LOCUS       BBa_J11030              1431 bp    DNA     linear       09-May-2024
DEFINITION  Bacterial Whiteboard by Hannah
ACCESSION   BBa_J11030
VERSION     BBa_J11030.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..428
                     /label=lacZ alpha
     misc_feature    161..161
                     /label=T
     misc_feature    167..167
                     /label=A
     misc_feature    188..188
                     /label=C
     misc_feature    158..158
                     /label=C
     misc_feature    191..191
                     /label=G
     promoter        106..115
                     /label=T3 promoter
     promoter        253..271
                     /label=T7 promoter
     misc_feature    279..428
                     /label=lacZ gene fragment
     misc_feature    81..95
                     /label=lacZ gene fragment
     misc_feature    81..433
                     /label=BBa_E0033
     stem_loop       453..496
                     /label=stem_loop
     misc_feature    442..521
                     /label=BBa_B0010
     terminator      442..521
                     /label=BBa_B0010
     stem_loop       537..556
                     /label=T7 TE
     misc_feature    563..563
                     /label=stop
     polyA_site      557..570
                     /label=polya
     misc_feature    530..570
                     /label=BBa_B0012
     terminator      530..570
                     /label=BBa_B0012
     terminator      442..570
                     /label=BBa_B0015
     misc_feature    63..570
                     /label=BBa_I2012
     misc_feature    581..597
                     /label=lac O1
     promoter        598..603
                     /label=-35
     misc_feature    604..620
                     /label=lac O1
     promoter        621..626
                     /label=-10
     misc_feature    579..632
                     /label=BBa_R0011
     promoter        579..632
                     /label=BBa_R0011
     RBS             642..656
                     /label=RBS-1\Strong
     RBS             649..653
                     /label=RBS
     misc_feature    642..656
                     /label=BBa_B0030
     RBS             642..656
                     /label=BBa_B0030
     CDS             663..1382
                     /label=GFP protein
     CDS             663..1382
                     /label=BBa_E0040
     stem_loop       1398..1417
                     /label=T7 TE
     misc_feature    1424..1424
                     /label=stop
     polyA_site      1418..1431
                     /label=polya
     misc_feature    1391..1431
                     /label=BBa_B0012
     terminator      1391..1431
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaccatga ttacgccaag cgcgcaatta accctcacta
      121 aagggaacaa aagctggagc tccaccgcgg tggcggcagc actagagcta gtggatcccc
      181 cgggctgtag aaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg
      241 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
      301 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      361 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      421 gcctgaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      481 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      541 caccttcggg tgggcctttc tgcgtttata tactagagaa ttgtgagcgg ataacaattg
      601 acattgtgag cggataacaa gatactgagc acatactaga gattaaagag gagaaatact
      661 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      721 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      781 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      841 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
      901 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
      961 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
     1021 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
     1081 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
     1141 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
     1201 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     1261 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
     1321 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
     1381 aatactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//