LOCUS       BBa_J11013               995 bp    DNA     linear       14-May-2024
DEFINITION  tet regulated control of CI(ts)
ACCESSION   BBa_J11013
VERSION     BBa_J11013.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     CDS             84..794
                     /label=cI857
     misc_feature    795..827
                     /label=LVA
     misc_feature    282..282
                     /label=G->A (857)
     misc_feature    435..435
                     /label=G->A (ind-)
     misc_feature    834..858
                     /label=barcode from LacI-LVA (BBa_C0012)
     CDS             84..858
                     /label=BBa_J06503
     stem_loop       878..921
                     /label=stem_loop
     misc_feature    867..946
                     /label=BBa_B0010
     terminator      867..946
                     /label=BBa_B0010
     stem_loop       962..981
                     /label=T7 TE
     misc_feature    988..988
                     /label=stop
     polyA_site      982..995
                     /label=polya
     misc_feature    955..995
                     /label=BBa_B0012
     terminator      955..995
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatgagca caaaaaagaa accattaaca caagagcagc
      121 ttgaggacgc acgtcgcctt aaagcaattt atgaaaaaaa gaaaaatgaa cttggcttat
      181 cccaggaatc tgtcgcagac aagatgggga tggggcagtc aggcgttggt gctttattta
      241 atggcatcaa tgcattaaat gcttataacg ccgcattgct tacaaaaatt ctcaaagtta
      301 gcgttgaaga atttagccct tcaatcgcca gagaaatcta cgagatgtat gaagcggtta
      361 gtatgcagcc gtcacttaga agtgagtatg agtaccctgt tttttctcat gttcaggcag
      421 ggatgttctc acctaagctt agaaccttta ccaaaggtga tgcggagaga tgggtaagca
      481 caaccaaaaa agccagtgat tctgcattct ggcttgaggt tgaaggtaat tccatgaccg
      541 caccaacagg ctccaagcca agctttcctg acggaatgtt aattctcgtt gaccctgagc
      601 aggctgttga gccaggtgat ttctgcatag ccagacttgg gggtgatgag tttaccttca
      661 agaaactgat cagggatagc ggtcaggtgt ttttacaacc actaaaccca cagtacccaa
      721 tgatcccatg caatgagagt tgttccgttg tggggaaagt tatcgctagt cagtggcctg
      781 aagagacgtt tggcgctgca aacgacgaaa actacgcttt agtagcttaa taacgctgat
      841 agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      901 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      961 tggctcacct tcgggtgggc ctttctgcgt ttata
//