LOCUS BBa_J11014 3805 bp DNA linear 26-Dec-2024 DEFINITION completed 2 color temperature sensor, tetracycline tuneable ACCESSION BBa_J11014 VERSION BBa_J11014.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 misc_feature 64..67 /label=conserved RBS 58..71 /label=BBa_B0031 CDS 78..797 /label=GFP protein CDS 78..797 /label=BBa_E0040 stem_loop 817..860 /label=stem_loop misc_feature 806..885 /label=BBa_B0010 terminator 806..885 /label=BBa_B0010 stem_loop 901..920 /label=T7 TE misc_feature 927..927 /label=stop polyA_site 921..934 /label=polya misc_feature 894..934 /label=BBa_B0012 terminator 894..934 /label=BBa_B0012 misc_feature 1..934 /label=BBa_J11008 misc_feature 951..967 /label=OR1 misc_feature 975..991 /label=OR2 promoter 990..995 /label=-35 misc_feature 1001..1007 /label=mutate to obliterate OR3 promoter 1013..1018 /label=-10 promoter 943..1024 /label=BBa_I12007 RBS 1033..1047 /label=RBS-1\Strong RBS 1040..1044 /label=RBS misc_feature 1033..1047 /label=BBa_B0030 RBS 1033..1047 /label=BBa_B0030 CDS 1054..1728 /label=mrfp1 misc_feature 1735..1759 /label=Help:Barcodes CDS 1054..1734 /label=BBa_E1010 stem_loop 1779..1822 /label=stem_loop misc_feature 1768..1847 /label=BBa_B0010 terminator 1768..1847 /label=BBa_B0010 stem_loop 1863..1882 /label=T7 TE misc_feature 1889..1889 /label=stop polyA_site 1883..1896 /label=polya misc_feature 1856..1896 /label=BBa_B0012 terminator 1856..1896 /label=BBa_B0012 misc_feature 943..1896 /label=BBa_J11011 promoter 1942..1947 /label=-10 promoter 1919..1924 /label=-35 misc_feature 1929..1945 /label=OR1 misc_feature 1905..1921 /label=OR2 misc_feature 1905..1953 /label=BBa_R0051 promoter 1905..1953 /label=BBa_R0051 RBS 1962..1974 /label=RBS-3\Weak RBS 1968..1971 /label=RBS misc_feature 1962..1974 /label=BBa_B0032 RBS 1962..1974 /label=BBa_B0032 CDS 1984..2600 /label=tetR misc_feature 2601..2634 /label=SsrA misc_feature 2641..2665 /label=Help:Barcodes CDS 1981..2640 /label=BBa_C0040 stem_loop 2685..2728 /label=stem_loop misc_feature 2674..2753 /label=BBa_B0010 terminator 2674..2753 /label=BBa_B0010 stem_loop 2769..2788 /label=T7 TE misc_feature 2795..2795 /label=stop polyA_site 2789..2802 /label=polya misc_feature 2762..2802 /label=BBa_B0012 terminator 2762..2802 /label=BBa_B0012 misc_feature 1905..2802 /label=BBa_J11012 misc_feature 2811..2829 /label=TetR 1 misc_feature 2811..2864 /label=BBa_R0040 promoter 2830..2835 /label=-35 misc_feature 2836..2854 /label=TetR 2 promoter 2853..2858 /label=-10 promoter 2811..2864 /label=BBa_R0040 RBS 2873..2887 /label=RBS-1\Strong RBS 2880..2884 /label=RBS misc_feature 2873..2887 /label=BBa_B0030 RBS 2873..2887 /label=BBa_B0030 CDS 2894..3604 /label=cI857 misc_feature 3605..3637 /label=LVA misc_feature 3092..3092 /label=G->A (857) misc_feature 3245..3245 /label=G->A (ind-) misc_feature 3644..3668 /label=barcode from LacI-LVA (BBa_C0012) CDS 2894..3668 /label=BBa_J06503 stem_loop 3688..3731 /label=stem_loop misc_feature 3677..3756 /label=BBa_B0010 terminator 3677..3756 /label=BBa_B0010 stem_loop 3772..3791 /label=T7 TE misc_feature 3798..3798 /label=stop polyA_site 3792..3805 /label=polya misc_feature 3765..3805 /label=BBa_B0012 terminator 3765..3805 /label=BBa_B0012 misc_feature 2811..3805 /label=BBa_J11013 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca 61 cacaggaaac ctactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc 121 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag 181 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg 241 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc 301 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg 361 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg 421 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa 481 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag 541 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa 601 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt 661 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga 721 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg 781 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 841 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 901 ggctcacctt cgggtgggcc tttctgcgtt tatatactag aggcaaccat tatcaccgcc 961 agaggtaaaa tagtcaacac gcacggtgtt agatatttat aaatagtggt gatagattta 1021 acgttactag agattaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa 1081 gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc 1141 gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc 1201 aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc 1261 aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa 1321 ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag 1381 gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc 1441 ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt 1501 atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac 1561 ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg 1621 ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc 1681 atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga 1741 tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga 1801 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 1861 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtaacac cgtgcgtgtt 1921 gactatttta cctctggcgg tgataatggt tgctactaga gtcacacagg aaagtactag 1981 atgtccagat tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc 2041 ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca 2101 ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta 2161 gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt 2221 aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat 2281 ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta 2341 tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt 2401 actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca 2461 cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa 2521 ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa 2581 cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact acgctttagt agcttaataa 2641 cactgatagt gctagtgtag atcactacta gagccaggca tcaaataaaa cgaaaggctc 2701 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 2761 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag tccctatcag 2821 tgatagagat tgacatccct atcagtgata gagatactga gcactactag agattaaaga 2881 ggagaaatac tagatgagca caaaaaagaa accattaaca caagagcagc ttgaggacgc 2941 acgtcgcctt aaagcaattt atgaaaaaaa gaaaaatgaa cttggcttat cccaggaatc 3001 tgtcgcagac aagatgggga tggggcagtc aggcgttggt gctttattta atggcatcaa 3061 tgcattaaat gcttataacg ccgcattgct tacaaaaatt ctcaaagtta gcgttgaaga 3121 atttagccct tcaatcgcca gagaaatcta cgagatgtat gaagcggtta gtatgcagcc 3181 gtcacttaga agtgagtatg agtaccctgt tttttctcat gttcaggcag ggatgttctc 3241 acctaagctt agaaccttta ccaaaggtga tgcggagaga tgggtaagca caaccaaaaa 3301 agccagtgat tctgcattct ggcttgaggt tgaaggtaat tccatgaccg caccaacagg 3361 ctccaagcca agctttcctg acggaatgtt aattctcgtt gaccctgagc aggctgttga 3421 gccaggtgat ttctgcatag ccagacttgg gggtgatgag tttaccttca agaaactgat 3481 cagggatagc ggtcaggtgt ttttacaacc actaaaccca cagtacccaa tgatcccatg 3541 caatgagagt tgttccgttg tggggaaagt tatcgctagt cagtggcctg aagagacgtt 3601 tggcgctgca aacgacgaaa actacgcttt agtagcttaa taacgctgat agtgctagtg 3661 tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 3721 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 3781 tcgggtgggc ctttctgcgt ttata //