LOCUS BBa_J11005 952 bp DNA linear 25-Nov-2024 DEFINITION temperature sensor - low temperature color ACCESSION BBa_J11005 VERSION BBa_J11005.1 FEATURES Location/Qualifiers misc_feature 9..25 /label=OR1 misc_feature 33..49 /label=OR2 promoter 48..53 /label=-35 misc_feature 59..65 /label=mutate to obliterate OR3 promoter 71..76 /label=-10 promoter 1..82 /label=BBa_I12007 RBS 91..103 /label=RBS-3\Weak RBS 97..100 /label=RBS misc_feature 91..103 /label=BBa_B0032 RBS 91..103 /label=BBa_B0032 CDS 110..784 /label=mrfp1 misc_feature 791..815 /label=Help:Barcodes CDS 110..790 /label=BBa_E1010 stem_loop 835..878 /label=stem_loop misc_feature 824..903 /label=BBa_B0010 terminator 824..903 /label=BBa_B0010 stem_loop 919..938 /label=T7 TE misc_feature 945..945 /label=stop polyA_site 939..952 /label=polya misc_feature 912..952 /label=BBa_B0012 terminator 912..952 /label=BBa_B0012 ORIGIN 1 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atatttataa 61 atagtggtga tagatttaac gttactagag tcacacagga aagtactaga tggcttcctc 121 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg 181 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc 241 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca 301 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa 361 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt 421 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact 481 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga 541 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg 601 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa 661 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca 721 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg 781 tgcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa 841 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 901 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //