BioBrick integrative vector targeting aspA locus
<partinfo>BBa_K300000</partinfo> specialized to target aspA locus.
It has been derived from <partinfo>BBa_K300000</partinfo> base vector by exciding the default integration guide (attP of phi80 phage) and ligating aspA (<partinfo>BBa_C0083</partinfo>) as guide.
It can be propagated in strains with pir or pir-116 locus. Integration works only in host strains with an intact aspA genomic locus.
Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
identical to E. coli MG1655 between tonB and yciA
Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
This part is the standard BioBricks prefix.
This part is the standard BioBricks suffix.
Forward primer for sequencing/amplifying BioBrick parts (VF2)
Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
Reverse BioBrick primer annealing site (VR binding site)
This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101.
{FRT} recombination site for flp recombinase in BBb
This is <partinfo>BBa_C0083</partinfo> reversed.
XbaI/NheI mixed site without the initial CT nucleotides
It is the same as <partinfo>BBa_G0006</partinfo>, but it does not have the initial CT nucleotides. It is useful to submit XbaI/NheI fusions where the BioBrick with XbaI is a coding sequence (so XbaI is TCTAG instead of TCTAGAG).
Digested XbaI ligated with digested NheI.
chloramphenicol resistance cassette (rev)
Reverse complement of <partinfo>BBa_P1004</partinfo> (chloramphenicol resistance cassette without transcriptional terminator).
see <partinfo>BBa_P1004</partinfo> for detailed information.
Lorenzo Pasotti, Manuel Lupotto, Paolo Magni
AvrII/XbaI mixed site (when XbaI comes from RFC10 Prefix) - rev
Reverse complement of <partinfo>BBa_K300997</partinfo>
Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni
Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
See <partinfo>BBa_J61001</partinfo> for detailed information.
Reverse complement of <partinfo>BBa_J61001</partinfo> (R6K conditional replication origin).
SpeI/AvrII mixed site (when SpeI comes from RFC10 Prefix) - rev
Reverse complement of <partinfo>BBa_K300996</partinfo>.
Conversion of the iGEM parts registry to SBOL2.1