LOCUS       BBa_J107054             1375 bp    DNA     linear       01-Jun-2024
DEFINITION  adhB inducible system (aTc)
ACCESSION   BBa_J107054
VERSION     BBa_J107054.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     CDS             84..1238
                     /label=BBa_K173017
     stem_loop       1258..1301
                     /label=stem_loop
     misc_feature    1247..1326
                     /label=BBa_B0010
     terminator      1247..1326
                     /label=BBa_B0010
     stem_loop       1342..1361
                     /label=T7 TE
     misc_feature    1368..1368
                     /label=stop
     polyA_site      1362..1375
                     /label=polya
     misc_feature    1335..1375
                     /label=BBa_B0012
     terminator      1335..1375
                     /label=BBa_B0012
     misc_feature    63..1375
                     /label=BBa_K173019
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatggcat caagcacctt ttatatcccg tttgttaatg
      121 aaatgggcga gggttctctg gaaaaagcga tcaaagacct gaatggtagt gggttcaaaa
      181 atgccctgat tgtgtcagac gccttcatga ataaaagtgg cgtggtgaaa caggttgctg
      241 atctgctgaa agcacagggt atcaatagcg ccgtgtatga cggtgttatg ccgaatccga
      301 ccgttacagc cgtactggag ggactgaaaa tcctgaaaga caacaacagc gacttcgtga
      361 tttctctggg tggtggttct ccacatgatt gtgccaaagc cattgccctg gtggctacaa
      421 atggaggtga agtgaaagat tatgagggga tcgacaaaag caaaaaaccg gcactgcctc
      481 tgatgagcat caataccaca gcgggtacag catctgagat gactcgtttc tgtattatca
      541 ccgacgaggt tcgccatgtg aaaatggcca tcgtggatcg tcatgttaca ccgatggtta
      601 gcgtgaatga tcctctgctg atggtgggta tgcctaaagg gctgactgcc gctacaggaa
      661 tggacgccct gactcatgcc ttcgaagcct attctagcac cgccgctact cctattaccg
      721 acgcttgtgc cctgaaagct gcctctatga ttgccaaaaa tctgaaaacc gcttgtgaca
      781 acggcaaaga catgcctgct cgtgaggcca tggcctatgc ccaatttctg gccggtatgg
      841 cgtttaataa cgcctctctg ggctatgttc atgctatggc tcaccaactg ggaggctatt
      901 ataacctgcc acacggcgtc tgtaatgctg tgctgctgcc tcatgttctg gcctataatg
      961 ccagcgtggt tgctggtcgc ctgaaagatg ttggagtggc tatgggtctg gatattgcca
     1021 atctggggga caaagagggt gctgaagcga caattcaagc cgtccgtgac ctggctgctt
     1081 ctatcggaat cccggcaaac ctgacagaac tgggcgccaa aaaagaagat gtgcctctgc
     1141 tggctgatca tgctctgaaa gacgcttgtg ctctgactaa tcctcgtcag ggagatcaga
     1201 aagaagtcga ggagctgttt ctgagtgcct tttgataata ctagagccag gcatcaaata
     1261 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1321 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//