BBa_J11014
type: ComponentDefinition
persistent identity: BBa J11014
display id: BBa_J11014
version: 1
was generated by: iGEM to SBOL conversion
description: completed 2 color temperature sensor, tetracycline tuneable
created: 2005-07-26T11:00:00Z
modified: 2015-08-31T04:08:25Z
owned by: james
owned by: myers
mutable description: 2 color temperature sensor, with regulatory system decoupled from color production and under the control of a tetracycline repressor for tuning
discontinued: false
dominant: false
group u list: _38_
m user id: 0
owner id: 341
owning group id: 38
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Andrew Hessel
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J11008
component: BBa_J11013
component: BBa_J11012
component: BBa_J11011
sequence annotation: BBa_J11008
sequence annotation: BBa_J11011
sequence annotation: BBa_J11012
sequence annotation: BBa_J11013
sequence: https://synbiohub.org/public/igem/BBa_J11014_sequence/1

BBa_J11008
type: Component
persistent identity: component2238113
display id: component2238113
version: 1
top level: BBa_J11014
definition: BBa_J11008
access: public

BBa_J11013
type: Component
persistent identity: component2238172
display id: component2238172
version: 1
top level: BBa_J11014
definition: BBa_J11013
access: public

BBa_J11012
type: Component
persistent identity: component2238151
display id: component2238151
version: 1
top level: BBa_J11014
definition: BBa_J11012
access: public

BBa_J11011
type: Component
persistent identity: component2238132
display id: component2238132
version: 1
top level: BBa_J11014
definition: BBa_J11011
access: public

BBa_J11008
type: SequenceAnnotation
persistent identity: annotation2238113
display id: annotation2238113
version: 1
top level: BBa_J11014
location: https://synbiohub.org/public/igem/BBa_J11014/annotation2238113/range2238113/1
component: BBa_J11008

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type: Range
persistent identity: range2238113
display id: range2238113
version: 1
top level: BBa_J11014
direction: direction/reverse
start: 1
end: 934
orientation: inline

BBa_J11011
type: SequenceAnnotation
persistent identity: annotation2238132
display id: annotation2238132
version: 1
top level: BBa_J11014
location: https://synbiohub.org/public/igem/BBa_J11014/annotation2238132/range2238132/1
component: BBa_J11011

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type: Range
persistent identity: range2238132
display id: range2238132
version: 1
top level: BBa_J11014
direction: direction/reverse
start: 943
end: 1896
orientation: inline

BBa_J11012
type: SequenceAnnotation
persistent identity: annotation2238151
display id: annotation2238151
version: 1
top level: BBa_J11014
location: https://synbiohub.org/public/igem/BBa_J11014/annotation2238151/range2238151/1
component: BBa_J11012

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persistent identity: range2238151
display id: range2238151
version: 1
top level: BBa_J11014
direction: direction/reverse
start: 1905
end: 2802
orientation: inline

BBa_J11013
type: SequenceAnnotation
persistent identity: annotation2238172
display id: annotation2238172
version: 1
top level: BBa_J11014
location: https://synbiohub.org/public/igem/BBa_J11014/annotation2238172/range2238172/1
component: BBa_J11013

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persistent identity: range2238172
display id: range2238172
version: 1
top level: BBa_J11014
direction: direction/reverse
start: 2811
end: 3805
orientation: inline

BBa_J11008
type: ComponentDefinition
persistent identity: BBa J11008
display id: BBa_J11008
version: 1
was generated by: iGEM to SBOL conversion
description: variant of J11004 (hi temp color) with weaker RBS
created: 2005-07-26T11:00:00Z
modified: 2015-08-31T04:08:25Z
owned by: james
owned by: myers
mutable description: Variant of BBa_J1104 with weaker RBS. Concern is that GFP signal will be too strong. The expression of GFP should not start until 37C, but a weaker promoter will help ensure this.
discontinued: false
dominant: false
group u list: _38_
m user id: 0
owner id: 341
owning group id: 38
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Andrew Hessel
type: DnaRegion
role: partType/Temporary
role: SO:0000110
component: BBa_B0012
component: BBa_B0010
component: BBa_E0040
component: BBa_B0031
component: BBa_R0051
sequence annotation: BBa_R0051
sequence annotation: BBa_B0031
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J11008_sequence/1

BBa_B0012
type: Component
persistent identity: component1595141
display id: component1595141
version: 1
top level: BBa_J11008
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1595131
display id: component1595131
version: 1
top level: BBa_J11008
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1595126
display id: component1595126
version: 1
top level: BBa_J11008
definition: GFP
access: public

BBa_B0031
type: Component
persistent identity: component1595124
display id: component1595124
version: 1
top level: BBa_J11008
definition: BBa_B0031
access: public

BBa_R0051
type: Component
persistent identity: component1595116
display id: component1595116
version: 1
top level: BBa_J11008
definition: cI lam
access: public

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1595116
display id: annotation1595116
version: 1
top level: BBa_J11008
location: https://synbiohub.org/public/igem/BBa_J11008/annotation1595116/range1595116/1
component: BBa_R0051

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type: Range
persistent identity: range1595116
display id: range1595116
version: 1
top level: BBa_J11008
direction: direction/forward
start: 1
end: 49
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation1595124
display id: annotation1595124
version: 1
top level: BBa_J11008
location: https://synbiohub.org/public/igem/BBa_J11008/annotation1595124/range1595124/1
component: BBa_B0031

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type: Range
persistent identity: range1595124
display id: range1595124
version: 1
top level: BBa_J11008
direction: direction/forward
start: 58
end: 71
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1595126
display id: annotation1595126
version: 1
top level: BBa_J11008
location: https://synbiohub.org/public/igem/BBa_J11008/annotation1595126/range1595126/1
component: BBa_E0040

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type: Range
persistent identity: range1595126
display id: range1595126
version: 1
top level: BBa_J11008
direction: direction/forward
start: 78
end: 797
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1595131
display id: annotation1595131
version: 1
top level: BBa_J11008
location: https://synbiohub.org/public/igem/BBa_J11008/annotation1595131/range1595131/1
component: BBa_B0010

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type: Range
persistent identity: range1595131
display id: range1595131
version: 1
top level: BBa_J11008
direction: direction/forward
start: 806
end: 885
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1595141
display id: annotation1595141
version: 1
top level: BBa_J11008
location: https://synbiohub.org/public/igem/BBa_J11008/annotation1595141/range1595141/1
component: BBa_B0012

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type: Range
persistent identity: range1595141
display id: range1595141
version: 1
top level: BBa_J11008
direction: direction/forward
start: 894
end: 934
orientation: inline

BBa_J11011
type: ComponentDefinition
persistent identity: BBa J11011
display id: BBa_J11011
version: 1
was generated by: iGEM to SBOL conversion
description: variant of J11005 uncoupled from CI(ts) production
created: 2005-07-26T11:00:00Z
modified: 2015-08-31T04:08:25Z
owned by: james
owned by: myers
mutable description: low temperature RFP expression uncoupled from CI(ts) protein
discontinued: false
dominant: true
group u list: _38_
m user id: 0
owner id: 341
owning group id: 38
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Andrew Hessel
type: DnaRegion
role: SO:0000110
role: partType/Temporary
component: BBa_B0010
component: BBa_E1010
component: BBa_B0030
component: BBa_I12007
component: BBa_B0012
sequence annotation: BBa_I12007
sequence annotation: BBa_B0030
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J11011_sequence/1

BBa_B0010
type: Component
persistent identity: component1595679
display id: component1595679
version: 1
top level: BBa_J11011
definition: BBa_B0010
access: public

BBa_E1010
type: Component
persistent identity: component1595673
display id: component1595673
version: 1
top level: BBa_J11011
definition: mRFP1
access: public

BBa_B0030
type: Component
persistent identity: component1595665
display id: component1595665
version: 1
top level: BBa_J11011
definition: BBa_B0030
access: public

BBa_I12007
type: Component
persistent identity: component1595657
display id: component1595657
version: 1
top level: BBa_J11011
definition: Prm +
access: public

BBa_B0012
type: Component
persistent identity: component1595689
display id: component1595689
version: 1
top level: BBa_J11011
definition: BBa_B0012
access: public

BBa_I12007
type: SequenceAnnotation
persistent identity: annotation1595657
display id: annotation1595657
version: 1
top level: BBa_J11011
location: https://synbiohub.org/public/igem/BBa_J11011/annotation1595657/range1595657/1
component: BBa_I12007

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type: Range
persistent identity: range1595657
display id: range1595657
version: 1
top level: BBa_J11011
direction: direction/forward
start: 1
end: 82
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1595665
display id: annotation1595665
version: 1
top level: BBa_J11011
location: https://synbiohub.org/public/igem/BBa_J11011/annotation1595665/range1595665/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J11011/annotation1595665/range1595665/1
type: Range
persistent identity: range1595665
display id: range1595665
version: 1
top level: BBa_J11011
direction: direction/forward
start: 91
end: 105
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1595673
display id: annotation1595673
version: 1
top level: BBa_J11011
location: https://synbiohub.org/public/igem/BBa_J11011/annotation1595673/range1595673/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J11011/annotation1595673/range1595673/1
type: Range
persistent identity: range1595673
display id: range1595673
version: 1
top level: BBa_J11011
direction: direction/forward
start: 112
end: 792
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1595679
display id: annotation1595679
version: 1
top level: BBa_J11011
location: https://synbiohub.org/public/igem/BBa_J11011/annotation1595679/range1595679/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J11011/annotation1595679/range1595679/1
type: Range
persistent identity: range1595679
display id: range1595679
version: 1
top level: BBa_J11011
direction: direction/forward
start: 826
end: 905
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1595689
display id: annotation1595689
version: 1
top level: BBa_J11011
location: https://synbiohub.org/public/igem/BBa_J11011/annotation1595689/range1595689/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J11011/annotation1595689/range1595689/1
type: Range
persistent identity: range1595689
display id: range1595689
version: 1
top level: BBa_J11011
direction: direction/forward
start: 914
end: 954
orientation: inline

BBa_J11012
type: ComponentDefinition
persistent identity: BBa J11012
display id: BBa_J11012
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive production of tetR
created: 2005-07-26T11:00:00Z
modified: 2015-08-31T04:08:25Z
owned by: james
owned by: myers
mutable description: constitutive expressioin of tetR protein
discontinued: false
dominant: false
group u list: _38_
m user id: 0
owner id: 341
owning group id: 38
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Andrew Hessel
type: DnaRegion
role: partType/Temporary
role: SO:0000110
component: BBa_R0051
component: BBa_B0012
component: BBa_B0010
component: BBa_C0040
component: BBa_B0032
sequence annotation: BBa_R0051
sequence annotation: BBa_B0032
sequence annotation: BBa_C0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J11012_sequence/1

BBa_R0051
type: Component
persistent identity: component1595939
display id: component1595939
version: 1
top level: BBa_J11012
definition: cI lam
access: public

BBa_B0012
type: Component
persistent identity: component1595977
display id: component1595977
version: 1
top level: BBa_J11012
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1595967
display id: component1595967
version: 1
top level: BBa_J11012
definition: BBa_B0010
access: public

BBa_C0040
type: Component
persistent identity: component1595961
display id: component1595961
version: 1
top level: BBa_J11012
definition: tetR
access: public

BBa_B0032
type: Component
persistent identity: component1595950
display id: component1595950
version: 1
top level: BBa_J11012
definition: BBa_B0032
access: public

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1595939
display id: annotation1595939
version: 1
top level: BBa_J11012
location: https://synbiohub.org/public/igem/BBa_J11012/annotation1595939/range1595939/1
component: BBa_R0051

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type: Range
persistent identity: range1595939
display id: range1595939
version: 1
top level: BBa_J11012
direction: direction/forward
start: 1
end: 49
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1595950
display id: annotation1595950
version: 1
top level: BBa_J11012
location: https://synbiohub.org/public/igem/BBa_J11012/annotation1595950/range1595950/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J11012/annotation1595950/range1595950/1
type: Range
persistent identity: range1595950
display id: range1595950
version: 1
top level: BBa_J11012
direction: direction/forward
start: 58
end: 70
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation1595961
display id: annotation1595961
version: 1
top level: BBa_J11012
location: https://synbiohub.org/public/igem/BBa_J11012/annotation1595961/range1595961/1
component: BBa_C0040

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type: Range
persistent identity: range1595961
display id: range1595961
version: 1
top level: BBa_J11012
direction: direction/forward
start: 77
end: 736
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1595967
display id: annotation1595967
version: 1
top level: BBa_J11012
location: https://synbiohub.org/public/igem/BBa_J11012/annotation1595967/range1595967/1
component: BBa_B0010

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type: Range
persistent identity: range1595967
display id: range1595967
version: 1
top level: BBa_J11012
direction: direction/forward
start: 770
end: 849
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1595977
display id: annotation1595977
version: 1
top level: BBa_J11012
location: https://synbiohub.org/public/igem/BBa_J11012/annotation1595977/range1595977/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J11012/annotation1595977/range1595977/1
type: Range
persistent identity: range1595977
display id: range1595977
version: 1
top level: BBa_J11012
direction: direction/forward
start: 858
end: 898
orientation: inline

BBa_J11013
type: ComponentDefinition
persistent identity: BBa J11013
display id: BBa_J11013
version: 1
was generated by: iGEM to SBOL conversion
description: tet regulated control of CI(ts)
created: 2005-07-26T11:00:00Z
modified: 2015-08-31T04:08:25Z
owned by: james
owned by: myers
mutable description: Tet-repressible production of CI(ts) to allow tuning of temperature readouts
discontinued: false
dominant: false
group u list: _38_
m user id: 0
owner id: 341
owning group id: 38
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Andrew Hessel
type: DnaRegion
role: SO:0000110
role: partType/Temporary
component: BBa_B0010
component: BBa_J06503
component: BBa_B0030
component: BBa_R0040
component: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0030
sequence annotation: BBa_J06503
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J11013_sequence/1

BBa_B0010
type: Component
persistent identity: component1666642
display id: component1666642
version: 1
top level: BBa_J11013
definition: BBa_B0010
access: public

BBa_J06503
type: Component
persistent identity: component1666637
display id: component1666637
version: 1
top level: BBa_J11013
definition: cI857
access: public

BBa_B0030
type: Component
persistent identity: component1666619
display id: component1666619
version: 1
top level: BBa_J11013
definition: BBa_B0030
access: public

BBa_R0040
type: Component
persistent identity: component1666608
display id: component1666608
version: 1
top level: BBa_J11013
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component1666652
display id: component1666652
version: 1
top level: BBa_J11013
definition: BBa_B0012
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1666608
display id: annotation1666608
version: 1
top level: BBa_J11013
location: https://synbiohub.org/public/igem/BBa_J11013/annotation1666608/range1666608/1
component: BBa_R0040

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type: Range
persistent identity: range1666608
display id: range1666608
version: 1
top level: BBa_J11013
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1666619
display id: annotation1666619
version: 1
top level: BBa_J11013
location: https://synbiohub.org/public/igem/BBa_J11013/annotation1666619/range1666619/1
component: BBa_B0030

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type: Range
persistent identity: range1666619
display id: range1666619
version: 1
top level: BBa_J11013
direction: direction/forward
start: 63
end: 77
orientation: inline

BBa_J06503
type: SequenceAnnotation
persistent identity: annotation1666637
display id: annotation1666637
version: 1
top level: BBa_J11013
location: https://synbiohub.org/public/igem/BBa_J11013/annotation1666637/range1666637/1
component: BBa_J06503

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type: Range
persistent identity: range1666637
display id: range1666637
version: 1
top level: BBa_J11013
direction: direction/forward
start: 84
end: 858
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1666642
display id: annotation1666642
version: 1
top level: BBa_J11013
location: https://synbiohub.org/public/igem/BBa_J11013/annotation1666642/range1666642/1
component: BBa_B0010

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type: Range
persistent identity: range1666642
display id: range1666642
version: 1
top level: BBa_J11013
direction: direction/forward
start: 867
end: 946
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1666652
display id: annotation1666652
version: 1
top level: BBa_J11013
location: https://synbiohub.org/public/igem/BBa_J11013/annotation1666652/range1666652/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J11013/annotation1666652/range1666652/1
type: Range
persistent identity: range1666652
display id: range1666652
version: 1
top level: BBa_J11013
direction: direction/forward
start: 955
end: 995
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

Prm +
type: ComponentDefinition
persistent identity: BBa I12007
display id: BBa_I12007
version: 1
was generated by: iGEM to SBOL conversion
description: Modified lambda Prm promoter (OR-3 obliterated)
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Shih and Gussin (1983)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI)
discontinued: false
dominant: true
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In wild type lambda phage, the OR3 site (-27 to -11) reads "tatcccttgcggtgata" on the sense strand of DNA. To prevent lambda repressor (cI) from binding to this site, the 4th through 10th nt of OR3 were replaced by the 7 nt between OR2 and OR1, "aaatagt" (-57 to -51), which in effect mutates 5 nt of the promoter. These nt were chosen to be about halfway between the -10 and -35 boxes of the promoter. Furthermore, since these nt already act as a spacer in wild-type phage, it is hoped they will not create undesired interactions.
star: true
creator: Hans
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: OR2
sequence annotation: -35
sequence annotation: mutate to obliterate OR3
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I12007_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation937701
display id: annotation937701
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1
type: Range
persistent identity: range937701
display id: range937701
version: 1
top level: Prm +
direction: direction/forward
start: 9
end: 25
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation937702
display id: annotation937702
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1
type: Range
persistent identity: range937702
display id: range937702
version: 1
top level: Prm +
direction: direction/forward
start: 33
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation937703
display id: annotation937703
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1
type: Range
persistent identity: range937703
display id: range937703
version: 1
top level: Prm +
direction: direction/forward
start: 48
end: 53
orientation: inline

mutate to obliterate OR3
type: SequenceAnnotation
persistent identity: annotation937704
display id: annotation937704
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1
type: Range
persistent identity: range937704
display id: range937704
version: 1
top level: Prm +
direction: direction/forward
start: 59
end: 65
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation937705
display id: annotation937705
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1
type: Range
persistent identity: range937705
display id: range937705
version: 1
top level: Prm +
direction: direction/forward
start: 71
end: 76
orientation: inline

BBa_B0031
type: ComponentDefinition
persistent identity: BBa B0031
display id: BBa_B0031
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.2 (weak) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0031_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23316
display id: annotation23316
version: 1
top level: BBa_B0031
location: https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
type: Range
persistent identity: range23316
display id: range23316
version: 1
top level: BBa_B0031
direction: direction/forward
start: 7
end: 10
orientation: inline

cI857
type: ComponentDefinition
persistent identity: BBa J06503
display id: BBa_J06503
version: 1
was generated by: iGEM to SBOL conversion
description: Lambda cI857 (Lambda II) (RBS- LVA+)
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:18Z
mutable provenance: Hendrix, Roger W. et al., ed. Lambda II. New York: Cold Spring Harbor Laboratory, 1983. ind- mutation on p. 626 (at bp 37589 of Lambda) 857 mutation on p. 628 (at bp 37742 of Lambda) Made by introducing two point mutations to Lambda cI (BBa_C0051): G(352)->A for ind- and G(199)->A for 857 temperature sensitivity.
owned by: james
owned by: myers
mutable description: Mutated from BBa_C0051 Repressor, Lambda cI (RBS- LVA+) to be temperature sensitive and UV insensitive.
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: First attempt to mutate failed. Suspect the problem is a low mutation rate due to results of positive control. May be a result of the mutagenesis kit being old. Second attempt to mutate succeeded. However, the sequencing had a lot of bad sequence at the beginings, so that the ind- mutation was not sequenced by either of the primers used. The ind- mutation was verified by the presence of a HindIII restrition site. This part was never completely sequenced. The cI857 QPI was made by Blue Heron, and this was part was abandoned.
star: false
creator: ytwang
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: cI857
sequence annotation: G->A (857)
sequence annotation: G->A (ind-)
sequence annotation: LVA
sequence annotation: barcode from LacI-LVA (BBa_C0012)
sequence: https://synbiohub.org/public/igem/BBa_J06503_sequence/1

cI857
type: SequenceAnnotation
persistent identity: annotation1549975
display id: annotation1549975
version: 1
top level: cI857
location: https://synbiohub.org/public/igem/BBa_J06503/annotation1549975/range1549975/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J06503/annotation1549975/range1549975/1
type: Range
persistent identity: range1549975
display id: range1549975
version: 1
top level: cI857
direction: direction/forward
start: 1
end: 711
orientation: inline

G->A (857)
type: SequenceAnnotation
persistent identity: annotation1549980
display id: annotation1549980
version: 1
top level: cI857
location: https://synbiohub.org/public/igem/BBa_J06503/annotation1549980/range1549980/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J06503/annotation1549980/range1549980/1
type: Range
persistent identity: range1549980
display id: range1549980
version: 1
top level: cI857
direction: direction/forward
start: 199
end: 199
orientation: inline

G->A (ind-)
type: SequenceAnnotation
persistent identity: annotation1549981
display id: annotation1549981
version: 1
top level: cI857
location: https://synbiohub.org/public/igem/BBa_J06503/annotation1549981/range1549981/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J06503/annotation1549981/range1549981/1
type: Range
persistent identity: range1549981
display id: range1549981
version: 1
top level: cI857
direction: direction/forward
start: 352
end: 352
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1549979
display id: annotation1549979
version: 1
top level: cI857
location: https://synbiohub.org/public/igem/BBa_J06503/annotation1549979/range1549979/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J06503/annotation1549979/range1549979/1
type: Range
persistent identity: range1549979
display id: range1549979
version: 1
top level: cI857
direction: direction/forward
start: 712
end: 744
orientation: inline

barcode from LacI-LVA (BBa_C0012)
type: SequenceAnnotation
persistent identity: annotation1552049
display id: annotation1552049
version: 1
top level: cI857
location: https://synbiohub.org/public/igem/BBa_J06503/annotation1552049/range1552049/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_J06503/annotation1552049/range1552049/1
type: Range
persistent identity: range1552049
display id: range1552049
version: 1
top level: cI857
direction: direction/forward
start: 751
end: 775
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

https://synbiohub.org/public/igem/BBa_J11014_sequence/1
type: Sequence
persistent identity: BBa J11014 sequence
display id: BBa_J11014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaacctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaagtactagatgtccagattagataaaag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J11008_sequence/1
type: Sequence
persistent identity: BBa J11008 sequence
display id: BBa_J11008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaacctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J11011_sequence/1
type: Sequence
persistent identity: BBa J11011 sequence
display id: BBa_J11011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J11012_sequence/1
type: Sequence
persistent identity: BBa J11012 sequence
display id: BBa_J11012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaagtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J11013_sequence/1
type: Sequence
persistent identity: BBa J11013 sequence
display id: BBa_J11013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttacaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctaagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06503_sequence/1
type: Sequence
persistent identity: BBa J06503 sequence
display id: BBa_J06503_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttacaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctaagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12007_sequence/1
type: Sequence
persistent identity: BBa I12007 sequence
display id: BBa_I12007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0031_sequence/1
type: Sequence
persistent identity: BBa B0031 sequence
display id: BBa_B0031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z