##gff-version 3
##sequence-region BBa_J11018 1 1896
BBa_J11018	.	sequence_feature	1	921	.	+	0	ID=BBa_J11016;Name=BBa_J11016
BBa_J11018	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_J11016
BBa_J11018	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J11018	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J11018	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J11018	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J11018	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_J11018	.	CDS	79	759	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J11016
BBa_J11018	.	engineered_tag	760	784	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J11018	.	CDS	79	753	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J11018	.	ribosome_entry_site	58	72	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J11016
BBa_J11018	.	ribosome_entry_site	65	69	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J11018	.	engineered_region	58	72	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J11018	.	ribosome_entry_site	58	72	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J11018	.	terminator	793	872	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J11016
BBa_J11018	.	engineered_region	793	872	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J11018	.	stem_loop	804	847	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J11018	.	terminator	881	921	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J11016
BBa_J11018	.	stop_codon	914	914	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J11018	.	polyA_site	908	921	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J11018	.	stem_loop	888	907	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J11018	.	engineered_region	881	921	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J11018	.	sequence_feature	930	1896	.	+	0	ID=BBa_J11017;Name=BBa_J11017
BBa_J11018	.	terminator	1856	1896	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J11017
BBa_J11018	.	stop_codon	1889	1889	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J11018	.	polyA_site	1883	1896	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J11018	.	stem_loop	1863	1882	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J11018	.	engineered_region	1856	1896	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J11018	.	ribosome_entry_site	1020	1033	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J11017
BBa_J11018	.	conserved_region	1026	1029	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J11018	.	promoter	930	1011	.	+	0	ID=BBa_I12007;Name=BBa_I12007;Parent=BBa_J11017
BBa_J11018	.	promoter	1000	1005	.	+	0	ID=annotation937705;Name=-10;Parent=BBa_I12007
BBa_J11018	.	non_covalent_binding_site	962	978	.	+	0	ID=annotation937702;Name=OR2;Parent=BBa_I12007
BBa_J11018	.	non_covalent_binding_site	938	954	.	+	0	ID=annotation937701;Name=OR1;Parent=BBa_I12007
BBa_J11018	.	sequence_alteration	988	994	.	+	0	ID=annotation937704;Name=mutate to obliterate OR3;Parent=BBa_I12007
BBa_J11018	.	promoter	977	982	.	+	0	ID=annotation937703;Name=-35;Parent=BBa_I12007
BBa_J11018	.	CDS	1040	1759	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_J11017
BBa_J11018	.	CDS	1040	1759	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J11018	.	terminator	1768	1847	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J11017
BBa_J11018	.	engineered_region	1768	1847	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J11018	.	stem_loop	1779	1822	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_J11018
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagattaaagaggagaaatactaga
tggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagtt
cgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccg
ctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcc
cggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttac
cgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgac
ggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaag
gtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaa
accggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgtt
gaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaaccattatcaccgcca
gaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagagtcacacag
gaaacctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgtta
atgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcac
tactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagataccca
gatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaag
atgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtat
tgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggca
gacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagacc
attatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccct
ttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatg
gatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata