##gff-version 3
##sequence-region BBa_J107058 1 3279
BBa_J107058	.	primer	3160	3179	.	+	0	ID=BBa_G00100;Name=BBa_G00100
BBa_J107058	.	primer_binding_site	3160	3179	.	+	0	ID=annotation1934505;Name=VF2;Parent=BBa_G00100
BBa_J107058	.	CDS	1565	3082	.	+	0	ID=BBa_J107059;Name=BBa_J107059
BBa_J107058	.	engineered_region	746	1514	.	+	0	ID=BBa_K300999;Name=BBa_K300999
BBa_J107058	.	CDS	746	1408	.	+	0	ID=annotation2081291;Name=cmR(rev);Parent=BBa_K300999
BBa_J107058	.	terminator	22	90	.	+	0	ID=BBa_B0054;Name=BBa_B0054
BBa_J107058	.	sequence_feature	25	64	.	+	0	ID=annotation1783342;Name=identical to E. coli MG1655 between tonB and yciA;Parent=BBa_B0054
BBa_J107058	.	stem_loop	32	63	.	+	0	ID=annotation1747359;Name=stem loop;Parent=BBa_B0054
BBa_J107058	.	sequence_feature	1522	1557	.	+	0	ID=BBa_J72001;Name=BBa_J72001
BBa_J107058	.	sequence_feature	91	110	.	+	0	ID=BBa_G00102;Name=BBa_G00102
BBa_J107058	.	primer_binding_site	91	110	.	+	0	ID=annotation1961229;Name=VR;Parent=BBa_G00102
BBa_J107058	.	sequence_feature	152	187	.	+	0	ID=BBa_J72001;Name=BBa_J72001
BBa_J107058	.	sequence_feature	1	21	.	+	0	ID=BBa_G00001;Name=BBa_G00001
BBa_J107058	.	sequence_feature	2	7	.	+	0	ID=annotation1934226;Name=SpeI;Parent=BBa_G00001
BBa_J107058	.	sequence_feature	16	21	.	+	0	ID=annotation1934227;Name=PstI;Parent=BBa_G00001
BBa_J107058	.	engineered_region	1	21	.	+	0	ID=annotation1821544;Name=Suffix;Parent=BBa_G00001
BBa_J107058	.	sequence_feature	9	16	.	+	0	ID=annotation1934228;Name=NotI;Parent=BBa_G00001
BBa_J107058	.	sequence_feature	738	745	.	+	0	ID=BBa_G0001;Name=BBa_G0001
BBa_J107058	.	terminator	3088	3159	.	+	0	ID=BBa_B0053;Name=BBa_B0053
BBa_J107058	.	stem_loop	3096	3130	.	+	0	ID=annotation318602;Name=stem_loop;Parent=BBa_B0053
BBa_J107058	.	sequence_feature	1515	1521	.	+	0	ID=BBa_K300995;Name=BBa_K300995
BBa_J107058	.	terminator	3180	3257	.	+	0	ID=BBa_B0055;Name=BBa_B0055
BBa_J107058	.	stem_loop	3215	3236	.	+	0	ID=annotation1783301;Name=stem loop from bacteriophage T3;Parent=BBa_B0055
BBa_J107058	.	sequence_feature	3207	3245	.	+	0	ID=annotation1783311;Name=identical to bacteriophage T3;Parent=BBa_B0055
BBa_J107058	.	sequence_feature	601	608	.	+	0	ID=BBa_G0001;Name=BBa_G0001
BBa_J107058	.	sequence_feature	3083	3087	.	+	0	ID=BBa_J107060;Name=BBa_J107060
BBa_J107058	.	terminator	111	151	.	+	0	ID=BBa_B0062;Name=BBa_B0062
BBa_J107058	.	stem_loop	124	143	.	+	0	ID=annotation318637;Name=stem_loop;Parent=BBa_B0062
BBa_J107058	.	sequence_feature	195	600	.	+	0	ID=BBa_K300998;Name=BBa_K300998
BBa_J107058	.	sequence_feature	188	194	.	+	0	ID=BBa_K300994;Name=BBa_K300994
BBa_J107058	.	terminator	609	737	.	+	0	ID=BBa_B0025;Name=BBa_B0025
BBa_J107058	.	engineered_region	609	649	.	+	0	ID=annotation369702;Name=B0012;Parent=BBa_B0025
BBa_J107058	.	engineered_region	658	737	.	+	0	ID=annotation369703;Name=B0010;Parent=BBa_B0025
BBa_J107058	.	sequence_feature	3258	3279	.	+	0	ID=BBa_G00000;Name=BBa_G00000
BBa_J107058	.	sequence_feature	3273	3278	.	+	0	ID=annotation1934224;Name=XbaI site;Parent=BBa_G00000
BBa_J107058	.	sequence_feature	3258	3263	.	+	0	ID=annotation1934223;Name=EcoRI site;Parent=BBa_G00000
BBa_J107058	.	engineered_region	3258	3279	.	+	0	ID=annotation1934225;Name=Prefix;Parent=BBa_G00000
BBa_J107058	.	sequence_feature	3264	3271	.	+	0	ID=annotation1961228;Name=NotI site;Parent=BBa_G00000
BBa_J107058	.	sequence_feature	1558	1564	.	+	0	ID=BBa_G0005;Name=BBa_G0005
>BBa_J107058
tactagtagcggccgctgcagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttgggg
ttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgcgaagt
tcctatactttctagagaataggaacttccctagtagcagttcaacctgttgatagtacgtactaagctctcatgtttc
acgtactaagctctcatgtttaacgtactaagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctc
tcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaacaataaaattaatataaatcagca
acttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaac
ggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctcaaagcaattttgagtgacacagga
acacttaacggctgacatgggaattagccatgggcccgtgcgaatcactctagtatataaacgcagaaaggcccacccg
aaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactg
agcctttcgttttatttgatgcctggctctagtattattacgccccgccctgccactcatcacagtactgttgtaattc
attaagcatgcggccgacatggaagccatcacaaacggcatgatgaacctggatcgccagcggcatcagcaccttgtcg
ccttgcgtataatatttgcccatcgtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactgg
tgaaactcacccagggattggctgagacaaaaaacatattctcaataaaccctttagggaaataggccaggttttcacc
gtaacacgccacatcttgcgaatatatgtggagaaactgccggaaatcgtcgtggtattcactccagagggacgaaaac
gtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccagatcaccagctcaccgtctttcatggcca
tacgaaactccgggtgagcgttcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctt
tacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctca
aaatgttctttacgatgccattgggatatatcaacggtggtatatcccgtgatttttttctccattttagcttccttag
ctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttac
gtgcccgatcaacctctagggcgaagttcctatactttctagagaataggaacttcgctagtattattaagctactaaa
gcgtagttttcgtcgtttgcagcctgttcgctttcatcagtatagcgttttgctttgtaagccgggtgcatcagattct
gtacggagaaaatatcgtcaagttccgcttcagtcaacagaccgcgttccagaacgacttcacgtacactcttaccggt
ttcggcacagattttacccacgatgtcaccgttgtggtgaccgatgaacgggttcaggtaagtaacgataccgatagag
ttgtaaacgtaaccttcgcacacttctttgttagcagtgatgccgttaatgcatttttccagcaggttgtagcaagcgt
tggtcagaatgtgaacggattcgaacatggcctggccaatgaccggctccataacgttcaattgcagctgacctgcttc
tgctgccatggtaacagtggtgtcgttaccgatgactttgaagcatacctggttaaccacttccggaacaaccgggttt
actttagctggcatgatggaagagcccgcttgcagttccggcaggttgatctcgttcaggccggcacgtgggcctgaag
agagcaagcgcaggtcgttacagattttggacatcttcacagccaggcgtttcagcgcgccgtgaaccataacataagc
gccgcagtcagaggtcgcttcgatcaggtcttcagccggtacgcatgggaagccagtaacttcagccagttttttcact
gccagcggagagtactctttcggcgtgttcagaccagtaccgattgctgttgcaccaaggttaacttccagcagcagtt
cagcggtacgttggatgtttttcacttcttctttcagcaggatgctgaaagcgcgaaattcctgaccgagggtcatcgg
tactgcgtcttgcagctgggtacgacccattttcaggatgtcctgaaattcgacagctttacgttcaaagccttcacgc
agttggttaatcgcatctaccagcttaatcagggaagagtaaactgcgatacggaaaccggtcgggtaggcgtcgttag
tggactgacatttgttaacatggtcgttcgggttcaggtactgatattcacctttttggtgacccatcagttccagacc
gatattggccagcacttcgttggtgttcatgtttacggaagtacctgcgccgccctggtagacgtctaccgggaactga
tccatgcattttccgttgttcaggacttcatcacatgcggcaatgatggcattcgctacacttttaggaatggtttgca
gctctttgtttgccatagctgcggcttttttaaccattaccataccgcgaacaaattcaggaatatcactgattttgtt
gttgctgatatagaagttttcaatcgctctcagagtgtgaacaccatagtaggcatcagctggaacttccctggtaccc
aacagatcttcttcgatacgaatgttgtttgacatgtgaaccttctttttcaagctgccaatgatttgctttaaacaca
tctagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttt
gccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagt
gagttgattgctacgtaagaattcgcggccgcttctagag