LOCUS BBa_J11020 3679 bp DNA linear 21-Mar-2025 DEFINITION Tunable Temperature Sensor ACCESSION BBa_J11020 VERSION BBa_J11020.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 misc_feature 64..67 /label=conserved RBS 58..71 /label=BBa_B0031 CDS 78..797 /label=GFP protein CDS 78..797 /label=BBa_E0040 stem_loop 817..860 /label=stem_loop misc_feature 806..885 /label=BBa_B0010 terminator 806..885 /label=BBa_B0010 stem_loop 901..920 /label=T7 TE misc_feature 927..927 /label=stop polyA_site 921..934 /label=polya misc_feature 894..934 /label=BBa_B0012 terminator 894..934 /label=BBa_B0012 misc_feature 951..967 /label=OR1 misc_feature 975..991 /label=OR2 promoter 990..995 /label=-35 misc_feature 1001..1007 /label=mutate to obliterate OR3 promoter 1013..1018 /label=-10 promoter 943..1024 /label=BBa_I12007 RBS 1033..1047 /label=RBS-1\Strong RBS 1040..1044 /label=RBS misc_feature 1033..1047 /label=BBa_B0030 RBS 1033..1047 /label=BBa_B0030 CDS 1054..1764 /label=cI 857 misc_feature 1765..1797 /label=LVA CDS 1054..1803 /label=BBa_C0081 CDS 1810..2484 /label=mrfp1 misc_feature 2491..2515 /label=Help:Barcodes CDS 1810..2490 /label=BBa_E1010 stem_loop 2535..2578 /label=stem_loop misc_feature 2524..2603 /label=BBa_B0010 terminator 2524..2603 /label=BBa_B0010 stem_loop 2619..2638 /label=T7 TE misc_feature 2645..2645 /label=stop polyA_site 2639..2652 /label=polya misc_feature 2612..2652 /label=BBa_B0012 terminator 2612..2652 /label=BBa_B0012 misc_feature 2661..2679 /label=TetR 1 misc_feature 2661..2714 /label=BBa_R0040 promoter 2680..2685 /label=-35 misc_feature 2686..2704 /label=TetR 2 promoter 2703..2708 /label=-10 promoter 2661..2714 /label=BBa_R0040 RBS 2723..2737 /label=RBS-1\Strong RBS 2730..2734 /label=RBS misc_feature 2723..2737 /label=BBa_B0030 RBS 2723..2737 /label=BBa_B0030 CDS 2744..3454 /label=cI 857 misc_feature 3455..3487 /label=LVA CDS 2744..3493 /label=BBa_C0081 stem_loop 3513..3556 /label=stem_loop misc_feature 3502..3581 /label=BBa_B0010 terminator 3502..3581 /label=BBa_B0010 stem_loop 3597..3616 /label=T7 TE misc_feature 3623..3623 /label=stop polyA_site 3617..3630 /label=polya misc_feature 3590..3630 /label=BBa_B0012 terminator 3590..3630 /label=BBa_B0012 stem_loop 3646..3665 /label=T7 TE misc_feature 3672..3672 /label=stop polyA_site 3666..3679 /label=polya misc_feature 3639..3679 /label=BBa_B0012 terminator 3639..3679 /label=BBa_B0012 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca 61 cacaggaaac ctactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc 121 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag 181 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg 241 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc 301 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg 361 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg 421 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa 481 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag 541 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa 601 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt 661 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga 721 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg 781 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 841 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 901 ggctcacctt cgggtgggcc tttctgcgtt tatatactag aggcaaccat tatcaccgcc 961 agaggtaaaa tagtcaacac gcacggtgtt agatatttat aaatagtggt gatagattta 1021 acgttactag agattaaaga ggagaaatac tagatgagca caaaaaagaa accattaaca 1081 caagagcagc ttgaggacgc acgtcgcctt aaagcaattt atgaaaaaaa gaaaaatgaa 1141 cttggcttat cccaggaatc tgtcgcagac aagatgggga tggggcagtc aggcgttggt 1201 gctttattta atggcatcaa tgcattaaat gcttataacg ccgcattgct tgcaaaaatt 1261 ctcaaagtta gcgttgaaga atttagccct tcaatcgcca cagaaatcta cgagatgtat 1321 gaagcggtta gtatgcagcc gtcacttaga agtgagtatg agtaccctgt tttttctcat 1381 gttcaggcag ggatgttctc acctaagctt agaaccttta ccaaaggtga tgcggagaga 1441 tgggtaagca caaccaaaaa agccagtgat tctgcattct ggcttgaggt tgaaggtaat 1501 tccatgaccg caccaacagg ctccaagcca agctttcctg acggaatgtt aattctcgtt 1561 gaccctgagc aggctgttga gccaggtgat ttctgcatag ccagacttgg gggtgatgag 1621 tttaccttca agaaactgat cagggatagc ggtcaggtgt ttttacaacc actaaaccca 1681 cagtacccaa tgatcccatg caatgagagt tgttccgttg tggggaaagt tatggctagt 1741 cagtggcctg aagagacgtt tggcgctgca aacgacgaaa actacgcttt agtagcttaa 1801 taatactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt 1861 atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg 1921 tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct 1981 tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct 2041 gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg 2101 aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag 2161 ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag 2221 aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg 2281 aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt 2341 aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc 2401 aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct 2461 gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag atcgctacta 2521 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 2581 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 2641 tctgcgttta tatactagag tccctatcag tgatagagat tgacatccct atcagtgata 2701 gagatactga gcactactag agattaaaga ggagaaatac tagatgagca caaaaaagaa 2761 accattaaca caagagcagc ttgaggacgc acgtcgcctt aaagcaattt atgaaaaaaa 2821 gaaaaatgaa cttggcttat cccaggaatc tgtcgcagac aagatgggga tggggcagtc 2881 aggcgttggt gctttattta atggcatcaa tgcattaaat gcttataacg ccgcattgct 2941 tgcaaaaatt ctcaaagtta gcgttgaaga atttagccct tcaatcgcca cagaaatcta 3001 cgagatgtat gaagcggtta gtatgcagcc gtcacttaga agtgagtatg agtaccctgt 3061 tttttctcat gttcaggcag ggatgttctc acctaagctt agaaccttta ccaaaggtga 3121 tgcggagaga tgggtaagca caaccaaaaa agccagtgat tctgcattct ggcttgaggt 3181 tgaaggtaat tccatgaccg caccaacagg ctccaagcca agctttcctg acggaatgtt 3241 aattctcgtt gaccctgagc aggctgttga gccaggtgat ttctgcatag ccagacttgg 3301 gggtgatgag tttaccttca agaaactgat cagggatagc ggtcaggtgt ttttacaacc 3361 actaaaccca cagtacccaa tgatcccatg caatgagagt tgttccgttg tggggaaagt 3421 tatggctagt cagtggcctg aagagacgtt tggcgctgca aacgacgaaa actacgcttt 3481 agtagcttaa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 3541 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 3601 caccttcggg tgggcctttc tgcgtttata tactagagtc acactggctc accttcgggt 3661 gggcctttct gcgtttata //