LOCUS BBa_J11022 5441 bp DNA linear 11-May-2024 DEFINITION Tunable Temperature Sensor v3 (Registry 7.05 DNA-1 + Harvard Parts) ACCESSION BBa_J11022 VERSION BBa_J11022.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..2364 /label=LacI(ts)-LVA misc_feature 2039..2039 /label=TS mutation (G->A) misc_feature 2326..2364 /label=LVA misc_feature 2365..2389 /label=barcode from LacI (BBa_C0012) CDS 1237..2389 /label=BBa_J06501 stem_loop 2409..2452 /label=stem_loop misc_feature 2398..2477 /label=BBa_B0010 terminator 2398..2477 /label=BBa_B0010 stem_loop 2493..2512 /label=T7 TE misc_feature 2519..2519 /label=stop polyA_site 2513..2526 /label=polya misc_feature 2486..2526 /label=BBa_B0012 terminator 2486..2526 /label=BBa_B0012 misc_feature 2537..2553 /label=lac O1 promoter 2554..2559 /label=-35 misc_feature 2560..2576 /label=lac O1 promoter 2577..2582 /label=-10 misc_feature 2535..2588 /label=BBa_R0011 promoter 2535..2588 /label=BBa_R0011 misc_feature 2604..2607 /label=conserved RBS 2598..2611 /label=BBa_B0031 misc_feature 3248..3253 /label=2 CDS 2618..3253 /label=cI 434 CDS 2618..3253 /label=BBa_C0056 stem_loop 3273..3316 /label=stem_loop misc_feature 3262..3341 /label=BBa_B0010 terminator 3262..3341 /label=BBa_B0010 stem_loop 3357..3376 /label=T7 TE misc_feature 3383..3383 /label=stop polyA_site 3377..3390 /label=polya misc_feature 3350..3390 /label=BBa_B0012 terminator 3350..3390 /label=BBa_B0012 CDS 3399..3486 /label=end of LacI coding region (inactive) misc_feature 3399..3598 /label=BBa_R0010 misc_feature 3487..3524 /label=CAP binding site promoter 3535..3540 /label=-35 promoter 3559..3564 /label=-10 misc_feature 3564..3598 /label=LacI binding site misc_feature 3571..3571 /label=start promoter 3399..3598 /label=BBa_R0010 misc_feature 3611..3614 /label=conserved RBS 3607..3618 /label=BBa_B0034 CDS 3625..4299 /label=mrfp1 misc_feature 4306..4330 /label=Help:Barcodes CDS 3625..4305 /label=BBa_E1010 stem_loop 4350..4393 /label=stem_loop misc_feature 4339..4418 /label=BBa_B0010 terminator 4339..4418 /label=BBa_B0010 stem_loop 4434..4453 /label=T7 TE misc_feature 4460..4460 /label=stop polyA_site 4454..4467 /label=polya misc_feature 4427..4467 /label=BBa_B0012 terminator 4427..4467 /label=BBa_B0012 misc_feature 4531..4544 /label=OR1 434 misc_feature 4484..4500 /label=OR1 lambda misc_feature 4508..4524 /label=OR2 lambda promoter 4546..4551 /label=-10 promoter 4523..4528 /label=-35 promoter 4476..4557 /label=BBa_I12006 RBS 4566..4578 /label=RBS-3\Weak RBS 4572..4575 /label=RBS misc_feature 4566..4578 /label=BBa_B0032 RBS 4566..4578 /label=BBa_B0032 CDS 4585..5304 /label=GFP protein CDS 4585..5304 /label=BBa_E0040 stem_loop 5324..5367 /label=stem_loop misc_feature 5313..5392 /label=BBa_B0010 terminator 5313..5392 /label=BBa_B0010 stem_loop 5408..5427 /label=T7 TE misc_feature 5434..5434 /label=stop polyA_site 5428..5441 /label=polya misc_feature 5401..5441 /label=BBa_B0012 terminator 5401..5441 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg 1261 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 1321 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 1381 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc 1441 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 1501 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 1561 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 1621 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 1681 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 1741 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 1801 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 1861 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 1921 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 1981 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttgat 2041 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 2101 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 2161 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 2221 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 2281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa 2341 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagcca 2401 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2461 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2521 tttatatact agagaattgt gagcggataa caattgacat tgtgagcgga taacaagata 2581 ctgagcacat actagagtca cacaggaaac ctactagatg agtatttctt ccagggtaaa 2641 aagcaaaaga atccagcttg gacttaacca ggctgaactt gctcaaaagg tggggactac 2701 ccagcagtct atagagcagc tcgaaaacgg taaaactaag cgaccacgct ttttaccaga 2761 acttgcgtca gctcttggcg taagtgttga ctggctgctc aatggcacct ctgattcgaa 2821 tgttagattt gttgggcacg ttgagcccaa agggaaatat ccattgatta gcatggttag 2881 agctggttcg tggtgtgaag cttgtgaacc ctacgatatc aaggacattg atgaatggta 2941 tgacagtgac gttaacttat taggcaatgg attctggctg aaggttgaag gtgattccat 3001 gacctcacct gtaggtcaaa gcatccctga aggtcatatg gtgttagtag atactggacg 3061 ggagccagtg aatggaagcc ttgttgtagc caaactgact gacgcgaacg aagcaacatt 3121 caagaaactg gtcatagatg gcggtcagaa gtacctgaaa ggcctgaatc cttcatggcc 3181 tatgactcct atcaacggaa actgcaagat tatcggtgtt gtcgtggaag cgagggtaaa 3241 attcgtataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 3301 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 3361 caccttcggg tgggcctttc tgcgtttata tactagagca atacgcaaac cgcctctccc 3421 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 3481 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 3541 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacata 3601 ctagagaaag aggagaaata ctagatggct tcctccgaag acgttatcaa agagttcatg 3661 cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa 3721 ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt 3781 ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac 3841 gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa 3901 tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc 3961 ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac 4021 ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg 4081 gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac 4141 tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct 4201 tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa 4261 cagtacgaac gtgctgaagg tcgtcactcc accggtgctt aataacgctg atagtgctag 4321 tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 4381 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 4441 cttcgggtgg gcctttctgc gtttatatac tagaggcaac cattatcacc gccagaggta 4501 aaatagtcaa cacgcacggt gttagatatt acaaactttc ttgtatagat ttaacgttac 4561 tagagtcaca caggaaagta ctagatgcgt aaaggagaag aacttttcac tggagttgtc 4621 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag 4681 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa 4741 ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg 4801 agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat 4861 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc 4921 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa 4981 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc 5041 atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa 5101 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct 5161 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac 5221 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc 5281 atggatgaac tatacaaata ataatactag agccaggcat caaataaaac gaaaggctca 5341 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 5401 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //