LOCUS       BBa_J11010              1896 bp    DNA     linear       22-Dec-2024
DEFINITION  completed 2 color temperature sensor with rfp (low) bias
ACCESSION   BBa_J11010
VERSION     BBa_J11010.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    64..67
                     /label=conserved
     RBS             58..71
                     /label=BBa_B0031
     CDS             78..797
                     /label=GFP protein
     CDS             78..797
                     /label=BBa_E0040
     stem_loop       817..860
                     /label=stem_loop
     misc_feature    806..885
                     /label=BBa_B0010
     terminator      806..885
                     /label=BBa_B0010
     stem_loop       901..920
                     /label=T7 TE
     misc_feature    927..927
                     /label=stop
     polyA_site      921..934
                     /label=polya
     misc_feature    894..934
                     /label=BBa_B0012
     terminator      894..934
                     /label=BBa_B0012
     misc_feature    1..934
                     /label=BBa_J11008
     misc_feature    951..967
                     /label=OR1
     misc_feature    975..991
                     /label=OR2
     promoter        990..995
                     /label=-35
     misc_feature    1001..1007
                     /label=mutate to obliterate OR3
     promoter        1013..1018
                     /label=-10
     promoter        943..1024
                     /label=BBa_I12007
     RBS             1033..1047
                     /label=RBS-1\Strong
     RBS             1040..1044
                     /label=RBS
     misc_feature    1033..1047
                     /label=BBa_B0030
     RBS             1033..1047
                     /label=BBa_B0030
     CDS             1054..1728
                     /label=mrfp1
     misc_feature    1735..1759
                     /label=Help:Barcodes
     CDS             1054..1734
                     /label=BBa_E1010
     stem_loop       1779..1822
                     /label=stem_loop
     misc_feature    1768..1847
                     /label=BBa_B0010
     terminator      1768..1847
                     /label=BBa_B0010
     stem_loop       1863..1882
                     /label=T7 TE
     misc_feature    1889..1889
                     /label=stop
     polyA_site      1883..1896
                     /label=polya
     misc_feature    1856..1896
                     /label=BBa_B0012
     terminator      1856..1896
                     /label=BBa_B0012
     misc_feature    943..1896
                     /label=BBa_J11009
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca
       61 cacaggaaac ctactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc
      121 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
      181 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
      241 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc
      301 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
      361 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
      421 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
      481 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
      541 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
      601 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
      661 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
      721 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
      781 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
      841 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
      901 ggctcacctt cgggtgggcc tttctgcgtt tatatactag aggcaaccat tatcaccgcc
      961 agaggtaaaa tagtcaacac gcacggtgtt agatatttat aaatagtggt gatagattta
     1021 acgttactag agattaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa
     1081 gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc
     1141 gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc
     1201 aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc
     1261 aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa
     1321 ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag
     1381 gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc
     1441 ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt
     1501 atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac
     1561 ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg
     1621 ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc
     1681 atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga
     1741 tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1801 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1861 ctggctcacc ttcgggtggg cctttctgcg tttata
//