##gff-version 3
##sequence-region BBa_J11014 1 3805
BBa_J11014	.	sequence_feature	1905	2802	.	+	0	ID=BBa_J11012;Name=BBa_J11012
BBa_J11014	.	ribosome_entry_site	1962	1974	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J11012
BBa_J11014	.	engineered_region	1962	1974	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J11014	.	ribosome_entry_site	1968	1971	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J11014	.	ribosome_entry_site	1962	1974	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J11014	.	promoter	1905	1953	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_J11012
BBa_J11014	.	engineered_region	1905	1953	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J11014	.	non_covalent_binding_site	1929	1945	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J11014	.	non_covalent_binding_site	1905	1921	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_J11014	.	promoter	1942	1947	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J11014	.	promoter	1919	1924	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J11014	.	terminator	2674	2753	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J11012
BBa_J11014	.	engineered_region	2674	2753	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J11014	.	stem_loop	2685	2728	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J11014	.	terminator	2762	2802	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J11012
BBa_J11014	.	stem_loop	2769	2788	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J11014	.	stop_codon	2795	2795	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J11014	.	polyA_site	2789	2802	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J11014	.	engineered_region	2762	2802	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J11014	.	CDS	1981	2640	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_J11012
BBa_J11014	.	engineered_tag	2641	2665	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J11014	.	CDS	1984	2600	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J11014	.	tag	2601	2634	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J11014	.	sequence_feature	943	1896	.	+	0	ID=BBa_J11011;Name=BBa_J11011
BBa_J11014	.	ribosome_entry_site	1033	1047	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J11011
BBa_J11014	.	engineered_region	1033	1047	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J11014	.	ribosome_entry_site	1040	1044	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J11014	.	ribosome_entry_site	1033	1047	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J11014	.	CDS	1054	1734	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J11011
BBa_J11014	.	CDS	1054	1728	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J11014	.	engineered_tag	1735	1759	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J11014	.	promoter	943	1024	.	+	0	ID=BBa_I12007;Name=BBa_I12007;Parent=BBa_J11011
BBa_J11014	.	promoter	1013	1018	.	+	0	ID=annotation937705;Name=-10;Parent=BBa_I12007
BBa_J11014	.	sequence_alteration	1001	1007	.	+	0	ID=annotation937704;Name=mutate to obliterate OR3;Parent=BBa_I12007
BBa_J11014	.	non_covalent_binding_site	951	967	.	+	0	ID=annotation937701;Name=OR1;Parent=BBa_I12007
BBa_J11014	.	non_covalent_binding_site	975	991	.	+	0	ID=annotation937702;Name=OR2;Parent=BBa_I12007
BBa_J11014	.	promoter	990	995	.	+	0	ID=annotation937703;Name=-35;Parent=BBa_I12007
BBa_J11014	.	terminator	1856	1896	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J11011
BBa_J11014	.	stem_loop	1863	1882	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J11014	.	stop_codon	1889	1889	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J11014	.	polyA_site	1883	1896	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J11014	.	engineered_region	1856	1896	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J11014	.	terminator	1768	1847	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J11011
BBa_J11014	.	engineered_region	1768	1847	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J11014	.	stem_loop	1779	1822	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J11014	.	sequence_feature	2811	3805	.	+	0	ID=BBa_J11013;Name=BBa_J11013
BBa_J11014	.	CDS	2894	3668	.	+	0	ID=BBa_J06503;Name=BBa_J06503;Parent=BBa_J11013
BBa_J11014	.	sequence_alteration	3245	3245	.	+	0	ID=annotation1549981;Name=G->A (ind-);Parent=BBa_J06503
BBa_J11014	.	sequence_alteration	3092	3092	.	+	0	ID=annotation1549980;Name=G->A (857);Parent=BBa_J06503
BBa_J11014	.	tag	3605	3637	.	+	0	ID=annotation1549979;Name=LVA;Parent=BBa_J06503
BBa_J11014	.	engineered_tag	3644	3668	.	+	0	ID=annotation1552049;Name=barcode from LacI-LVA (BBa_C0012);Parent=BBa_J06503
BBa_J11014	.	CDS	2894	3604	.	+	0	ID=annotation1549975;Name=cI857;Parent=BBa_J06503
BBa_J11014	.	promoter	2811	2864	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J11013
BBa_J11014	.	engineered_region	2811	2864	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J11014	.	promoter	2830	2835	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J11014	.	non_covalent_binding_site	2836	2854	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J11014	.	promoter	2853	2858	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J11014	.	non_covalent_binding_site	2811	2829	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J11014	.	terminator	3677	3756	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J11013
BBa_J11014	.	engineered_region	3677	3756	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J11014	.	stem_loop	3688	3731	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J11014	.	ribosome_entry_site	2873	2887	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J11013
BBa_J11014	.	engineered_region	2873	2887	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J11014	.	ribosome_entry_site	2880	2884	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J11014	.	ribosome_entry_site	2873	2887	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J11014	.	terminator	3765	3805	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J11013
BBa_J11014	.	stem_loop	3772	3791	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J11014	.	stop_codon	3798	3798	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J11014	.	polyA_site	3792	3805	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J11014	.	engineered_region	3765	3805	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J11014	.	sequence_feature	1	934	.	+	0	ID=BBa_J11008;Name=BBa_J11008
BBa_J11014	.	terminator	806	885	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J11008
BBa_J11014	.	engineered_region	806	885	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J11014	.	stem_loop	817	860	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J11014	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_J11008
BBa_J11014	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J11014	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J11014	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_J11014	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J11014	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J11014	.	CDS	78	797	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_J11008
BBa_J11014	.	CDS	78	797	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J11014	.	terminator	894	934	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J11008
BBa_J11014	.	stem_loop	901	920	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J11014	.	stop_codon	927	927	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J11014	.	polyA_site	921	934	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J11014	.	engineered_region	894	934	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J11014	.	ribosome_entry_site	58	71	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J11008
BBa_J11014	.	conserved_region	64	67	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
>BBa_J11014
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaacctactagat
gcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattt
tctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactac
ctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaaca
gcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactac
aagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaag
atggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaa
tggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaat
actccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatccca
acgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaa
ataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaacc
attatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttac
tagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtat
ggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgct
aaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaag
cttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttat
gaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaa
ctgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgta
tgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctga
agttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacc
tcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgct
gatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
tactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaagt
actagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggttt
aacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttg
ctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatt
ttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacg
gcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgca
ttatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaag
aaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcaga
gccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgca
aacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatca
gtgatagagatactgagcactactagagattaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaa
gagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctg
tcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgc
cgcattgcttacaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtat
gaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttct
cacctaagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcatt
ctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctc
gttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaac
tgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttc
cgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagta
gcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttata