082
                     /label=BBa_B0034
     CDS             1089..1763
                     /label=mrfp1
     misc_feature    1770..1794
                     /label=Help:Barcodes
     CDS             1089..1769
                     /label=BBa_E1010
     stem_loop       1814..1857
                     /label=stem_loop
     misc_feature    1803..1882
                     /label=BBa_B0010
     terminator      1803..1882
                     /label=BBa_B0010
     stem_loop       1898..1917
                     /label=T7 TE
     misc_feature    1924..1924
                     /label=stop
     polyA_site      1918..1931
                     /label=polya
     misc_feature    1891..1931
                     /label=BBa_B0012
     terminator      1891..1931
                     /label=BBa_B0012
     misc_feature    1071..1931
                     /label=BBa_I13507
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt
      121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact
      181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa
      241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct
      301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat
      361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg
      421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc
      481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat
      541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat
      601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg
      661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact
      721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt
      781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac
      841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg
     1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga
     1081 aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta
     1141 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt
     1201 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt
     1261 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg
     1321 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga
     1381 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt
     1441 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga
     1501 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga
     1561 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta
     1621 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca
     1681 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg
     1741 aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag
     1801 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1861 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1921 ctgcgtttat a
//