BBa_K300994 1 scar SpeI/AvrII mixed site (when SpeI comes from RFC10 Prefix) - rev 2010-10-01T11:00:00Z 2015-05-08T01:11:52Z - Reverse complement of <partinfo>BBa_K300996</partinfo>. false false _420_ 0 2621 9 Not in stock false See <partinfo>BBa_K300996</partinfo> false Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni BBa_K300999 1 CmR chloramphenicol resistance cassette (rev) 2010-10-01T11:00:00Z 2015-05-08T01:11:52Z <partinfo>BBa_P1004</partinfo> Reverse complement of <partinfo>BBa_P1004</partinfo> (chloramphenicol resistance cassette without transcriptional terminator). false false _420_ 0 2621 9 Not in stock false see <partinfo>BBa_P1004</partinfo> for detailed information. false Lorenzo Pasotti, Manuel Lupotto, Paolo Magni annotation2081291 1 cmR(rev) range2081291 1 1 663 BBa_B0062 1 BBa_B0062 Terminator (Reverse B0052) 2004-01-29T12:00:00Z 2015-08-31T04:07:21Z Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon. false false _1_ 0 24 7 Not in stock false false Caitlin Conboy annotation318637 1 stem_loop range318637 1 14 33 BBa_J72001 1 FRT {FRT} recombination site for flp recombinase in BBb 2008-07-14T11:00:00Z 2015-05-08T01:08:26Z Later Later false false _171_ 0 483 171 Not in stock false N/A false John Anderson BBa_J107058 1 BBa_J107058 BioBrick integrative vector targeting aspA locus 2012-08-25T11:00:00Z 2015-08-31T04:08:25Z <partinfo>BBa_K300000</partinfo> specialized to target aspA locus. It has been derived from <partinfo>BBa_K300000</partinfo> base vector by exciding the default integration guide (attP of phi80 phage) and ligating aspA (<partinfo>BBa_C0083</partinfo>) as guide. false false _587_ 0 2621 9 Not in stock false It can be propagated in strains with pir or pir-116 locus. Integration works only in host strains with an intact aspA genomic locus. false Lorenzo Pasotti component2181150 1 BBa_B0025 component2181139 1 BBa_B0054 component2181165 1 BBa_B0055 component2181145 1 BBa_K300994 component2181158 1 BBa_J107060 component2181170 1 BBa_G00000 component2181156 1 BBa_G0005 component2181144 1 BBa_J72001 component2181162 1 BBa_G00100 component2181136 1 BBa_G00001 component2181157 1 BBa_J107059 component2181153 1 BBa_K300999 component2181151 1 BBa_G0001 component2181141 1 BBa_G00102 component2181155 1 BBa_J72001 component2181160 1 BBa_B0053 component2181147 1 BBa_G0001 component2181143 1 BBa_B0062 component2181146 1 BBa_K300998 component2181154 1 BBa_K300995 annotation2181170 1 BBa_G00000 range2181170 1 3258 3279 annotation2181160 1 BBa_B0053 range2181160 1 3088 3159 annotation2181146 1 BBa_K300998 range2181146 1 195 600 annotation2181136 1 BBa_G00001 range2181136 1 1 21 annotation2181150 1 BBa_B0025 range2181150 1 609 737 annotation2181158 1 BBa_J107060 range2181158 1 3083 3087 annotation2181151 1 BBa_G0001 range2181151 1 738 745 annotation2181162 1 BBa_G00100 range2181162 1 3160 3179 annotation2181145 1 BBa_K300994 range2181145 1 188 194 annotation2181141 1 BBa_G00102 range2181141 1 91 110 annotation2181153 1 BBa_K300999 range2181153 1 746 1514 annotation2181144 1 BBa_J72001 range2181144 1 152 187 annotation2181139 1 BBa_B0054 range2181139 1 22 90 annotation2181156 1 BBa_G0005 range2181156 1 1558 1564 annotation2181155 1 BBa_J72001 range2181155 1 1522 1557 annotation2181143 1 BBa_B0062 range2181143 1 111 151 annotation2181147 1 BBa_G0001 range2181147 1 601 608 annotation2181157 1 BBa_J107059 range2181157 1 1565 3082 annotation2181154 1 BBa_K300995 range2181154 1 1515 1521 annotation2181165 1 BBa_B0055 range2181165 1 3180 3257 BBa_B0054 1 BBa_B0054 Transcriptional terminator 2005-11-16T12:00:00Z 2015-08-31T04:07:20Z Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051. false false _41_6_ 0 126 44 Not in stock false false Reshma Shetty annotation1747359 1 stem loop range1747359 1 11 42 annotation1783342 1 identical to E. coli MG1655 between tonB and yciA range1783342 1 4 43 BBa_B0053 1 BBa_B0053 Terminator (His) 2004-01-29T12:00:00Z 2015-08-31T04:07:20Z Terminator from the E.coli His operon false true _1_ 0 24 7 Not in stock false false Caitlin Conboy annotation318602 1 stem_loop range318602 1 9 43 BBa_G00102 1 VR Reverse BioBrick primer annealing site (VR binding site) 2006-02-03T12:00:00Z 2015-08-31T04:07:28Z This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101. false false _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1961229 1 VR range1961229 1 1 20 BBa_B0055 1 BBa_B0055 -- No description -- 2005-11-16T12:00:00Z 2015-08-31T04:07:20Z Terminator to be placed upstream of multiple cloning site in new pSB series plasmids. false false _41_6_ 0 126 44 Not in stock false false Reshma Shetty annotation1783311 1 identical to bacteriophage T3 range1783311 1 28 66 annotation1783301 1 stem loop from bacteriophage T3 range1783301 1 36 57 BBa_G0001 1 scar XbaI/SpeI mixed site, reverse 2007-02-26T12:00:00Z 2015-08-31T04:07:27Z XbaI and SpeI XbaI/SpeI mixed site, reverse false false _41_ 0 126 70 Not in stock false None. false Reshma Shetty BBa_B0025 1 BBa_B0025 double terminator (B0015), reversed 2003-12-02T12:00:00Z 2015-08-31T04:07:20Z -- No description -- false true _1_ 0 24 7 In stock false true Caitlin Conboy annotation369702 1 B0012 range369702 1 1 41 annotation369703 1 B0010 range369703 1 50 129 BBa_G00000 1 Prefix BioBrick cloning site prefix 2006-03-13T12:00:00Z 2015-08-31T04:07:27Z This part is the standard BioBricks prefix. false true _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1934224 1 XbaI site range1934224 1 16 21 annotation1961228 1 NotI site range1961228 1 7 14 annotation1934225 1 Prefix range1934225 1 1 22 annotation1934223 1 EcoRI site range1934223 1 1 6 BBa_K300998 1 R6K ori [R6K] Origin of replication (rev) 2010-10-01T11:00:00Z 2015-05-08T01:11:52Z See <partinfo>BBa_J61001</partinfo> for detailed information. Reverse complement of <partinfo>BBa_J61001</partinfo> (R6K conditional replication origin). false false _420_ 0 2621 9 Not in stock false <partinfo>BBa_J61001</partinfo> false Lorenzo Pasotti, Manuel Lupotto, Paolo Magni BBa_J107059 1 aspA(rev) aspA coding sequence (reverse) 2012-08-25T11:00:00Z 2015-08-31T04:08:25Z Same as <partinfo>BBa_C0083</partinfo>. This is <partinfo>BBa_C0083</partinfo> reversed. false false _587_ 0 2621 9 Not in stock false BioBrick format. false Lorenzo Pasotti BBa_J107060 1 scar XbaI/NheI mixed site without the initial CT nucleotides 2012-08-25T11:00:00Z 2015-08-31T04:08:25Z Registry. It is the same as <partinfo>BBa_G0006</partinfo>, but it does not have the initial CT nucleotides. It is useful to submit XbaI/NheI fusions where the BioBrick with XbaI is a coding sequence (so XbaI is TCTAG instead of TCTAGAG). false false _587_ 0 2621 9 Not in stock false Digested XbaI ligated with digested NheI. false Lorenzo Pasotti BBa_G00100 1 VF2 Forward primer for sequencing/amplifying BioBrick parts (VF2) 2004-05-24T11:00:00Z 2015-08-31T04:07:28Z Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors. false false _11_1_ 0 60 7 Not in stock false false Tom Knight annotation1934505 1 VF2 range1934505 1 1 20 BBa_K300995 1 scar AvrII/XbaI mixed site (when XbaI comes from RFC10 Prefix) - rev 2010-10-01T11:00:00Z 2015-05-08T01:11:52Z - Reverse complement of <partinfo>BBa_K300997</partinfo> false false _420_ 0 2621 9 Not in stock false See <partinfo>BBa_K300997</partinfo> false Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni BBa_G00001 1 Suffix BioBrick cloning site suffix 2006-03-13T12:00:00Z 2015-08-31T04:07:27Z This part is the standard BioBricks suffix. false true _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1821544 1 Suffix range1821544 1 1 21 annotation1934226 1 SpeI range1934226 1 2 7 annotation1934228 1 NotI range1934228 1 9 16 annotation1934227 1 PstI range1934227 1 16 21 BBa_G0005 1 scar NheI/SpeI mixed site 2007-02-26T12:00:00Z 2015-08-31T04:07:27Z NheI and SpeI NheI/SpeI mixed site false false _41_ 0 126 70 Not in stock false None. false Reshma Shetty BBa_G0005_sequence 1 gctagta BBa_K300995_sequence 1 ctctagg BBa_J72001_sequence 1 gcgaagttcctatactttctagagaataggaacttc BBa_K300998_sequence 1 gcagttcaacctgttgatagtacgtactaagctctcatgtttcacgtactaagctctcatgtttaacgtactaagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaacaataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctcaaagcaattttgagtgacacaggaacacttaacggctgacatgggaattagccatgggcccgtgcgaatca BBa_B0062_sequence 1 cagataaaaaaaatccttagctttcgctaaggatgatttct BBa_J107059_sequence 1 ttattaagctactaaagcgtagttttcgtcgtttgcagcctgttcgctttcatcagtatagcgttttgctttgtaagccgggtgcatcagattctgtacggagaaaatatcgtcaagttccgcttcagtcaacagaccgcgttccagaacgacttcacgtacactcttaccggtttcggcacagattttacccacgatgtcaccgttgtggtgaccgatgaacgggttcaggtaagtaacgataccgatagagttgtaaacgtaaccttcgcacacttctttgttagcagtgatgccgttaatgcatttttccagcaggttgtagcaagcgttggtcagaatgtgaacggattcgaacatggcctggccaatgaccggctccataacgttcaattgcagctgacctgcttctgctgccatggtaacagtggtgtcgttaccgatgactttgaagcatacctggttaaccacttccggaacaaccgggtttactttagctggcatgatggaagagcccgcttgcagttccggcaggttgatctcgttcaggccggcacgtgggcctgaagagagcaagcgcaggtcgttacagattttggacatcttcacagccaggcgtttcagcgcgccgtgaaccataacataagcgccgcagtcagaggtcgcttcgatcaggtcttcagccggtacgcatgggaagccagtaacttcagccagttttttcactgccagcggagagtactctttcggcgtgttcagaccagtaccgattgctgttgcaccaaggttaacttccagcagcagttcagcggtacgttggatgtttttcacttcttctttcagcaggatgctgaaagcgcgaaattcctgaccgagggtcatcggtactgcgtcttgcagctgggtacgacccattttcaggatgtcctgaaattcgacagctttacgttcaaagccttcacgcagttggttaatcgcatctaccagcttaatcagggaagagtaaactgcgatacggaaaccggtcgggtaggcgtcgttagtggactgacatttgttaacatggtcgttcgggttcaggtactgatattcacctttttggtgacccatcagttccagaccgatattggccagcacttcgttggtgttcatgtttacggaagtacctgcgccgccctggtagacgtctaccgggaactgatccatgcattttccgttgttcaggacttcatcacatgcggcaatgatggcattcgctacacttttaggaatggtttgcagctctttgtttgccatagctgcggcttttttaaccattaccataccgcgaacaaattcaggaatatcactgattttgttgttgctgatatagaagttttcaatcgctctcagagtgtgaacaccatagtaggcatcagctggaacttccctggtacccaacagatcttcttcgatacgaatgttgtttgacatgtgaaccttctttttcaagctgccaatgatttgctttaaacacat BBa_B0025_sequence 1 tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctgg BBa_B0055_sequence 1 aaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaa BBa_J107058_sequence 1 tactagtagcggccgctgcagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgcgaagttcctatactttctagagaataggaacttccctagtagcagttcaacctgttgatagtacgtactaagctctcatgtttcacgtactaagctctcatgtttaacgtactaagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaacaataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctcaaagcaattttgagtgacacaggaacacttaacggctgacatgggaattagccatgggcccgtgcgaatcactctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtattattacgccccgccctgccactcatcacagtactgttgtaattcattaagcatgcggccgacatggaagccatcacaaacggcatgatgaacctggatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatcgtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacaaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtggagaaactgccggaaatcgtcgtggtattcactccagagggacgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccagatcaccagctcaccgtctttcatggccatacgaaactccgggtgagcgttcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatcccgtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaacctctagggcgaagttcctatactttctagagaataggaacttcgctagtattattaagctactaaagcgtagttttcgtcgtttgcagcctgttcgctttcatcagtatagcgttttgctttgtaagccgggtgcatcagattctgtacggagaaaatatcgtcaagttccgcttcagtcaacagaccgcgttccagaacgacttcacgtacactcttaccggtttcggcacagattttacccacgatgtcaccgttgtggtgaccgatgaacgggttcaggtaagtaacgataccgatagagttgtaaacgtaaccttcgcacacttctttgttagcagtgatgccgttaatgcatttttccagcaggttgtagcaagcgttggtcagaatgtgaacggattcgaacatggcctggccaatgaccggctccataacgttcaattgcagctgacctgcttctgctgccatggtaacagtggtgtcgttaccgatgactttgaagcatacctggttaaccacttccggaacaaccgggtttactttagctggcatgatggaagagcccgcttgcagttccggcaggttgatctcgttcaggccggcacgtgggcctgaagagagcaagcgcaggtcgttacagattttggacatcttcacagccaggcgtttcagcgcgccgtgaaccataacataagcgccgcagtcagaggtcgcttcgatcaggtcttcagccggtacgcatgggaagccagtaacttcagccagttttttcactgccagcggagagtactctttcggcgtgttcagaccagtaccgattgctgttgcaccaaggttaacttccagcagcagttcagcggtacgttggatgtttttcacttcttctttcagcaggatgctgaaagcgcgaaattcctgaccgagggtcatcggtactgcgtcttgcagctgggtacgacccattttcaggatgtcctgaaattcgacagctttacgttcaaagccttcacgcagttggttaatcgcatctaccagcttaatcagggaagagtaaactgcgatacggaaaccggtcgggtaggcgtcgttagtggactgacatttgttaacatggtcgttcgggttcaggtactgatattcacctttttggtgacccatcagttccagaccgatattggccagcacttcgttggtgttcatgtttacggaagtacctgcgccgccctggtagacgtctaccgggaactgatccatgcattttccgttgttcaggacttcatcacatgcggcaatgatggcattcgctacacttttaggaatggtttgcagctctttgtttgccatagctgcggcttttttaaccattaccataccgcgaacaaattcaggaatatcactgattttgttgttgctgatatagaagttttcaatcgctctcagagtgtgaacaccatagtaggcatcagctggaacttccctggtacccaacagatcttcttcgatacgaatgttgtttgacatgtgaaccttctttttcaagctgccaatgatttgctttaaacacatctagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaagaattcgcggccgcttctagag BBa_J107060_sequence 1 ctagc BBa_B0054_sequence 1 attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg BBa_G00000_sequence 1 gaattcgcggccgcttctagag BBa_G00001_sequence 1 tactagtagcggccgctgcag BBa_K300999_sequence 1 ttattacgccccgccctgccactcatcacagtactgttgtaattcattaagcatgcggccgacatggaagccatcacaaacggcatgatgaacctggatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatcgtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacaaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtggagaaactgccggaaatcgtcgtggtattcactccagagggacgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccagatcaccagctcaccgtctttcatggccatacgaaactccgggtgagcgttcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatcccgtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaac BBa_B0053_sequence 1 tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt BBa_G00100_sequence 1 tgccacctgacgtctaagaa BBa_G0001_sequence 1 ctctagta BBa_K300994_sequence 1 cctagta BBa_G00102_sequence 1 gctcactcaaaggcggtaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z