LOCUS       BBa_J11012               898 bp    DNA     linear       15-May-2024
DEFINITION  constitutive production of tetR
ACCESSION   BBa_J11012
VERSION     BBa_J11012.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     RBS             58..70
                     /label=RBS-3\Weak
     RBS             64..67
                     /label=RBS
     misc_feature    58..70
                     /label=BBa_B0032
     RBS             58..70
                     /label=BBa_B0032
     CDS             80..696
                     /label=tetR
     misc_feature    697..730
                     /label=SsrA
     misc_feature    737..761
                     /label=Help:Barcodes
     CDS             77..736
                     /label=BBa_C0040
     stem_loop       781..824
                     /label=stem_loop
     misc_feature    770..849
                     /label=BBa_B0010
     terminator      770..849
                     /label=BBa_B0010
     stem_loop       865..884
                     /label=T7 TE
     misc_feature    891..891
                     /label=stop
     polyA_site      885..898
                     /label=polya
     misc_feature    858..898
                     /label=BBa_B0012
     terminator      858..898
                     /label=BBa_B0012
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca
       61 cacaggaaag tactagatgt ccagattaga taaaagtaaa gtgattaaca gcgcattaga
      121 gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg
      181 tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt
      241 agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg
      301 gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga
      361 tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa
      421 tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag
      481 cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa
      541 agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga
      601 attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg
      661 cggattagaa aaacaactta aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc
      721 tttagtagct taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa
      781 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      841 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//