LOCUS       BBa_J11008               934 bp    DNA     linear       16-Feb-2025
DEFINITION  variant of J11004 (hi temp color) with weaker RBS
ACCESSION   BBa_J11008
VERSION     BBa_J11008.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    64..67
                     /label=conserved
     RBS             58..71
                     /label=BBa_B0031
     CDS             78..797
                     /label=GFP protein
     CDS             78..797
                     /label=BBa_E0040
     stem_loop       817..860
                     /label=stem_loop
     misc_feature    806..885
                     /label=BBa_B0010
     terminator      806..885
                     /label=BBa_B0010
     stem_loop       901..920
                     /label=T7 TE
     misc_feature    927..927
                     /label=stop
     polyA_site      921..934
                     /label=polya
     misc_feature    894..934
                     /label=BBa_B0012
     terminator      894..934
                     /label=BBa_B0012
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca
       61 cacaggaaac ctactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc
      121 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
      181 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
      241 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc
      301 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
      361 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
      421 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
      481 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
      541 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
      601 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
      661 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
      721 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
      781 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
      841 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
      901 ggctcacctt cgggtgggcc tttctgcgtt tata
//