LOCUS BBa_J107077 3886 bp DNA linear 09-May-2024 DEFINITION pBBknock standard vector for allelic replacement ACCESSION BBa_J107077 VERSION BBa_J107077.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 2..7 /label=SpeI misc_feature 16..21 /label=PstI misc_feature 9..16 /label=NotI misc_feature 1..21 /label=BBa_G00001 promoter 22..56 /label=BBa_J23101 misc_feature 57..64 /label=BBa_G0002 RBS 78..83 /label=rbs CDS 91..1014 /label=cds misc_feature 65..1022 /label=BBa_J33204 misc_feature 1023..1028 /label=BBa_M31985 terminator 1029..1077 /label=BBa_J107113 misc_feature 1078..1083 /label=HindIII misc_feature 1078..1083 /label=BBa_M300055 misc_feature 1084..3026 /label=BBa_J107112 misc_feature 3027..3032 /label=NsiI site misc_feature 3027..3032 /label=BBa_B0046 terminator 3033..3089 /label=BBa_J107114 CDS 3090..3095 /label=BBa_I766205 CDS 3096..3758 /label=cmR(rev) misc_feature 3096..3864 /label=BBa_K300999 misc_feature 3865..3870 /label=EcoRI site misc_feature 3880..3885 /label=XbaI site misc_feature 3865..3886 /label=Prefix misc_feature 3871..3878 /label=NotI site misc_feature 3865..3886 /label=BBa_G00000 ORIGIN 1 tactagtagc ggccgctgca gtttacagct agctcagtcc taggtattat gctagctact 61 agagctcatg aactatgaag aggtgacgtc atgaacaaag gtgtaatgcg accgggccat 121 gtgcagctgc gtgtactgga catgagcaag gccctggaac actacgtcga gttgctgggc 181 ctgatcgaga tggaccgtga cgaccagggc cgtgtctatc tgaaggcttg gaccgaagtg 241 gataagtttt ccctggtgct acgcgaggct gacgagccgg gcatggattt tatgggtttc 301 aaggttgtgg atgaggatgc tctccggcaa ctggagcggg atctgatggc atatggctgt 361 gccgttgagc agctacccgc aggtgaactg aacagttgtg gccggcgcgt gcgcttccag 421 gccccctccg ggcatcactt cgagttgtat gcagacaagg aatatactgg aaagtggggt 481 ttgaatgacg tcaatcccga ggcatggccg cgcgatctga aaggtatggc ggctgtgcgt 541 ttcgaccacg ccctcatgta tggcgacgaa ttgccggcga cctatgacct gttcaccaag 601 gtgctcggtt tctatctggc cgaacaggtg ctggacgaaa atggcacgcg cgtcgcccag 661 tttctcagtc tgtcgaccaa ggcccacgac gtggccttca ttcaccatcc ggaaaaaggc 721 cgcctccatc atgtgtcctt ccacctcgaa acctgggaag acttgcttcg cgccgccgac 781 ctgatctcca tgaccgacac atctatcgat atcggcccaa cccgccacgg cctcactcac 841 ggcaagacca tctacttctt cgacccgtcc ggtaaccgca acgaagtgtt ctgcggggga 901 gattacaact acccggacca caaaccggtg acctggacca ccgaccagct gggcaaggcg 961 atcttttacc acgaccgcat tctcaacgaa cgattcatga ccgtgctgac ctgatggtcc 1021 ggagatctcc aattattgaa ggccgctaac gcggcctttt tttgtttctg gtctcccaag 1081 cttgtatgga cagttttccc tttgatatgt aacggtgaac agttgttcta cttttgtttg 1141 ttagtcttga tgcttcactg atagatacaa gagccataag aacctcagat ccttccgtat 1201 ttagccagta tgttctctag tgtggttcgt tgtttttgcg tgagccatga gaacgaacca 1261 ttgagatcat acttactttg catgtcactc aaaaattttg cctcaaaact ggtgagctga 1321 atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc cgttatgtag gtaggaatct 1381 gatgtaatgg ttgttggtat tttgtcacca ttcattttta tctggttgtt ctcaagttcg 1441 gttacgagat ccatttgtct atctagttca acttggaaaa tcaacgtatc agtcgggcgg 1501 cctcgcttat caaccaccaa tttcatattg ctgtaagtgt ttaaatcttt acttattggt 1561 ttcaaaaccc attggttaag ccttttaaac tcatggtagt tattttcaag cattaacatg 1621 aacttaaatt catcaaggct aatctctata tttgccttgt gagttttctt ttgtgttagt 1681 tcttttaata accactcata aatcctcata gagtatttgt tttcaaaaga cttaacatgt 1741 tccagattat attttatgaa tttttttaac tggaaaagat aaggcaatat ctcttcacta 1801 aaaactaatt ctaatttttc gcttgagaac ttggcatagt ttgtccactg gaaaatctca 1861 aagcctttaa ccaaaggatt cctgatttcc acagttctcg tcatcagctc tctggttgct 1921 ttagctaata caccataagc attttcccta ctgatgttca tcatctgaac gtattggtta 1981 taagtgaacg ataccgtccg ttctttcctt gtagggtttt caatcgtggg gttgagtagt 2041 gccacacagc ataaaattag cttggtttca tgctccgtta agtcatagcg actaatcgct 2101 agttcatttg ctttgaaaac aactaattca gacatacatc tcaattggtc taggtgattt 2161 taatcactat accaattgag atgggctagt caatgataat tacatgtcct tttcctttga 2221 gttgtgggta tctgtaaatt ctgctagacc tttgctggaa aacttgtaaa ttctgctaga 2281 ccctctgtaa attccgctag acctttgtgt gttttttttg tttatattca agtggttata 2341 atttatagaa taaagaaaga ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat 2401 aactcactac tttagtcagt tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct 2461 cctctacaaa acagacctta aaaccctaaa ggcttaagta gcaccctcgc aagctcggtt 2521 gcggccgcaa tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga 2581 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg 2641 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag 2701 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct 2761 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta 2821 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaacg 2881 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 2941 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 3001 atatatgagt aaacttggtc tgacagatgc atggaccgaa aaataaaaag acgcttttca 3061 gcgtcttttt atttttcttt ggtaccgagc tcgagttatt acgccccgcc ctgccactca 3121 tcacagtact gttgtaattc attaagcatg cggccgacat ggaagccatc acaaacggca 3181 tgatgaacct ggatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc 3241 atcgtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg 3301 aaactcaccc agggattggc tgagacaaaa aacatattct caataaaccc tttagggaaa 3361 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtggag aaactgccgg 3421 aaatcgtcgt ggtattcact ccagagggac gaaaacgttt cagtttgctc atggaaaacg 3481 gtgtaacaag ggtgaacact atcccagatc accagctcac cgtctttcat ggccatacga 3541 aactccgggt gagcgttcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg 3601 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta 3661 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat 3721 atatcaacgg tggtatatcc cgtgattttt ttctccattt tagcttcctt agctcctgaa 3781 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg 3841 gaacctctta cgtgcccgat caacgaattc gcggccgctt ctagag //