LOCUS       BBa_J11007              1893 bp    DNA     linear       01-Jun-2024
DEFINITION  completed 2 color temperature sensor
ACCESSION   BBa_J11007
VERSION     BBa_J11007.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     RBS             58..70
                     /label=RBS-3\Weak
     RBS             64..67
                     /label=RBS
     misc_feature    58..70
                     /label=BBa_B0032
     RBS             58..70
                     /label=BBa_B0032
     CDS             77..796
                     /label=GFP protein
     CDS             77..796
                     /label=BBa_E0040
     stem_loop       816..859
                     /label=stem_loop
     misc_feature    805..884
                     /label=BBa_B0010
     terminator      805..884
                     /label=BBa_B0010
     stem_loop       900..919
                     /label=T7 TE
     misc_feature    926..926
                     /label=stop
     polyA_site      920..933
                     /label=polya
     misc_feature    893..933
                     /label=BBa_B0012
     terminator      893..933
                     /label=BBa_B0012
     misc_feature    1..933
                     /label=BBa_J11004
     misc_feature    950..966
                     /label=OR1
     misc_feature    974..990
                     /label=OR2
     promoter        989..994
                     /label=-35
     misc_feature    1000..1006
                     /label=mutate to obliterate OR3
     promoter        1012..1017
                     /label=-10
     promoter        942..1023
                     /label=BBa_I12007
     RBS             1032..1044
                     /label=RBS-3\Weak
     RBS             1038..1041
                     /label=RBS
     misc_feature    1032..1044
                     /label=BBa_B0032
     RBS             1032..1044
                     /label=BBa_B0032
     CDS             1051..1725
                     /label=mrfp1
     misc_feature    1732..1756
                     /label=Help:Barcodes
     CDS             1051..1731
                     /label=BBa_E1010
     stem_loop       1776..1819
                     /label=stem_loop
     misc_feature    1765..1844
                     /label=BBa_B0010
     terminator      1765..1844
                     /label=BBa_B0010
     stem_loop       1860..1879
                     /label=T7 TE
     misc_feature    1886..1886
                     /label=stop
     polyA_site      1880..1893
                     /label=polya
     misc_feature    1853..1893
                     /label=BBa_B0012
     terminator      1853..1893
                     /label=BBa_B0012
     misc_feature    942..1893
                     /label=BBa_J11005
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca
       61 cacaggaaag tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct
      121 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
      181 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
      241 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc
      301 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
      361 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
      421 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
      481 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
      541 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
      601 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
      661 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
      721 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
      781 actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
      841 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      901 gctcaccttc gggtgggcct ttctgcgttt atatactaga ggcaaccatt atcaccgcca
      961 gaggtaaaat agtcaacacg cacggtgtta gatatttata aatagtggtg atagatttaa
     1021 cgttactaga gtcacacagg aaagtactag atggcttcct ccgaagacgt tatcaaagag
     1081 ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa
     1141 ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa
     1201 ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa
     1261 gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt
     1321 ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac
     1381 tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg
     1441 tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg
     1501 tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt
     1561 ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg
     1621 ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc
     1681 gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag
     1741 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1801 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1861 gctcaccttc gggtgggcct ttctgcgttt ata
//