BBa_J107058
type: ComponentDefinition
persistent identity: BBa J107058
display id: BBa_J107058
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick integrative vector targeting aspA locus
created: 2012-08-25T11:00:00Z
modified: 2015-08-31T04:08:25Z
mutable provenance: <partinfo>BBa_K300000</partinfo> specialized to target aspA locus.
owned by: james
owned by: myers
mutable description: It has been derived from <partinfo>BBa_K300000</partinfo> base vector by exciding the default integration guide (attP of phi80 phage) and ligating aspA (<partinfo>BBa_C0083</partinfo>) as guide.
discontinued: false
dominant: false
experience: experience/Works
group u list: _587_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It can be propagated in strains with pir or pir-116 locus. Integration works only in host strains with an intact aspA genomic locus.
star: false
creator: Lorenzo Pasotti
type: DnaRegion
role: partType/Plasmid Backbone
role: SO:0000755
component: BBa_B0025
component: BBa_G0001
component: BBa_K300999
component: BBa_J72001
component: BBa_K300995
component: BBa_J107059
component: BBa_G0005
component: BBa_B0053
component: BBa_J107060
component: BBa_B0055
component: BBa_G00001
component: BBa_G00100
component: BBa_G00102
component: BBa_G00000
component: BBa_B0054
component: BBa_B0062
component: BBa_K300998
component: BBa_K300994
sequence annotation: BBa_G00001
sequence annotation: BBa_B0054
sequence annotation: BBa_G00102
sequence annotation: BBa_B0062
sequence annotation: BBa_J72001
sequence annotation: BBa_K300994
sequence annotation: BBa_K300998
sequence annotation: BBa_G0001
sequence annotation: BBa_B0025
sequence annotation: BBa_K300999
sequence annotation: BBa_K300995
sequence annotation: BBa_G0005
sequence annotation: BBa_J107059
sequence annotation: BBa_J107060
sequence annotation: BBa_B0053
sequence annotation: BBa_G00100
sequence annotation: BBa_B0055
sequence annotation: BBa_G00000
sequence: https://synbiohub.org/public/igem/BBa_J107058_sequence/1

BBa_B0025
type: Component
persistent identity: component2181150
display id: component2181150
version: 1
top level: BBa_J107058
definition: BBa_B0025
access: public

BBa_G0001
type: Component
persistent identity: component2181147
display id: component2181147
version: 1
top level: BBa_J107058
definition: scar
access: public

BBa_K300999
type: Component
persistent identity: component2181153
display id: component2181153
version: 1
top level: BBa_J107058
definition: CmR
access: public

BBa_G0001
type: Component
persistent identity: component2181151
display id: component2181151
version: 1
top level: BBa_J107058
definition: scar
access: public

BBa_J72001
type: Component
persistent identity: component2181155
display id: component2181155
version: 1
top level: BBa_J107058
definition: FRT
access: public

BBa_K300995
type: Component
persistent identity: component2181154
display id: component2181154
version: 1
top level: BBa_J107058
definition: scar
access: public

BBa_J107059
type: Component
persistent identity: component2181157
display id: component2181157
version: 1
top level: BBa_J107058
definition: aspA(rev)
access: public

BBa_G0005
type: Component
persistent identity: component2181156
display id: component2181156
version: 1
top level: BBa_J107058
definition: scar
access: public

BBa_B0053
type: Component
persistent identity: component2181160
display id: component2181160
version: 1
top level: BBa_J107058
definition: BBa_B0053
access: public

BBa_J107060
type: Component
persistent identity: component2181158
display id: component2181158
version: 1
top level: BBa_J107058
definition: scar
access: public

BBa_B0055
type: Component
persistent identity: component2181165
display id: component2181165
version: 1
top level: BBa_J107058
definition: BBa_B0055
access: public

BBa_G00001
type: Component
persistent identity: component2181136
display id: component2181136
version: 1
top level: BBa_J107058
definition: Suffix
access: public

BBa_G00100
type: Component
persistent identity: component2181162
display id: component2181162
version: 1
top level: BBa_J107058
definition: VF2
access: public

BBa_G00102
type: Component
persistent identity: component2181141
display id: component2181141
version: 1
top level: BBa_J107058
definition: VR
access: public

BBa_G00000
type: Component
persistent identity: component2181170
display id: component2181170
version: 1
top level: BBa_J107058
definition: Prefix
access: public

BBa_B0054
type: Component
persistent identity: component2181139
display id: component2181139
version: 1
top level: BBa_J107058
definition: BBa_B0054
access: public

BBa_J72001
type: Component
persistent identity: component2181144
display id: component2181144
version: 1
top level: BBa_J107058
definition: FRT
access: public

BBa_B0062
type: Component
persistent identity: component2181143
display id: component2181143
version: 1
top level: BBa_J107058
definition: BBa_B0062
access: public

BBa_K300998
type: Component
persistent identity: component2181146
display id: component2181146
version: 1
top level: BBa_J107058
definition: R6K ori
access: public

BBa_K300994
type: Component
persistent identity: component2181145
display id: component2181145
version: 1
top level: BBa_J107058
definition: scar
access: public

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2181136
display id: annotation2181136
version: 1
top level: BBa_J107058
location: https://synbiohub.org/public/igem/BBa_J107058/annotation2181136/range2181136/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_J107058/annotation2181136/range2181136/1
type: Range
persistent identity: range2181136
display id: range2181136
version: 1
top level: BBa_J107058
direction: direction/reverse
start: 1
end: 21
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2181139
display id: annotation2181139
version: 1
top level: BBa_J107058
location: https://synbiohub.org/public/igem/BBa_J107058/annotation2181139/range2181139/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_J107058/annotation2181139/range2181139/1
type: Range
persistent identity: range2181139
display id: range2181139
version: 1
top level: BBa_J107058
direction: direction/reverse
start: 22
end: 90
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2181141
display id: annotation2181141
version: 1
top level: BBa_J107058
location: https://synbiohub.org/public/igem/BBa_J107058/annotation2181141/range2181141/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_J107058/annotation2181141/range2181141/1
type: Range
persistent identity: range2181141
display id: range2181141
version: 1
top level: BBa_J107058
direction: direction/reverse
start: 91
end: 110
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2181143
display id: annotation2181143
version: 1
top level: BBa_J107058
location: https://synbiohub.org/public/igem/BBa_J107058/annotation2181143/range2181143/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_J107058/annotation2181143/range2181143/1
type: Range
persistent identity: range2181143
display id: range2181143
version: 1
top level: BBa_J107058
direction: direction/reverse
start: 111
end: 151
orientation: inline

BBa_J72001
type: SequenceAnnotation
persistent identity: annotation2181144
display id: annotation2181144
version: 1
top level: BBa_J107058
location: https://synbiohub.org/public/igem/BBa_J107058/annotation2181144/range2181144/1
component: BBa_J72001

https://synbiohub.org/public/igem/BBa_J107058/annotation2181144/range2181144/1
type: Range
persistent identity: range2181144
display id: range2181144
version: 1
top level: BBa_J107058
direction: direction/reverse
start: 152
end: 187
orientation: inline

BBa_K300994
type: SequenceAnnotation
persistent identity: annotation2181145
display id: annotation2181145
version: 1
top level: BBa_J107058
location: https://synbiohub.org/public/igem/BBa_J107058/annotation2181145/range2181145/1
component: BBa_K300994

https://synbiohub.org/public/igem/BBa_J107058/annotation2181145/range2181145/1
type: Range
persistent identity: range2181145
display id: range2181145
version: 1
top level: BBa_J107058
direction: direction/reverse
start: 188
end: 194
orientation: inline

BBa_K300998
type: SequenceAnnotation
persistent identity: annotation2181146
display id: annotation2181146
version: 1
top level: BBa_J107058
location: https://synbiohub.org/public/igem/BBa_J107058/annotation2181146/range2181146/1
component: BBa_K300998

https://synbiohub.org/public/igem/BBa_J107058/annotation2181146/range2181146/1
type: Range
persistent identity: range2181146
display id: range2181146
version: 1
top level: BBa_J107058
direction: direction/reverse
start: 195
end: 600
orientation: inline

BBa_G0001
type: SequenceAnnotation
persistent identity: annotation2181147
display id: annotation2181147
version: 1
top level: BBa_J107058
location: https://synbiohub.org/public/igem/BBa_J107058/annotation2181147/range2181147/1
component: BBa_G0001

https://synbiohub.org/public/igem/BBa_J107058/annotation2181147/range2181147/1
type: Range
persistent identity: range2181147
display id: range2181147
version: 1
top level: BBa_J107058
direction: direction/reverse
start: 601
end: 608
orientation: inline

BBa_B0025
type: SequenceAnnotation
persistent identity: annotation2181150
display id: annotation2181150
version: 1
top level: BBa_J107058
location: https://synbiohub.org/public/igem/BBa_J107058/annotation2181150/range2181150/1
component: BBa_B0025

https://synbiohub.org/public/igem/BBa_J107058/annotation2181150/range2181150/1
type: Range
persistent identity: range2181150
display id: range2181150
version: 1
top level: BBa_J107058
direction: direction/reverse
start: 609
end: 737
orientation: inline

BBa_G0001
type: SequenceAnnotation
persistent identity: annotation2181151
display id: annotation2181151
version: 1
top level: BBa_J107058
location: https://synbiohub.org/public/igem/BBa_J107058/annotation2181151/range2181151/1
component: BBa_G0001

https://synbiohub.org/public/igem/BBa_J107058/annotation2181151/range2181151/1
type: Range
persistent identity: range2181151
display id: range2181151
version: 1
top level: BBa_J107058
direction: direction/reverse
start: 738
end: 745
orientation: inline

BBa_K300999
type: SequenceAnnotation
persistent identity: annotation2181153
display id: annotation2181153
version: 1
top level: BBa_J107058
location: https://synbiohub.org/public/igem/BBa_J107058/annotation2181153/range2181153/1
component: BBa_K300999

https://synbiohub.org/public/igem/BBa_J107058/annotation2181153/range2181153/1
type: Range
persistent identity: range2181153
display id: range2181153
version: 1
top level: BBa_J107058
direction: direction/reverse
start: 746
end: 1514
orientation: inline

BBa_K300995
type: SequenceAnnotation
persistent identity: annotation2181154
display id: annotation2181154
version: 1
top level: BBa_J107058
location: https://synbiohub.org/public/igem/BBa_J107058/annotation2181154/range2181154/1
component: BBa_K300995

https://synbiohub.org/public/igem/BBa_J107058/annotation2181154/range2181154/1
type: Range
persistent identity: range2181154
display id: range2181154
version: 1
top level: BBa_J107058
direction: direction/reverse
start: 1515
end: 1521
orientation: inline

BBa_J72001
type: SequenceAnnotation
persistent identity: annotation2181155
display id: annotation2181155
version: 1
top level: BBa_J107058
location: https://synbiohub.org/public/igem/BBa_J107058/annotation2181155/range2181155/1
component: BBa_J72001

https://synbiohub.org/public/igem/BBa_J107058/annotation2181155/range2181155/1
type: Range
persistent identity: range2181155
display id: range2181155
version: 1
top level: BBa_J107058
direction: direction/reverse
start: 1522
end: 1557
orientation: inline

BBa_G0005
type: SequenceAnnotation
persistent identity: annotation2181156
display id: annotation2181156
version: 1
top level: BBa_J107058
location: https://synbiohub.org/public/igem/BBa_J107058/annotation2181156/range2181156/1
component: BBa_G0005

https://synbiohub.org/public/igem/BBa_J107058/annotation2181156/range2181156/1
type: Range
persistent identity: range2181156
display id: range2181156
version: 1
top level: BBa_J107058
direction: direction/reverse
start: 1558
end: 1564
orientation: inline

BBa_J107059
type: SequenceAnnotation
persistent identity: annotation2181157
display id: annotation2181157
version: 1
top level: BBa_J107058
location: https://synbiohub.org/public/igem/BBa_J107058/annotation2181157/range2181157/1
component: BBa_J107059

https://synbiohub.org/public/igem/BBa_J107058/annotation2181157/range2181157/1
type: Range
persistent identity: range2181157
display id: range2181157
version: 1
top level: BBa_J107058
direction: direction/reverse
start: 1565
end: 3082
orientation: inline

BBa_J107060
type: SequenceAnnotation
persistent identity: annotation2181158
display id: annotation2181158
version: 1
top level: BBa_J107058
location: https://synbiohub.org/public/igem/BBa_J107058/annotation2181158/range2181158/1
component: BBa_J107060

https://synbiohub.org/public/igem/BBa_J107058/annotation2181158/range2181158/1
type: Range
persistent identity: range2181158
display id: range2181158
version: 1
top level: BBa_J107058
direction: direction/reverse
start: 3083
end: 3087
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2181160
display id: annotation2181160
version: 1
top level: BBa_J107058
location: https://synbiohub.org/public/igem/BBa_J107058/annotation2181160/range2181160/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_J107058/annotation2181160/range2181160/1
type: Range
persistent identity: range2181160
display id: range2181160
version: 1
top level: BBa_J107058
direction: direction/reverse
start: 3088
end: 3159
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2181162
display id: annotation2181162
version: 1
top level: BBa_J107058
location: https://synbiohub.org/public/igem/BBa_J107058/annotation2181162/range2181162/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_J107058/annotation2181162/range2181162/1
type: Range
persistent identity: range2181162
display id: range2181162
version: 1
top level: BBa_J107058
direction: direction/reverse
start: 3160
end: 3179
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2181165
display id: annotation2181165
version: 1
top level: BBa_J107058
location: https://synbiohub.org/public/igem/BBa_J107058/annotation2181165/range2181165/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_J107058/annotation2181165/range2181165/1
type: Range
persistent identity: range2181165
display id: range2181165
version: 1
top level: BBa_J107058
direction: direction/reverse
start: 3180
end: 3257
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2181170
display id: annotation2181170
version: 1
top level: BBa_J107058
location: https://synbiohub.org/public/igem/BBa_J107058/annotation2181170/range2181170/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_J107058/annotation2181170/range2181170/1
type: Range
persistent identity: range2181170
display id: range2181170
version: 1
top level: BBa_J107058
direction: direction/reverse
start: 3258
end: 3279
orientation: inline

BBa_B0025
type: ComponentDefinition
persistent identity: BBa B0025
display id: BBa_B0025
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0015), reversed
created: 2003-12-02T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B0012
sequence annotation: B0010
sequence: https://synbiohub.org/public/igem/BBa_B0025_sequence/1

B0012
type: SequenceAnnotation
persistent identity: annotation369702
display id: annotation369702
version: 1
top level: BBa_B0025
location: https://synbiohub.org/public/igem/BBa_B0025/annotation369702/range369702/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0025/annotation369702/range369702/1
type: Range
persistent identity: range369702
display id: range369702
version: 1
top level: BBa_B0025
direction: direction/reverse
start: 1
end: 41
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation369703
display id: annotation369703
version: 1
top level: BBa_B0025
location: https://synbiohub.org/public/igem/BBa_B0025/annotation369703/range369703/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0025/annotation369703/range369703/1
type: Range
persistent identity: range369703
display id: range369703
version: 1
top level: BBa_B0025
direction: direction/reverse
start: 50
end: 129
orientation: inline

BBa_B0053
type: ComponentDefinition
persistent identity: BBa B0053
display id: BBa_B0053
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (His)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator from the E.coli His operon
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0053_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318602
display id: annotation318602
version: 1
top level: BBa_B0053
location: https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
type: Range
persistent identity: range318602
display id: range318602
version: 1
top level: BBa_B0053
direction: direction/forward
start: 9
end: 43
orientation: inline

BBa_B0054
type: ComponentDefinition
persistent identity: BBa B0054
display id: BBa_B0054
version: 1
was generated by: iGEM to SBOL conversion
description: Transcriptional terminator
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to E. coli MG1655 between tonB and yciA
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B0054_sequence/1

identical to E. coli MG1655 between tonB and yciA
type: SequenceAnnotation
persistent identity: annotation1783342
display id: annotation1783342
version: 1
top level: BBa_B0054
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
type: Range
persistent identity: range1783342
display id: range1783342
version: 1
top level: BBa_B0054
direction: direction/forward
start: 4
end: 43
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1747359
display id: annotation1747359
version: 1
top level: BBa_B0054
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
type: Range
persistent identity: range1747359
display id: range1747359
version: 1
top level: BBa_B0054
direction: direction/forward
start: 11
end: 42
orientation: inline

BBa_B0055
type: ComponentDefinition
persistent identity: BBa B0055
display id: BBa_B0055
version: 1
was generated by: iGEM to SBOL conversion
description: -- No description --
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to bacteriophage T3
sequence annotation: stem loop from bacteriophage T3
sequence: https://synbiohub.org/public/igem/BBa_B0055_sequence/1

identical to bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783311
display id: annotation1783311
version: 1
top level: BBa_B0055
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1
type: Range
persistent identity: range1783311
display id: range1783311
version: 1
top level: BBa_B0055
direction: direction/reverse
start: 28
end: 66
orientation: inline

stem loop from bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783301
display id: annotation1783301
version: 1
top level: BBa_B0055
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1
type: Range
persistent identity: range1783301
display id: range1783301
version: 1
top level: BBa_B0055
direction: direction/reverse
start: 36
end: 57
orientation: inline

Prefix
type: ComponentDefinition
persistent identity: BBa G00000
display id: BBa_G00000
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick cloning site prefix
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: This part is the standard BioBricks prefix.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Prefix
sequence annotation: EcoRI site
sequence annotation: NotI site
sequence annotation: XbaI site
sequence: https://synbiohub.org/public/igem/BBa_G00000_sequence/1

Prefix
type: SequenceAnnotation
persistent identity: annotation1934225
display id: annotation1934225
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934225/range1934225/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_G00000/annotation1934225/range1934225/1
type: Range
persistent identity: range1934225
display id: range1934225
version: 1
top level: Prefix
direction: direction/forward
start: 1
end: 22
orientation: inline

EcoRI site
type: SequenceAnnotation
persistent identity: annotation1934223
display id: annotation1934223
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934223/range1934223/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1934223/range1934223/1
type: Range
persistent identity: range1934223
display id: range1934223
version: 1
top level: Prefix
direction: direction/forward
start: 1
end: 6
orientation: inline

NotI site
type: SequenceAnnotation
persistent identity: annotation1961228
display id: annotation1961228
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1961228/range1961228/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1961228/range1961228/1
type: Range
persistent identity: range1961228
display id: range1961228
version: 1
top level: Prefix
direction: direction/forward
start: 7
end: 14
orientation: inline

XbaI site
type: SequenceAnnotation
persistent identity: annotation1934224
display id: annotation1934224
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934224/range1934224/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1934224/range1934224/1
type: Range
persistent identity: range1934224
display id: range1934224
version: 1
top level: Prefix
direction: direction/forward
start: 16
end: 21
orientation: inline

Suffix
type: ComponentDefinition
persistent identity: BBa G00001
display id: BBa_G00001
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick cloning site suffix
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: This part is the standard BioBricks suffix.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Suffix
sequence annotation: SpeI
sequence annotation: NotI
sequence annotation: PstI
sequence: https://synbiohub.org/public/igem/BBa_G00001_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation1821544
display id: annotation1821544
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1821544/range1821544/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_G00001/annotation1821544/range1821544/1
type: Range
persistent identity: range1821544
display id: range1821544
version: 1
top level: Suffix
direction: direction/forward
start: 1
end: 21
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation1934226
display id: annotation1934226
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934226/range1934226/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_G00001/annotation1934226/range1934226/1
type: Range
persistent identity: range1934226
display id: range1934226
version: 1
top level: Suffix
direction: direction/forward
start: 2
end: 7
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1934228
display id: annotation1934228
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934228/range1934228/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00001/annotation1934228/range1934228/1
type: Range
persistent identity: range1934228
display id: range1934228
version: 1
top level: Suffix
direction: direction/forward
start: 9
end: 16
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1934227
display id: annotation1934227
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934227/range1934227/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00001/annotation1934227/range1934227/1
type: Range
persistent identity: range1934227
display id: range1934227
version: 1
top level: Suffix
direction: direction/forward
start: 16
end: 21
orientation: inline

VF2
type: ComponentDefinition
persistent identity: BBa G00100
display id: BBa_G00100
version: 1
was generated by: iGEM to SBOL conversion
description: Forward primer for sequencing/amplifying BioBrick parts (VF2)
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Tom Knight
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence annotation: VF2
sequence: https://synbiohub.org/public/igem/BBa_G00100_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation1934505
display id: annotation1934505
version: 1
top level: VF2
location: https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1
type: Range
persistent identity: range1934505
display id: range1934505
version: 1
top level: VF2
direction: direction/forward
start: 1
end: 20
orientation: inline

VR
type: ComponentDefinition
persistent identity: BBa G00102
display id: BBa_G00102
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse BioBrick primer annealing site (VR binding site)
created: 2006-02-03T12:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_G00102_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation1961229
display id: annotation1961229
version: 1
top level: VR
location: https://synbiohub.org/public/igem/BBa_G00102/annotation1961229/range1961229/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_G00102/annotation1961229/range1961229/1
type: Range
persistent identity: range1961229
display id: range1961229
version: 1
top level: VR
direction: direction/reverse
start: 1
end: 20
orientation: inline

FRT
type: ComponentDefinition
persistent identity: BBa J72001
display id: BBa_J72001
version: 1
was generated by: iGEM to SBOL conversion
description: {FRT} recombination site for flp recombinase in BBb
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:08:26Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: false
experience: experience/None
group u list: _171_
m user id: 0
owner id: 483
owning group id: 171
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J72001_sequence/1

aspA(rev)
type: ComponentDefinition
persistent identity: BBa J107059
display id: BBa_J107059
version: 1
was generated by: iGEM to SBOL conversion
description: aspA coding sequence (reverse)
created: 2012-08-25T11:00:00Z
modified: 2015-08-31T04:08:25Z
mutable provenance: Same as <partinfo>BBa_C0083</partinfo>.
owned by: james
owned by: myers
mutable description: This is <partinfo>BBa_C0083</partinfo> reversed.
discontinued: false
dominant: false
experience: experience/None
group u list: _587_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: BioBrick format.
star: false
creator: Lorenzo Pasotti
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J107059_sequence/1

scar
type: ComponentDefinition
persistent identity: BBa J107060
display id: BBa_J107060
version: 1
was generated by: iGEM to SBOL conversion
description: XbaI/NheI mixed site without the initial CT nucleotides
created: 2012-08-25T11:00:00Z
modified: 2015-08-31T04:08:25Z
mutable provenance: Registry.
owned by: james
owned by: myers
mutable description: It is the same as <partinfo>BBa_G0006</partinfo>, but it does not have the initial CT nucleotides. It is useful to submit XbaI/NheI fusions where the BioBrick with XbaI is a coding sequence (so XbaI is TCTAG instead of TCTAGAG).
discontinued: false
dominant: false
experience: experience/None
group u list: _587_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Digested XbaI ligated with digested NheI.
star: false
creator: Lorenzo Pasotti
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J107060_sequence/1

scar
type: ComponentDefinition
persistent identity: BBa G0005
display id: BBa_G0005
version: 1
was generated by: iGEM to SBOL conversion
description: NheI/SpeI mixed site
created: 2007-02-26T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: NheI and SpeI
owned by: james
owned by: myers
mutable description: NheI/SpeI mixed site
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0005_sequence/1

CmR
type: ComponentDefinition
persistent identity: BBa K300999
display id: BBa_K300999
version: 1
was generated by: iGEM to SBOL conversion
description: chloramphenicol resistance cassette (rev)
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: <partinfo>BBa_P1004</partinfo>
owned by: james
owned by: myers
mutable description: Reverse complement of <partinfo>BBa_P1004</partinfo> (chloramphenicol resistance cassette without transcriptional terminator).
discontinued: false
dominant: false
experience: experience/Works
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: see <partinfo>BBa_P1004</partinfo> for detailed information.
star: false
creator: Lorenzo Pasotti, Manuel Lupotto, Paolo Magni
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: cmR(rev)
sequence: https://synbiohub.org/public/igem/BBa_K300999_sequence/1

cmR(rev)
type: SequenceAnnotation
persistent identity: annotation2081291
display id: annotation2081291
version: 1
top level: CmR
location: https://synbiohub.org/public/igem/BBa_K300999/annotation2081291/range2081291/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K300999/annotation2081291/range2081291/1
type: Range
persistent identity: range2081291
display id: range2081291
version: 1
top level: CmR
direction: direction/reverse
start: 1
end: 663
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa K300995
display id: BBa_K300995
version: 1
was generated by: iGEM to SBOL conversion
description: AvrII/XbaI mixed site (when XbaI comes from RFC10 Prefix) - rev
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: Reverse complement of <partinfo>BBa_K300997</partinfo>
discontinued: false
dominant: false
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: See <partinfo>BBa_K300997</partinfo>
star: false
creator: Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K300995_sequence/1

scar
type: ComponentDefinition
persistent identity: BBa G0001
display id: BBa_G0001
version: 1
was generated by: iGEM to SBOL conversion
description: XbaI/SpeI mixed site, reverse
created: 2007-02-26T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: XbaI and SpeI
owned by: james
owned by: myers
mutable description: XbaI/SpeI mixed site, reverse
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_G0001_sequence/1

BBa_B0062
type: ComponentDefinition
persistent identity: BBa B0062
display id: BBa_B0062
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (Reverse B0052)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:21Z
owned by: james
owned by: myers
mutable description: Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0062_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318637
display id: annotation318637
version: 1
top level: BBa_B0062
location: https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1
type: Range
persistent identity: range318637
display id: range318637
version: 1
top level: BBa_B0062
direction: direction/forward
start: 14
end: 33
orientation: inline

R6K ori
type: ComponentDefinition
persistent identity: BBa K300998
display id: BBa_K300998
version: 1
was generated by: iGEM to SBOL conversion
description: [R6K] Origin of replication (rev)
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: See <partinfo>BBa_J61001</partinfo> for detailed information.
owned by: james
owned by: myers
mutable description: Reverse complement of <partinfo>BBa_J61001</partinfo> (R6K conditional replication origin).
discontinued: false
dominant: false
experience: experience/Works
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: <partinfo>BBa_J61001</partinfo>
star: false
creator: Lorenzo Pasotti, Manuel Lupotto, Paolo Magni
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K300998_sequence/1

scar
type: ComponentDefinition
persistent identity: BBa K300994
display id: BBa_K300994
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI/AvrII mixed site (when SpeI comes from RFC10 Prefix) - rev
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: Reverse complement of <partinfo>BBa_K300996</partinfo>.
discontinued: false
dominant: false
experience: experience/None
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: See <partinfo>BBa_K300996</partinfo>
star: false
creator: Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K300994_sequence/1

https://synbiohub.org/public/igem/BBa_J107058_sequence/1
type: Sequence
persistent identity: BBa J107058 sequence
display id: BBa_J107058_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgcgaagttcctatactttctagagaataggaacttccctagtagcagttcaacctgttgatagtacgtactaagctctcatgtttcacgtactaagctctcatgtttaacgtactaagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaacaataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctcaaagcaattttgagtgacacaggaacacttaacggctgacatgggaattagccatgggcccgtgcgaatcactctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtattattacgccccgccctgccactcatcacagtactgttgtaattcattaagcatgcggccgacatggaagccatcacaaacggcatgatgaacctggatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatcgtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacaaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtggagaaactgccggaaatcgtcgtggtattcactccagagggacgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccagatcaccagctcaccgtctttcatggccatacgaaactccgggtgagcgttcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatcccgtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaacctctagggcgaagttcctatactttctagagaataggaacttcgctagtattattaagctactaaagcgtagttttcgtcgtttgcagcctgttcgctttcatcagtatagcgttttgctttgtaagccgggtgcatcagattctgtacggagaaaatatcgtcaagttccgcttcagtcaacagaccgcgttccagaacgacttcacgtacactcttaccggtttcggcacagattttacccacgatgtcaccgttgtggtgaccgatgaacgggttcaggtaagtaacgataccgatagagttgtaaacgtaaccttcgcacacttctttgttagcagtgatgccgttaatgcatttttccagcaggttgtagcaagcgttggtcagaatgtgaacggattcgaacatggcctggccaatgaccggctccataacgttcaattgcagctgacctgcttctgctgccatggtaacagtggtgtcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0025_sequence/1
type: Sequence
persistent identity: BBa B0025 sequence
display id: BBa_B0025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0053_sequence/1
type: Sequence
persistent identity: BBa B0053 sequence
display id: BBa_B0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0054_sequence/1
type: Sequence
persistent identity: BBa B0054 sequence
display id: BBa_B0054_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0055_sequence/1
type: Sequence
persistent identity: BBa B0055 sequence
display id: BBa_B0055_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00000_sequence/1
type: Sequence
persistent identity: BBa G00000 sequence
display id: BBa_G00000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0062_sequence/1
type: Sequence
persistent identity: BBa B0062 sequence
display id: BBa_B0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagataaaaaaaatccttagctttcgctaaggatgatttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00001_sequence/1
type: Sequence
persistent identity: BBa G00001 sequence
display id: BBa_G00001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00100_sequence/1
type: Sequence
persistent identity: BBa G00100 sequence
display id: BBa_G00100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgccacctgacgtctaagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00102_sequence/1
type: Sequence
persistent identity: BBa G00102 sequence
display id: BBa_G00102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctcactcaaaggcggtaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J72001_sequence/1
type: Sequence
persistent identity: BBa J72001 sequence
display id: BBa_J72001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgaagttcctatactttctagagaataggaacttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J107059_sequence/1
type: Sequence
persistent identity: BBa J107059 sequence
display id: BBa_J107059_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattaagctactaaagcgtagttttcgtcgtttgcagcctgttcgctttcatcagtatagcgttttgctttgtaagccgggtgcatcagattctgtacggagaaaatatcgtcaagttccgcttcagtcaacagaccgcgttccagaacgacttcacgtacactcttaccggtttcggcacagattttacccacgatgtcaccgttgtggtgaccgatgaacgggttcaggtaagtaacgataccgatagagttgtaaacgtaaccttcgcacacttctttgttagcagtgatgccgttaatgcatttttccagcaggttgtagcaagcgttggtcagaatgtgaacggattcgaacatggcctggccaatgaccggctccataacgttcaattgcagctgacctgcttctgctgccatggtaacagtggtgtcgttaccgatgactttgaagcatacctggttaaccacttccggaacaaccgggtttactttagctggcatgatggaagagcccgcttgcagttccggcaggttgatctcgttcaggccggcacgtgggcctgaagagagcaagcgcaggtcgttacagattttggacatcttcacagccaggcgtttcagcgcgccgtgaaccataacataagcgccgcagtcagaggtcgcttcgatcaggtcttcagccggtacgcatgggaagccagtaacttcagccagttttttcactgccagcggagagtactctttcggcgtgttcagaccagtaccgattgctgttgcaccaaggttaacttccagcagcagttcagcggtacgttggatgtttttcacttcttctttcagcaggatgctgaaagcgcgaaattcctgaccgagggtcatcggtactgcgtcttgcagctgggtacgacccattttcaggatgtcctgaaattcgacagctttacgttcaaagccttcacgcagttggttaatcgcatctaccagcttaatcagggaagagtaaactgcgatacggaaaccggtcgggtaggcgtcgttagtggactgacatttgttaacatggtcgttcgggttcaggtactgatattcacctttttggtgacccatcagttccagaccgatattggccagcacttcgttggtgttcatgtttacggaagtacctgcgccgccctggtagacgtctaccgggaactgatccatgcattttccgttgttcaggacttcatcacatgcggcaatgatggcattcgctacacttttaggaatggtttgcagctctttgtttgccatagctgcggcttttttaaccattaccataccgcgaacaaattcaggaatatcactgattttgttgttgctgatatagaagttttcaatcgctctcagagtgtgaacaccatagtaggcatcagctggaacttccctggtacccaacagatcttcttcgatacgaatgttgtttgacatgtgaaccttctttttcaagctgccaatgatttgctttaaacacat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J107060_sequence/1
type: Sequence
persistent identity: BBa J107060 sequence
display id: BBa_J107060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0005_sequence/1
type: Sequence
persistent identity: BBa G0005 sequence
display id: BBa_G0005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctagta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300999_sequence/1
type: Sequence
persistent identity: BBa K300999 sequence
display id: BBa_K300999_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattacgccccgccctgccactcatcacagtactgttgtaattcattaagcatgcggccgacatggaagccatcacaaacggcatgatgaacctggatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatcgtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacaaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtggagaaactgccggaaatcgtcgtggtattcactccagagggacgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccagatcaccagctcaccgtctttcatggccatacgaaactccgggtgagcgttcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatcccgtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300998_sequence/1
type: Sequence
persistent identity: BBa K300998 sequence
display id: BBa_K300998_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcagttcaacctgttgatagtacgtactaagctctcatgtttcacgtactaagctctcatgtttaacgtactaagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaacaataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctcaaagcaattttgagtgacacaggaacacttaacggctgacatgggaattagccatgggcccgtgcgaatca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300995_sequence/1
type: Sequence
persistent identity: BBa K300995 sequence
display id: BBa_K300995_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctctagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300994_sequence/1
type: Sequence
persistent identity: BBa K300994 sequence
display id: BBa_K300994_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctagta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0001_sequence/1
type: Sequence
persistent identity: BBa G0001 sequence
display id: BBa_G0001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctctagta
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z