pBBknock
type: ComponentDefinition
persistent identity: BBa J107077
display id: BBa_J107077
version: 1
was generated by: iGEM to SBOL conversion
description: pBBknock standard vector for allelic replacement
created: 2016-01-02T12:00:00Z
modified: 2016-02-11T02:50:44Z
mutable provenance: Synthetic DNA obtained via GenScript synthesis service.
owned by: james
owned by: myers
mutable description: This vector can be specialized to target the desired locus in the Escherichia coli genome and carry out allelic replacement, e.g., knockout, chromosomal integration, mutagenesis, etc. See Casanova et al. (2016), Biol Proced Online for detailed instructions and protocols.
discontinued: false
dominant: false
experience: experience/Works
group u list: _587_
m user id: 2621
owner id: 2621
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: See BBa_J107112.
star: false
creator: Michela Casanova, Lorenzo Pasotti, Susanna Zucca, Nicolo Politi, Ilaria Massaiu, Cinzia Calvio, Gabriella Cusella, Paolo Magni
type: DnaRegion
role: partType/Plasmid Backbone
role: SO:0000755
component: BBa_G00001
component: BBa_M31985
component: BBa_J33204
component: BBa_G0002
component: BBa_J23101
component: BBa_B0046
component: BBa_J107112
component: BBa_M300055
component: BBa_J107113
component: BBa_G00000
component: BBa_K300999
component: BBa_I766205
component: BBa_J107114
sequence annotation: BBa_G00001
sequence annotation: BBa_J23101
sequence annotation: BBa_G0002
sequence annotation: BBa_J33204
sequence annotation: BBa_M31985
sequence annotation: BBa_J107113
sequence annotation: BBa_M300055
sequence annotation: BBa_J107112
sequence annotation: BBa_B0046
sequence annotation: BBa_J107114
sequence annotation: BBa_I766205
sequence annotation: BBa_K300999
sequence annotation: BBa_G00000
sequence: https://synbiohub.org/public/igem/BBa_J107077_sequence/1

BBa_G00001
type: Component
persistent identity: component2478496
display id: component2478496
version: 1
top level: pBBknock
definition: Suffix
access: public

BBa_M31985
type: Component
persistent identity: component2478502
display id: component2478502
version: 1
top level: pBBknock
definition: BglII
access: public

BBa_J33204
type: Component
persistent identity: component2478501
display id: component2478501
version: 1
top level: pBBknock
definition: BBa_J33204
access: public

BBa_G0002
type: Component
persistent identity: component2478498
display id: component2478498
version: 1
top level: pBBknock
definition: SX scar
access: public

BBa_J23101
type: Component
persistent identity: component2478497
display id: component2478497
version: 1
top level: pBBknock
definition: BBa_J23101
access: public

BBa_B0046
type: Component
persistent identity: component2478508
display id: component2478508
version: 1
top level: pBBknock
definition: NsiI
access: public

BBa_J107112
type: Component
persistent identity: component2478506
display id: component2478506
version: 1
top level: pBBknock
definition: pSC101ts
access: public

BBa_M300055
type: Component
persistent identity: component2478505
display id: component2478505
version: 1
top level: pBBknock
definition: BBa_M300055
access: public

BBa_J107113
type: Component
persistent identity: component2478503
display id: component2478503
version: 1
top level: pBBknock
definition: BBa_J107113
access: public

BBa_G00000
type: Component
persistent identity: component2478517
display id: component2478517
version: 1
top level: pBBknock
definition: Prefix
access: public

BBa_K300999
type: Component
persistent identity: component2478512
display id: component2478512
version: 1
top level: pBBknock
definition: CmR
access: public

BBa_I766205
type: Component
persistent identity: component2478510
display id: component2478510
version: 1
top level: pBBknock
definition: BBa_I766205
access: public

BBa_J107114
type: Component
persistent identity: component2478509
display id: component2478509
version: 1
top level: pBBknock
definition: BBa_J107114
access: public

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2478496
display id: annotation2478496
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478496/range2478496/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_J107077/annotation2478496/range2478496/1
type: Range
persistent identity: range2478496
display id: range2478496
version: 1
top level: pBBknock
direction: direction/reverse
start: 1
end: 21
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2478497
display id: annotation2478497
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478497/range2478497/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_J107077/annotation2478497/range2478497/1
type: Range
persistent identity: range2478497
display id: range2478497
version: 1
top level: pBBknock
direction: direction/reverse
start: 22
end: 56
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2478498
display id: annotation2478498
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478498/range2478498/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_J107077/annotation2478498/range2478498/1
type: Range
persistent identity: range2478498
display id: range2478498
version: 1
top level: pBBknock
direction: direction/reverse
start: 57
end: 64
orientation: inline

BBa_J33204
type: SequenceAnnotation
persistent identity: annotation2478501
display id: annotation2478501
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478501/range2478501/1
component: BBa_J33204

https://synbiohub.org/public/igem/BBa_J107077/annotation2478501/range2478501/1
type: Range
persistent identity: range2478501
display id: range2478501
version: 1
top level: pBBknock
direction: direction/reverse
start: 65
end: 1022
orientation: inline

BBa_M31985
type: SequenceAnnotation
persistent identity: annotation2478502
display id: annotation2478502
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478502/range2478502/1
component: BBa_M31985

https://synbiohub.org/public/igem/BBa_J107077/annotation2478502/range2478502/1
type: Range
persistent identity: range2478502
display id: range2478502
version: 1
top level: pBBknock
direction: direction/reverse
start: 1023
end: 1028
orientation: inline

BBa_J107113
type: SequenceAnnotation
persistent identity: annotation2478503
display id: annotation2478503
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478503/range2478503/1
component: BBa_J107113

https://synbiohub.org/public/igem/BBa_J107077/annotation2478503/range2478503/1
type: Range
persistent identity: range2478503
display id: range2478503
version: 1
top level: pBBknock
direction: direction/reverse
start: 1029
end: 1077
orientation: inline

BBa_M300055
type: SequenceAnnotation
persistent identity: annotation2478505
display id: annotation2478505
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478505/range2478505/1
component: BBa_M300055

https://synbiohub.org/public/igem/BBa_J107077/annotation2478505/range2478505/1
type: Range
persistent identity: range2478505
display id: range2478505
version: 1
top level: pBBknock
direction: direction/reverse
start: 1078
end: 1083
orientation: inline

BBa_J107112
type: SequenceAnnotation
persistent identity: annotation2478506
display id: annotation2478506
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478506/range2478506/1
component: BBa_J107112

https://synbiohub.org/public/igem/BBa_J107077/annotation2478506/range2478506/1
type: Range
persistent identity: range2478506
display id: range2478506
version: 1
top level: pBBknock
direction: direction/reverse
start: 1084
end: 3026
orientation: inline

BBa_B0046
type: SequenceAnnotation
persistent identity: annotation2478508
display id: annotation2478508
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478508/range2478508/1
component: BBa_B0046

https://synbiohub.org/public/igem/BBa_J107077/annotation2478508/range2478508/1
type: Range
persistent identity: range2478508
display id: range2478508
version: 1
top level: pBBknock
direction: direction/reverse
start: 3027
end: 3032
orientation: inline

BBa_J107114
type: SequenceAnnotation
persistent identity: annotation2478509
display id: annotation2478509
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478509/range2478509/1
component: BBa_J107114

https://synbiohub.org/public/igem/BBa_J107077/annotation2478509/range2478509/1
type: Range
persistent identity: range2478509
display id: range2478509
version: 1
top level: pBBknock
direction: direction/reverse
start: 3033
end: 3089
orientation: inline

BBa_I766205
type: SequenceAnnotation
persistent identity: annotation2478510
display id: annotation2478510
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478510/range2478510/1
component: BBa_I766205

https://synbiohub.org/public/igem/BBa_J107077/annotation2478510/range2478510/1
type: Range
persistent identity: range2478510
display id: range2478510
version: 1
top level: pBBknock
direction: direction/reverse
start: 3090
end: 3095
orientation: inline

BBa_K300999
type: SequenceAnnotation
persistent identity: annotation2478512
display id: annotation2478512
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478512/range2478512/1
component: BBa_K300999

https://synbiohub.org/public/igem/BBa_J107077/annotation2478512/range2478512/1
type: Range
persistent identity: range2478512
display id: range2478512
version: 1
top level: pBBknock
direction: direction/reverse
start: 3096
end: 3864
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2478517
display id: annotation2478517
version: 1
top level: pBBknock
location: https://synbiohub.org/public/igem/BBa_J107077/annotation2478517/range2478517/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_J107077/annotation2478517/range2478517/1
type: Range
persistent identity: range2478517
display id: range2478517
version: 1
top level: pBBknock
direction: direction/reverse
start: 3865
end: 3886
orientation: inline

BBa_J107113
type: ComponentDefinition
persistent identity: BBa J107113
display id: BBa_J107113
version: 1
was generated by: iGEM to SBOL conversion
description: L3S3P22 terminator
created: 2016-01-02T12:00:00Z
modified: 2016-01-02T08:54:00Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: HindIII
discontinued: false
dominant: false
experience: experience/None
group u list: _587_
m user id: 2621
owner id: 2621
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Lorenzo Pasotti
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_J107113_sequence/1

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

Suffix
type: ComponentDefinition
persistent identity: BBa G00001
display id: BBa_G00001
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick cloning site suffix
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: This part is the standard BioBricks suffix.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Suffix
sequence annotation: SpeI
sequence annotation: NotI
sequence annotation: PstI
sequence: https://synbiohub.org/public/igem/BBa_G00001_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation1821544
display id: annotation1821544
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1821544/range1821544/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_G00001/annotation1821544/range1821544/1
type: Range
persistent identity: range1821544
display id: range1821544
version: 1
top level: Suffix
direction: direction/forward
start: 1
end: 21
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation1934226
display id: annotation1934226
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934226/range1934226/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_G00001/annotation1934226/range1934226/1
type: Range
persistent identity: range1934226
display id: range1934226
version: 1
top level: Suffix
direction: direction/forward
start: 2
end: 7
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1934228
display id: annotation1934228
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934228/range1934228/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00001/annotation1934228/range1934228/1
type: Range
persistent identity: range1934228
display id: range1934228
version: 1
top level: Suffix
direction: direction/forward
start: 9
end: 16
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1934227
display id: annotation1934227
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934227/range1934227/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00001/annotation1934227/range1934227/1
type: Range
persistent identity: range1934227
display id: range1934227
version: 1
top level: Suffix
direction: direction/forward
start: 16
end: 21
orientation: inline

SX scar
type: ComponentDefinition
persistent identity: BBa G0002
display id: BBa_G0002
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI/XbaI mixed site
created: 2007-02-26T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: XbaI and SpeI sites
owned by: james
owned by: myers
mutable description: XbaI/SpeI mixed site. Simply used to aid in entry of parts into the registry.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0002_sequence/1

BBa_J33204
type: ComponentDefinition
persistent identity: BBa J33204
display id: BBa_J33204
version: 1
was generated by: iGEM to SBOL conversion
description: xylE reporter gene with rbs
created: 2006-10-16T11:00:00Z
modified: 2015-08-31T04:08:46Z
mutable provenance: The template DNA was kindly supplied by Dr. Peter Williams of the University of Wales, Bangor. The primer design was based on Genbank sequence M64747 (GI:151718). The sequence reported here was confirmed by sequencing the Biobrick construct.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part includes the xylE gene from the Pseudomonas putida TOL (naphthalene and xylene degradadative plasmid) pWW0. This gene encodes the enzyme catechol-2,3-dioxygenase (metapyrocatechase), which converts catechol to the bright yellow product 2-hydroxy-cis,cis-muconic semialdehyde. This is a useful reporter gene; colonies or broths expressing active XylE, in the presence of oxygen, will rapidly convert catechol, a cheap colourless substrate, to a bright yellow compound with an absorbance maximum around 377 nm. The part includes the native ribosome binding site, so simply needs to be added after a suitable promoter to act as a reporter. I have previously used this gene to generate whole cell biosensors for various heavy metals. Note that, unlike Xgal etc., catechol in solution is prone to spontaneous oxidation resulting in brown melanin-like polymeric products, so is not stable enough to incorporate into plates or growth media; it should be dripped onto colonies (I use 10 mM catechol in water for this) or added to liquid cultures at a final concentration of about 0.5 mM prior to assay. Note also that there is a SacI site at the very start of the part, when the prefix is included, so this can be used as a replacement vector to introduce PCR products with SacI-SpeI ends into pSB1A2 giving them full Biobrick prefixes and suffixes (but don't forget the G base before the SpeI site). This results in shorter non-complementary tails on PCR primers than using a full prefix or suffix. You can test for colonies that have lost xylE using catechol as described above.
discontinued: false
dominant: false
experience: experience/Works
group u list: _63_
m user id: 0
owner id: 837
owning group id: 63
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Note that this sequence includes the native ribsomome binding site. Also, when the prefix is included, there is a SacI site at the start, which allows this part to be used as a vector for insertion of PCR products with SacI-SpeI ends into pSB1A2, replacing xylE, giving the full Biobrick prefixes and suffixes (but don't forget the G base before the SpeI site). This results in shorter non-complementary tails on PCR primers than using a full prefix or suffix. You can test for colonies that have lost xylE using catechol as described above.
star: true
creator: Chris French
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: rbs
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_J33204_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1903406
display id: annotation1903406
version: 1
top level: BBa_J33204
location: https://synbiohub.org/public/igem/BBa_J33204/annotation1903406/range1903406/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J33204/annotation1903406/range1903406/1
type: Range
persistent identity: range1903406
display id: range1903406
version: 1
top level: BBa_J33204
direction: direction/forward
start: 14
end: 19
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation1903407
display id: annotation1903407
version: 1
top level: BBa_J33204
location: https://synbiohub.org/public/igem/BBa_J33204/annotation1903407/range1903407/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J33204/annotation1903407/range1903407/1
type: Range
persistent identity: range1903407
display id: range1903407
version: 1
top level: BBa_J33204
direction: direction/forward
start: 27
end: 950
orientation: inline

BglII
type: ComponentDefinition
persistent identity: BBa M31985
display id: BBa_M31985
version: 1
was generated by: iGEM to SBOL conversion
description: BglII restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: BglII restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31985_sequence/1

pSC101ts
type: ComponentDefinition
persistent identity: BBa J107112
display id: BBa_J107112
version: 1
was generated by: iGEM to SBOL conversion
description: pSC101ts origin with SpeI removed
created: 2016-01-02T12:00:00Z
modified: 2016-01-02T08:10:54Z
mutable provenance: pAH123 (reference: doi 10.1128/JB.183.21.6384-6393.2001).
owned by: james
owned by: myers
mutable description: Temperature-sensitive pSC101 replication origin without SpeI site. This part was used to design the pBBknock BioBrick vector for allelic replacement (BBa_J107077).
discontinued: false
dominant: false
experience: experience/Works
group u list: _587_
m user id: 2621
owner id: 2621
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The origin has been retrieved from pAH123 vector [GenBank: AY048726]. The sequence was extended to the upstream NcoI restriction site and to the downstream stop codon of the ampicillin resistance gene in pAH123. We also modified the origin region by removing the SpeI site, according to the modification present in the available sequence of pSC101 (BBa_I50042), to ensure that the nucleotide changes do not affect the replication origin functioning, e.g., in the widely used pSB4C5 vector. The sequence of pAH123 was obtained via DNA sequencing, since its submitted DNA sequence [GenBank: AY048726] contains errors, e.g., in the temperature-sensitive mutation. The read sequence was then modified to remove SpeI site.
star: false
creator: Lorenzo Pasotti
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_J107112_sequence/1

BBa_J107114
type: ComponentDefinition
persistent identity: BBa J107114
display id: BBa_J107114
version: 1
was generated by: iGEM to SBOL conversion
description: L3S2P42 terminator (reverse)
created: 2016-01-02T12:00:00Z
modified: 2016-01-02T08:55:00Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: Transcriptional terminator (reverse).
discontinued: false
dominant: false
group u list: _587_
m user id: 2621
owner id: 2621
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Lorenzo Pasotti
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_J107114_sequence/1

BBa_M300055
type: ComponentDefinition
persistent identity: BBa M300055
display id: BBa_M300055
version: 1
was generated by: iGEM to SBOL conversion
description: hind3
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:13:59Z
mutable provenance: m13k07
owned by: james
owned by: myers
mutable description: restriction enzyme
discontinued: false
dominant: true
group u list: _102_
m user id: 0
owner id: 1384
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: see others
star: false
creator: Jennifer Chao
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: HindIII
sequence: https://synbiohub.org/public/igem/BBa_M300055_sequence/1

HindIII
type: SequenceAnnotation
persistent identity: annotation1917479
display id: annotation1917479
version: 1
top level: BBa_M300055
location: https://synbiohub.org/public/igem/BBa_M300055/annotation1917479/range1917479/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M300055/annotation1917479/range1917479/1
type: Range
persistent identity: range1917479
display id: range1917479
version: 1
top level: BBa_M300055
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_I766205
type: ComponentDefinition
persistent identity: BBa I766205
display id: BBa_I766205
version: 1
was generated by: iGEM to SBOL conversion
description: XhoI
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:09Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: Restriction Site.
discontinued: false
dominant: true
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Nili Sommovilla
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_I766205_sequence/1

Prefix
type: ComponentDefinition
persistent identity: BBa G00000
display id: BBa_G00000
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick cloning site prefix
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: This part is the standard BioBricks prefix.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: EcoRI site
sequence annotation: Prefix
sequence annotation: NotI site
sequence annotation: XbaI site
sequence: https://synbiohub.org/public/igem/BBa_G00000_sequence/1

EcoRI site
type: SequenceAnnotation
persistent identity: annotation1934223
display id: annotation1934223
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934223/range1934223/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1934223/range1934223/1
type: Range
persistent identity: range1934223
display id: range1934223
version: 1
top level: Prefix
direction: direction/forward
start: 1
end: 6
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation1934225
display id: annotation1934225
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934225/range1934225/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_G00000/annotation1934225/range1934225/1
type: Range
persistent identity: range1934225
display id: range1934225
version: 1
top level: Prefix
direction: direction/forward
start: 1
end: 22
orientation: inline

NotI site
type: SequenceAnnotation
persistent identity: annotation1961228
display id: annotation1961228
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1961228/range1961228/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1961228/range1961228/1
type: Range
persistent identity: range1961228
display id: range1961228
version: 1
top level: Prefix
direction: direction/forward
start: 7
end: 14
orientation: inline

XbaI site
type: SequenceAnnotation
persistent identity: annotation1934224
display id: annotation1934224
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934224/range1934224/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1934224/range1934224/1
type: Range
persistent identity: range1934224
display id: range1934224
version: 1
top level: Prefix
direction: direction/forward
start: 16
end: 21
orientation: inline

CmR
type: ComponentDefinition
persistent identity: BBa K300999
display id: BBa_K300999
version: 1
was generated by: iGEM to SBOL conversion
description: chloramphenicol resistance cassette (rev)
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: <partinfo>BBa_P1004</partinfo>
owned by: james
owned by: myers
mutable description: Reverse complement of <partinfo>BBa_P1004</partinfo> (chloramphenicol resistance cassette without transcriptional terminator).
discontinued: false
dominant: false
experience: experience/Works
group u list: _420_
m user id: 0
owner id: 2621
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: see <partinfo>BBa_P1004</partinfo> for detailed information.
star: false
creator: Lorenzo Pasotti, Manuel Lupotto, Paolo Magni
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: cmR(rev)
sequence: https://synbiohub.org/public/igem/BBa_K300999_sequence/1

cmR(rev)
type: SequenceAnnotation
persistent identity: annotation2081291
display id: annotation2081291
version: 1
top level: CmR
location: https://synbiohub.org/public/igem/BBa_K300999/annotation2081291/range2081291/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K300999/annotation2081291/range2081291/1
type: Range
persistent identity: range2081291
display id: range2081291
version: 1
top level: CmR
direction: direction/reverse
start: 1
end: 663
orientation: inline

NsiI
type: ComponentDefinition
persistent identity: BBa B0046
display id: BBa_B0046
version: 1
was generated by: iGEM to SBOL conversion
description: NsiI restriction enzyme site (PstI compatible overhang)
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: NsiI recognition site. Digestion with NsiI generates a compatible cohesive end to PstI.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: NsiI site
sequence: https://synbiohub.org/public/igem/BBa_B0046_sequence/1

NsiI site
type: SequenceAnnotation
persistent identity: annotation1822730
display id: annotation1822730
version: 1
top level: NsiI
location: https://synbiohub.org/public/igem/BBa_B0046/annotation1822730/range1822730/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0046/annotation1822730/range1822730/1
type: Range
persistent identity: range1822730
display id: range1822730
version: 1
top level: NsiI
direction: direction/forward
start: 1
end: 6
orientation: inline

https://synbiohub.org/public/igem/BBa_J107077_sequence/1
type: Sequence
persistent identity: BBa J107077 sequence
display id: BBa_J107077_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtttacagctagctcagtcctaggtattatgctagctactagagctcatgaactatgaagaggtgacgtcatgaacaaaggtgtaatgcgaccgggccatgtgcagctgcgtgtactggacatgagcaaggccctggaacactacgtcgagttgctgggcctgatcgagatggaccgtgacgaccagggccgtgtctatctgaaggcttggaccgaagtggataagttttccctggtgctacgcgaggctgacgagccgggcatggattttatgggtttcaaggttgtggatgaggatgctctccggcaactggagcgggatctgatggcatatggctgtgccgttgagcagctacccgcaggtgaactgaacagttgtggccggcgcgtgcgcttccaggccccctccgggcatcacttcgagttgtatgcagacaaggaatatactggaaagtggggtttgaatgacgtcaatcccgaggcatggccgcgcgatctgaaaggtatggcggctgtgcgtttcgaccacgccctcatgtatggcgacgaattgccggcgacctatgacctgttcaccaaggtgctcggtttctatctggccgaacaggtgctggacgaaaatggcacgcgcgtcgcccagtttctcagtctgtcgaccaaggcccacgacgtggccttcattcaccatccggaaaaaggccgcctccatcatgtgtccttccacctcgaaacctgggaagacttgcttcgcgccgccgacctgatctccatgaccgacacatctatcgatatcggcccaacccgccacggcctcactcacggcaagaccatctacttcttcgacccgtccggtaaccgcaacgaagtgttctgcgggggagattacaactacccggaccacaaaccggtgacctggaccaccgaccagctgggcaaggcgatcttttaccacgaccgcattctcaacgaacgattcatgaccgtgctgacctgatggtccggagatctccaattattgaaggccgctaacgcggcctttttttgtttctggtctcccaagcttgtatggacagttttccctttgatatgtaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagccataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttatgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgaacgtattggttataagtgaacgataccgtccg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J107113_sequence/1
type: Sequence
persistent identity: BBa J107113 sequence
display id: BBa_J107113_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaattattgaaggccgctaacgcggcctttttttgtttctggtctccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23101_sequence/1
type: Sequence
persistent identity: BBa J23101 sequence
display id: BBa_J23101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J107112_sequence/1
type: Sequence
persistent identity: BBa J107112 sequence
display id: BBa_J107112_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtatggacagttttccctttgatatgtaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagccataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttatgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgaacgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattacatgtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcggttgcggccgcaatcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagcagttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggcagcggtatcatcaacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J107114_sequence/1
type: Sequence
persistent identity: BBa J107114 sequence
display id: BBa_J107114_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggaccgaaaaataaaaagacgcttttcagcgtctttttatttttctttggtaccgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M300055_sequence/1
type: Sequence
persistent identity: BBa M300055 sequence
display id: BBa_M300055_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I766205_sequence/1
type: Sequence
persistent identity: BBa I766205 sequence
display id: BBa_I766205_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00000_sequence/1
type: Sequence
persistent identity: BBa G00000 sequence
display id: BBa_G00000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00001_sequence/1
type: Sequence
persistent identity: BBa G00001 sequence
display id: BBa_G00001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0002_sequence/1
type: Sequence
persistent identity: BBa G0002 sequence
display id: BBa_G0002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J33204_sequence/1
type: Sequence
persistent identity: BBa J33204 sequence
display id: BBa_J33204_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcatgaactatgaagaggtgacgtcatgaacaaaggtgtaatgcgaccgggccatgtgcagctgcgtgtactggacatgagcaaggccctggaacactacgtcgagttgctgggcctgatcgagatggaccgtgacgaccagggccgtgtctatctgaaggcttggaccgaagtggataagttttccctggtgctacgcgaggctgacgagccgggcatggattttatgggtttcaaggttgtggatgaggatgctctccggcaactggagcgggatctgatggcatatggctgtgccgttgagcagctacccgcaggtgaactgaacagttgtggccggcgcgtgcgcttccaggccccctccgggcatcacttcgagttgtatgcagacaaggaatatactggaaagtggggtttgaatgacgtcaatcccgaggcatggccgcgcgatctgaaaggtatggcggctgtgcgtttcgaccacgccctcatgtatggcgacgaattgccggcgacctatgacctgttcaccaaggtgctcggtttctatctggccgaacaggtgctggacgaaaatggcacgcgcgtcgcccagtttctcagtctgtcgaccaaggcccacgacgtggccttcattcaccatccggaaaaaggccgcctccatcatgtgtccttccacctcgaaacctgggaagacttgcttcgcgccgccgacctgatctccatgaccgacacatctatcgatatcggcccaacccgccacggcctcactcacggcaagaccatctacttcttcgacccgtccggtaaccgcaacgaagtgttctgcgggggagattacaactacccggaccacaaaccggtgacctggaccaccgaccagctgggcaaggcgatcttttaccacgaccgcattctcaacgaacgattcatgaccgtgctgacctgatggtccgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31985_sequence/1
type: Sequence
persistent identity: BBa M31985 sequence
display id: BBa_M31985_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agatct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K300999_sequence/1
type: Sequence
persistent identity: BBa K300999 sequence
display id: BBa_K300999_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattacgccccgccctgccactcatcacagtactgttgtaattcattaagcatgcggccgacatggaagccatcacaaacggcatgatgaacctggatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatcgtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacaaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtggagaaactgccggaaatcgtcgtggtattcactccagagggacgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccagatcaccagctcaccgtctttcatggccatacgaaactccgggtgagcgttcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatcccgtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0046_sequence/1
type: Sequence
persistent identity: BBa B0046 sequence
display id: BBa_B0046_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z